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We narrowed to 346 results for: pET3

Showing: 261 - 280 of 346 results

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pETMutSdelta768

Plasmid

#13041

Depositor

Peggy Hsieh

Article

Biswas et al J Mol Biol. 2001 Jan 26. 305(4):805-16.

Insert

MutS

Use

Tags

Expression

Bacterial

Mutation

deleted C-terminal aa residues 769 - 811

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
GSPD1_R252L

Plasmid

#224153

Purpose

IPTG-inducible expression of C. elegans protein GSPD-1(R252L) with a His tag

Depositor

Katherine Walstrom

Article

Loges et al MicroPubl Biol. 2021 Sep 10;2021:10.1791

Insert

GSPD-1(R252L)

Use

Tags

LeuGlu-6xHis

Expression

Bacterial

Mutation

R252L

Promoter

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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GSPD1_AJ

Plasmid

#224152

Purpose

IPTG-inducible expression of C. elegans protein GSPD-1 with a His tag

Depositor

Katherine Walstrom

Article

Loges et al MicroPubl Biol. 2021 Sep 10;2021:10.1791

Insert

GSPD-1 (B0035.5 Nematode)

Use

Tags

LeuGlu-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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GSPD1_D60N

Plasmid

#224154

Purpose

IPTG-inducible expression of C. elegans protein GSPD-1(D60N) with a His tag

Depositor

Katherine Walstrom

Article

Loges et al MicroPubl Biol. 2021 Sep 10;2021:10.1791

Insert

GSPD-1(D60N)

Use

Tags

LeuGlu-6xHis

Expression

Bacterial

Mutation

D60N

Promoter

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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POC1468

Plasmid

#213756

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Non-switchable methylase M2.BsaI

Use

Tags

His6-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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His6TEV-UbG76C

Plasmid

#75299

Purpose

Bacteria expression plasmid for Ubiquitin G76C with removable His6 tag by TEV protease

Depositor

Cynthia Wolberger

Article

Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

Insert

human Ubiquitin mutant G76 to C

Use

Tags

His

Expression

Bacterial

Mutation

changed Glycine 76 to Cystine

Promoter

T7

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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H2BK120C

Plasmid

#75332

Purpose

Bacteria expression of H2BK120C

Depositor

Cynthia Wolberger

Article

Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

Insert

H2BK120C (h2bc11.S Frog)

Use

Tags

none

Expression

Bacterial

Mutation

truncated; K120C

Promoter

T7

Available since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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His-PaIct

Plasmid

#111293

Purpose

bacterial expression

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

succinyl-CoA:itaconate CoA transferase

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET-3a-His-MBP-TEV-VC_Cas1_WT

Plasmid

#120364

Purpose

Expresses wildtype V-C Cas1

Depositor

Jennifer Doudna

Article

Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

Insert

VC_Cas1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET-3a-His-MBP-TEV-VD_Cas1_WT

Plasmid

#120365

Purpose

Expresses wildtype V-D Cas1

Depositor

Jennifer Doudna

Article

Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

Insert

VD_Cas1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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UbG75C

Plasmid

#37194

Depositor

Cynthia Wolberger

Article

Wiener et al Nature. 2012 Feb 22;483(7391):618-22. do

Insert

Ubiquitin G75C mutant (RBX1 Human)

Use

Tags

No tag

Expression

Bacterial

Mutation

Glycine 75 changed to Cystine

Promoter

T7

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only
hP14H8y

Plasmid

#84368

Purpose

antigen for human PRDM14 purification ( aa 1-243)

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

PRDM14 (PRDM14 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

contains PRDM14 AA1-243

Promoter

T7

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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His8-TEV-PseC

Plasmid

#89724

Purpose

Expresses PseC from C. jejuni with an N-terminal octaHis tag and TEV cleavage site

Depositor

Barbara Imperiali

Article

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

PseC (pseC Campylobacter jejuni)

Use

Tags

His8 and TEV

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only
POC1486

Plasmid

#213811

Purpose

BpiI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BpiI-associated non-switchable methylase M2.HpyAII

Use

Tags

His6-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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POC1464

Plasmid

#213736

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Non-switchable methylase M2.Eco31I

Use

Tags

His6-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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POC1474

Plasmid

#213762

Purpose

BpiI-associated switch methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BpiI-associated switch methylase M2.NmeMC58II

Use

Tags

His6-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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POC1488

Plasmid

#213813

Purpose

BpiI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BpiI-associated non-switchable methylase M1.MboII

Use

Tags

His6-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1484

Plasmid

#213810

Purpose

BpiI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BpiI-associated non-switchable methylase M1.HpyAII

Use

Tags

His6-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1466

Plasmid

#213754

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Truncated non-switchable methylase M2.Eco31I

Use

Tags

His6-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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POC1472

Plasmid

#213760

Purpose

BsaI-associated switch methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BsaI-associated switch methylase M.Osp807II

Use

Tags

His6-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge