We narrowed to 986 results for: transfection

1. SEC13 in pRK5

   Plasmid

   #154215

   Purpose

   Untagged SEC13 cDNA expression vector for transfections

   Depositor

   David Sabatini

   Article

   Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

   Insert

   Sec13
   

   Tags

   NONE

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pARv-RFP

   Plasmid

   #60021

   Purpose

   pARv-RFP is a fluorescent reporter plasmid, that allows fast and easy evaluation of ZFN, TALEN or CRISPR/Cas9 activity in the transfected cells.

   Depositor

   Radislav Sedláček

   Article

   Kasparek et al FEBS Lett. 2014 Sep 18. pii: S0014-5793(

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Nov. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. sgRNA AAVS1

   Plasmid

   #128119

   Purpose

   Co-expresses sgRNA AAVS1, SpyCas9 scaffold (F+E) and GFP (transfection marker)

   Depositor

   Dominik Niopek

   Article

   Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

   Insert

   sgRNA AAVS1 + GFP
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   RSV/U6

   Available Since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. sgRNA EMX1

   Plasmid

   #128121

   Purpose

   Co-expresses sgRNA EMX1, SpyCas9 scaffold (F+E) and GFP (transfection marker)

   Depositor

   Dominik Niopek

   Article

   Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

   Insert

   sgRNA EMX1 + GFP
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   RSV/U6

   Available Since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. TVMV Protease-mCherry

   Plasmid

   #243794

   Purpose

   Encodes for mammalian expression of TVMV protease input. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.

   Depositor

   Cole DeForest

   Article

   Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

   Insert

   TVMV protease
   

   Expression

   Mammalian

   Available Since

   Oct. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBOB-HA-Gnaq

   Plasmid

   #236750

   Purpose

   Express HA N-terminal tagged mouse Gq transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

   Depositor

   Zhao Zhang

   Article

   Jiang et al J Clin Invest. 2024 Aug 13;134(19):e1821

   Insert

   Gnaq (Gnaq Mouse)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Available Since

   May 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV-TO-g0 (FLP-IN)

   Plasmid

   #236120

   Purpose

   Plasmid encoding g0 guide RNA under control of CMV promoter with two TetR binding sites, used to equalize plasmid masses for transfection

   Depositor

   Xiaojing Gao

   Article

   Wolfsberg et al bioRxiv 2025.02.24.639918

   Insert

   g0
   

   Expression

   Mammalian

   Promoter

   CMV promoter with two TetR binding sites

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCBE-dGFP

   Plasmid

   #226861

   Purpose

   Constitutively expresses mCherry as a transfection marker. Expresses non-fluorescent EGFP, deactivated by Y93H mutation, that can be reactivated by active cytosine base editing

   Depositor

   Alexis Komor

   Article

   Osgood et al Methods in Enzymology, February 5, 2025

   Insert

   EGFP
   

   Expression

   Mammalian

   Mutation

   Y93H

   Promoter

   CMV

   Available Since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. Tol2-GgVCL/WT

   Plasmid

   #162787

   Purpose

   CONTROL_transfection of mammalian cells with WT Vcl under a β-actin promoter, with IRES-GFP allowing identification of transfected cells

   Depositor

   Laura Lafon-Hughes

   Article

   et al PeerJ

   Insert

   Gallus gallus vinculin (VCL Chicken)
   

   Tags

   IRES-GFP

   Expression

   Bacterial and Mammalian

   Mutation

   WT

   Promoter

   beta actin

   Available Since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. sgRNA HBB

    Plasmid

    #128120

    Purpose

    Co-expresses sgRNA HBB, SpyCas9 scaffold (F+E) and GFP (transfection marker)

    Depositor

    Dominik Niopek

    Article

    Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

    Insert

    sgRNA HBB + GFP
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    RSV/U6

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. sgRNA RUNX

    Plasmid

    #128122

    Purpose

    Co-expresses sgRNA RUNX, SpyCas9 scaffold (F+E) and GFP (transfection marker)

    Depositor

    Dominik Niopek

    Article

    Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

    Insert

    sgRNA RUNX + GFP
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    RSV/U6

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. sgRNA HEK

    Plasmid

    #128123

    Purpose

    Co-expresses sgRNA HEK, SpyCas9 scaffold (F+E) and GFP (transfection marker)

    Depositor

    Dominik Niopek

    Article

    Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

    Insert

    sgRNA HEK + GFP
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    RSV/U6

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Thy1.1-muSOX D219A

    Plasmid

    #131704

    Purpose

    encodes Thy1.1 followed by a P2A site and muSOX D219A catalytic mutant for enriching pure populations of transfected cells

    Depositor

    Britt Glaunsinger

    Article

    Gilbertson et al Elife. 2018 Oct 3;7. pii: 37663. doi: 10

    Insert

    Thy1.1-muSOX
    

    Expression

    Mammalian

    Mutation

    Aspartic acid 219 mutated to an alanine

    Promoter

    CMV

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBMN-dGBP1-TagBFP

    Plasmid

    #80087

    Purpose

    MMLV retroviral vector for expression of dGBP1-TagBFP with t-hcRed as an infection/transfection marker

    Depositor

    Connie Cepko

    Article

    Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

    Insert

    dGBP1-TagBFP
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Feb. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAS_4xMma PylT_GFP 150TAG 6xHis

    Plasmid

    #140015

    Purpose

    Plasmid with 4xMmaPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    sfGFP
    

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    150TAG

    Promoter

    EF1

    Available Since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. c-Fos

    Plasmid

    #187905

    Purpose

    Expresses c-Fos in transfected cells

    Depositor

    Jennifer Mitchell

    Article

    Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

    Insert

    cFos (FOS Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. TEV-HRV-3C Proteases-mCherry

    Plasmid

    #243795

    Purpose

    Encodes for mammalian expression of TEV and HRV-3C protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.

    Depositor

    Cole DeForest

    Article

    Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

    Insert

    TEV and HRV-3C proteases
    

    Expression

    Mammalian

    Available Since

    Oct. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. c-Jun

    Plasmid

    #187902

    Purpose

    Expresses c-Jun in transfected cells

    Depositor

    Jennifer Mitchell

    Article

    Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

    Insert

    c-Jun (JUN Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT7-TaGu-WT-3xF-ORF

    Plasmid

    #238487

    Purpose

    For production of C-term flag-tagged TaGu R2 ORF mRNA for mRNA transfection in PRINT

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement mRNA
    

    Use

    Ivt template

    Tags

    3x-Flag

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT7-TaGu-ENT-3xF-ORF

    Plasmid

    #238488

    Purpose

    For production of C-term flag-tagged TaGu EN-tuned ORF mRNA for mRNA transfection in PRINT

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement mRNA
    

    Use

    Ivt template

    Tags

    3x-Flag

    Mutation

    R1109->A

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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