We narrowed to 2,328 results for: dap

1. pACT2-μ1B (137-423)

   Plasmid

   #198172

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-1 μ1B (137-423) fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

   Insert

   AP-1 μ1B (137-423)
   

   Use

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGBT9-AAGAB

   Plasmid

   #198332

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-AAGAB fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

   Insert

   AAGAB (AAGAB Human)
   

   Use

   Tags

   GAL4-DNA binding domain (BD) fragment

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBa.GFP-KIF3A tail 419-702

   Plasmid

   #193923

   Purpose

   Mammalian expression of GFP-tagged Kif 3A tail 419-702

   Depositor

   Marvin Bentley

   Article

   Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

   Insert

   Kif 3A (KIF3A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken Beta actin

   Available since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPR2_+10D+7U

   Plasmid

   #149393

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 7bp addition upstream of IHFprox site

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   7bp inserted upstream, and 10bp inserted downstre…

   Promoter

   pBAD

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPR2_+10D+10U

   Plasmid

   #149394

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 10bp addition upstream of IHFprox site

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   10bp inserted upstream and downstream of the IHFp…

   Promoter

   pBAD

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPR2_IHF_Mut

   Plasmid

   #149396

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with a mutated IHFprox site, that should not be recognized by IHF

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   IHFprox site is mutated at key positions needed f…

   Promoter

   pBAD

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFL GST-UBXN2A

   Plasmid

   #169016

   Purpose

   Expresses GST-UBXN2A (human) in insect cells

   Depositor

   Hemmo Meyer

   Article

   Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

   Insert

   UBXN2A (UBXN2A Human)
   

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin promoter

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMKO.1-shNuf2

   Plasmid

   #160961

   Purpose

   Nuf2 shRNA in pMKO.1 retroviral vector

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Nuf2 shRNA (Nuf2 Mouse)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. EcRfaHKOW-L1L2NusG

   Plasmid

   #113126

   Purpose

   Expresses C-terminus of E. coli RfaH with L1 and L2 loops replaced with NusG residues

   Depositor

   James Berger

   Article

   Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

   Insert

   RfaH (rfaH Escherichia coli)
   

   Use

   Tags

   His6-MBP-N10-TEV

   Expression

   Bacterial

   Mutation

   Deleted residues 1-107, replaced L1 and L2 loops …

   Promoter

   T7

   Available since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28P_dCrk

    Plasmid

    #131145

    Purpose

    Expresses 6XHis::dCrk (based on crk isoform RA).

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    Crk (Crk Fly)
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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