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pGL2_285

Plasmid

#251871

Purpose

Level 2 plasmid, barcoded ORI with erythromycin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2024.02.23.581749

Insert

pAL5000 ORI plus NGS barcode 1, erythromycin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pGL2_239

Plasmid

#251846

Purpose

Level 2 plasmid, barcoded ORI with chloramphenicol resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2024.02.23.581749

Insert

pAM_1 ORI plus NGS barcode 3, chloramphenicol resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pGL2_240

Plasmid

#251847

Purpose

Level 2 plasmid, barcoded ORI with chloramphenicol resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2024.02.23.581749

Insert

pBC1 ORI plus NGS barcode 25, chloramphenicol resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pGL2_241

Plasmid

#251848

Purpose

Level 2 plasmid, barcoded ORI with chloramphenicol resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2024.02.23.581749

Insert

pBP1 ORI plus NGS barcode 11, chloramphenicol resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pGL2_231

Plasmid

#251844

Purpose

Level 2 plasmid, barcoded ORI with chloramphenicol resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2024.02.23.581749

Insert

Dummy ORI plus NGS barcode 23, chloramphenicol resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pGL2_238

Plasmid

#251845

Purpose

Level 2 plasmid, barcoded ORI with chloramphenicol resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2024.02.23.581749

Insert

pUB110 ORI plus NGS barcode 2, chloramphenicol resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pGL2_300

Plasmid

#251885

Purpose

Level 2 plasmid, barcoded ORI with erythromycin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2024.02.23.581749

Insert

pUB110 ORI plus NGS barcode 2, erythromycin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pGL2_301

Plasmid

#251886

Purpose

Level 2 plasmid, barcoded ORI with erythromycin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2024.02.23.581749

Insert

pWKS1 ORI plus NGS barcode 17, erythromycin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pGL2_302

Plasmid

#251887

Purpose

Level 2 plasmid, barcoded ORI with erythromycin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2024.02.23.581749

Insert

RK2 ORI plus NGS barcode 12, erythromycin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pSS2 - pBait-1xMS2-7GC[empty]-TtrpA

Plasmid

#222406

Purpose

arabinose-induced synthesis of an RNA containing an MS2 hairpin, 7GC clamp and trpA terminator. Used as a three-hybrid control. For cloning purposes, see plasmid #222404.

Depositor

Katherine Berry

Article

Linh D. Nguyen et al Sci Rep 15, 3820 (2025)

Type

Empty backbone

Expression

Bacterial

Promoter

pBAD

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE-TurboID-HA-rtTA

Plasmid

#226995

Purpose

Backbone plasmid to express TurboID fusion proteins in mammalian cells

Depositor

Bruno Dallagiovanna

Article

Gomes-Junior et al PLoS One. 2025 May 30;20(5):e0324779. do

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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PlayBack-U6-Bamh1

Plasmid

#203298

Purpose

For expression of sgRNAs in a PlayBack Vector with Bamh1 as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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PlayBack-U6-Bgl11

Plasmid

#203299

Purpose

For expression of sgRNAs in a PlayBack Vector with BglII as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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PlayBack-U6-Not1

Plasmid

#203301

Purpose

For expression of sgRNAs in a PlayBack Vector with NotI as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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PlayBack-CMV-Nhe1

Plasmid

#203304

Purpose

For expression of eukaryotic genes of interest with CMV promoter with NheI as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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PlayBack-CMV-Bgl11

Plasmid

#203306

Purpose

For expression of eukaryotic genes of interest with CMV promoter with BglII as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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PlayBack-Ef1a-Nhe1

Plasmid

#203310

Purpose

For expression of eukaryotic genes of interest with Ef1a promoter with NheI as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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CAN1 gRNA HIS

Plasmid

#182521

Purpose

Expression vector for gRNA directed against CAN1 ORF.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

CAN1 gRNA (CAN1 Budding Yeast)

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT SMASh

Plasmid

#182523

Purpose

C-SWAT compatible vector for C-terminal tagging with SMASh Degron. (Protease Cleavage Site: DEMEECSKHL).

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

SMASh Tag

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT 3MYC SMASh

Plasmid

#182524

Purpose

C-SWAT compatible vector for C-terminal tagging with 3MYC followed by SMASh degron. (Protease Cleavage Site: DEMEECSKHL).

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

SMASh Tag

Tags

3MYC

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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