We narrowed to 1,451 results for: transfection

1. pNHT7::RPL15::sGluc

   Plasmid

   #186769

   Purpose

   An mRNA production vector encoding planarian codon-optimized gaussia luciferase (sGluc) with RPL15 5' and 3' UTRs.

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   Gaussia luciferase
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CMV-pDisplay-Halo-T2A-EGFP

   Plasmid

   #223498

   Purpose

   Co-expression of HaloTags to outer surface in mammalian cells coupled with a EGFP transfection marker

   Depositor

   James Frank

   Insert

   HaloTag and EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. hSyn-pDisplay-SNAP-T2A-EGFP

   Plasmid

   #223503

   Purpose

   Co-expression of SNAP-tags to outer surface in mammalian cells coupled with an EGFP transfection marker

   Depositor

   James Frank

   Insert

   SNAP-tag and EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTM-RBDv2

   Plasmid

   #162785

   Purpose

   SARS-CoV-2 S protein receptor binding domain (RBD) expression in mammalian cells

   Depositor

   Ivan Vorobiev

   Article

   Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi

   Insert

   SARS-CoV-2 S protein receptor binding domain (RBD) (S Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2))
   

   Use

   Tags

   10xHis, c-myc, and hTPA leader

   Expression

   Mammalian

   Mutation

   Promoter

   CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

   Available since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti_P2A_Blast

   Plasmid

   #211365

   Purpose

   Lentiviral expression vector for an insert of interest linked to a P2A-T2A blasticidin sequence. Produces virus very efficiently. Can also be used for regular transfections

   Depositor

   Jacques Neefjes

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDmelOR

   Plasmid

   #126476

   Purpose

   Vector for the functional expression of insect ORs in mammalian cells, with a human codon-optimized version of D.melanogaster Orco (hOrco) with an N-terminal myc tag and a β-globin/IgG chimeric intron

   Depositor

   Bill Hansson

   Article

   Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

   Insert

   DmelOrco (Orco Fly)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   codon optimization for H. sapiens; includes a rec…

   Promoter

   CMV

   Available since

   June 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(-)-mRho.V5.mER.hOr47a

   Plasmid

   #126472

   Purpose

   Human codon-optimized version of D. melanogaster Or47a (hOr47a) with N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved intracellular trafficking in mammalian cells

   Depositor

   Bill Hansson

   Article

   Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

   Insert

   Or47a (Or47a Fly)
   

   Use

   Tags

   H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…

   Expression

   Mammalian

   Mutation

   codon optimization for H. sapiens

   Promoter

   CMV

   Available since

   June 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAID1.2-EF1a-NmClover3-hsLem2

   Plasmid

   #179524

   Purpose

   Express OsTIR1 and mClover3-mAID-hsLem2 in mammalian cells under the control of EF1a promoter

   Depositor

   Yasushi Hiraoka

   Article

   Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

   Insert

   LEMD2 (LEMD2 Human)
   

   Use

   Tags

   mClover3-mAID

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. hSyn-pDisplay-SNAP[C145A]-T2A-EGFP

   Plasmid

   #223504

   Purpose

   Co-expression of dead-mutant SNAP-tags to outer surface in mammalian cells coupled with an EGFP transfection marker

   Depositor

   James Frank

   Insert

   dead-mutant SNAP-tag and EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SNAP-tag C145A

   Promoter

   hSyn

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pT2iD6Hoxa13bER4(#686)

    Plasmid

    #184101

    Purpose

    cyclofen-inducible fish Hoxa13b activation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Insert

    ZfHoxa13b-ERT2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    sCMV+SP6

    Available since

    July 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pT2iD6Hoxa13aER4(#685)

    Plasmid

    #184100

    Purpose

    cyclofen-inducible fish Hoxa13a activation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Insert

    ZfHoxa13a-ERT2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    sCMV+SP6

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDmelOR-mRho.V5.mER.hOr56a

    Plasmid

    #126479

    Purpose

    Human codon-optimized D. melanogaster Orco (hOrco) and Or56a (hOr56a). Or56a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells

    Depositor

    Bill Hansson

    Article

    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

    Inserts

    Or56a (Or56a Fly)
    Orco (Orco Fly)
    

    Use

    Tags

    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…

    Expression

    Mammalian

    Mutation

    codon optimization for H. sapiens and codon optim…

    Promoter

    CMV

    Available since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTM-RBDv2

    Plasmid

    #162785

    Purpose

    SARS-CoV-2 S protein receptor binding domain (RBD) expression in mammalian cells

    Depositor

    Ivan Vorobiev

    Article

    Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi

    Insert

    SARS-CoV-2 S protein receptor binding domain (RBD) (S Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2))
    

    Use

    Tags

    10xHis, c-myc, and hTPA leader

    Expression

    Mammalian

    Mutation

    Promoter

    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

    Available since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPGK-empty

    Plasmid

    #35094

    Depositor

    David Segal

    Article

    Bhakta et al Methods Mol Biol. 2010;649:3-30.

    Insert

    deletion of zinc fingers and nuclease
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    use as a transfection balance for pPGK-ZFN plasmi…

    Promoter

    PGK

    Available since

    May 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-E-Flag

    Plasmid

    #101858

    Purpose

    Evi-1 flag tagged for transfection into mammalian cells

    Depositor

    Archibald Perkins

    Insert

    Evi-1 (Mecom Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pT2iC6Dronpa2nlsER4(#636)

    Plasmid

    #184067

    Purpose

    cyclofen-inducible Dronpa2 nuclear relocation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Insert

    Dronpa2-2nls-ERT2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    sCMV+SP6

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pT2iC6FAST1nls2ER4(#638)

    Plasmid

    #184068

    Purpose

    cyclofen-inducible YFAST nuclear relocation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Insert

    YFAST-2nls-ERT2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    sCMV+SP6

    Available since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Clim Act MCH (CAM)

    Plasmid

    #104443

    Purpose

    Expresses mCherry under endogenous actin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.

    Depositor

    Elena Casacuberta, Inaki Ruiz-Trillo

    Insert

    mCherry
    

    Use

    Expression in corallochytrium (single-celled euka…

    Tags

    Expression

    Mutation

    Promoter

    actin

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Clim EF1 MCH (CEM)

    Plasmid

    #104444

    Purpose

    Expresses mCherry under elongation factor 1 promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.

    Depositor

    Elena Casacuberta, Inaki Ruiz-Trillo

    Insert

    mCherry
    

    Use

    Expression in corallochytrium (single-celled euka…

    Tags

    Expression

    Mutation

    Promoter

    EF1

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Clim Tub MCH (CTM)

    Plasmid

    #104445

    Purpose

    Expresses mCherry under tubulin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.

    Depositor

    Elena Casacuberta, Inaki Ruiz-Trillo

    Insert

    mCherry
    

    Use

    Expression in corallochytrium (single-celled euka…

    Tags

    Expression

    Mutation

    Promoter

    tubulin promoter

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge