We narrowed to 4,714 results for: Drosophila

1. pFOSGE_COF2G02

   Plasmid

   #70876

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   Tip60 (Tip60 Fly)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pFOSGE_COF2G06

   Plasmid

   #70900

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   MED17 (MED17 Fly)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pFOSGE_COF2H07

   Plasmid

   #70908

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   CG7179 (CG7179 Fly)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pFOSGE_COF2H09

   Plasmid

   #70924

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   Mnn1 (Mnn1 Fly)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pAS-2 (mm21)

   Plasmid

   #40837

   Purpose

   Target RNAs used to determine complementarity requirements for in vitro tailing and degradation

   Depositor

   Phillip Zamore

   Article

   Ameres et al Science. 2010 Jun 18;328(5985):1534-9.

   Insert

   let-7–mm21
   

   Use

   Mirna reporter

   Tags

   EGFP

   Expression

   Insect

   Mutation

   Promoter

   Actin5C

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pAS-13

   Plasmid

   #40848

   Purpose

   Target RNAs used to determine complementarity requirements for in vitro tailing and degradation

   Depositor

   Phillip Zamore

   Article

   Ameres et al Science. 2010 Jun 18;328(5985):1534-9.

   Insert

   let-7–Bartel
   

   Use

   Mirna reporter

   Tags

   EGFP

   Expression

   Insect

   Mutation

   Promoter

   Actin5C

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

7. XE92 XGCNF-EnR

   Plasmid

   #17029

   Depositor

   Randall Moon

   Article

   Song et al Dev Biol. 1999 Sep 1;213(1):170-9. doi:

   Insert

   XGCNF-EnR (en Fly)
   

   Use

   Xenopus expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pDam-Myc-HP1

   Plasmid

   #59221

   Purpose

   expresses Dam-myc-HP1

   Depositor

   Bas van Steensel

   Article

   van Steensel et al Nat Biotechnol. 2000 Apr;18(4):424-8.

   Insert

   HP1 (Su(var)205 Fly)
   

   Use

   Damid

   Tags

   Dam and myc

   Expression

   Insect

   Mutation

   Promoter

   Drosophila heat shock promoter

   Available since

   May 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET45b-tsr(B/X) (6X-His-Dm-Cofilin)

   Plasmid

   #128278

   Purpose

   Expresses Drosophila melanogaster Cofilin (Twinstar) in bacterial cell culture

   Depositor

   Anna Sokac

   Article

   Figard et al Cell Rep. 2019 Mar 26;26(13):3493-3501.e

   Insert

   twinstar (tsr) (tsr Fly)
   

   Use

   Tags

   6X Histidine tag

   Expression

   Bacterial

   Mutation

   Met1 changed to Pro1; Cys77 silent basepair mutat…

   Promoter

   T7

   Available since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUb FLAG-Mts D85N

    Plasmid

    #208403

    Purpose

    Expresses FLAG-tagged Drosophila mts protein with D85N mutation

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    microtubule star (mts Fly)
    

    Use

    Tags

    3xFLAG

    Expression

    Insect

    Mutation

    D85N

    Promoter

    Ubi-p63e

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUb FLAG-Mts H59Q

    Plasmid

    #208402

    Purpose

    Expresses FLAG-tagged Drosophila mts protein with H59Q mutation

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    microtubule star (mts Fly)
    

    Use

    Tags

    3xFLAG

    Expression

    Insect

    Mutation

    H59Q

    Promoter

    Ubi-p63e

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUb FLAG-Mts H241A

    Plasmid

    #208404

    Purpose

    Expresses FLAG-tagged Drosophila mts protein with H241A mutation

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    microtubule star (mts Fly)
    

    Use

    Tags

    3xFLAG

    Expression

    Insect

    Mutation

    H241A

    Promoter

    Ubi-p63e

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUb FLAG-Mts

    Plasmid

    #195065

    Purpose

    Expresses FLAG-tagged Drosophila mts protein

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    microtubule star (mts Fly)
    

    Use

    Tags

    3xFLAG

    Expression

    Insect

    Mutation

    Promoter

    Ubi-p63e

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pnEA-vH_His-TEV-DSep1-msfGFP

    Plasmid

    #174494

    Purpose

    bacterial expression of Drosophila Sep1 fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Insert

    Sep1 (Sep1 Fly)
    

    Use

    Tags

    His6-TEV and msfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHRW-CRY2-VHH(GFP)

    Plasmid

    #126022

    Purpose

    express RFP-tagged CRY2 PHR domain fused to anti-GFP nanobody for optogenetics

    Depositor

    Eurico Morais-de-Sá

    Article

    Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

    Insert

    CRY2 PHR-VHH(GFP)
    

    Use

    Tags

    RFP

    Expression

    Insect

    Mutation

    WT

    Promoter

    Hsp70

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. Ac5-STABLE2-puro

    Plasmid

    #208032

    Purpose

    Enables expression of 2 ORFs (with or without tags) and selection with puromycin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-puro with Actin5C promoter.

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJAT71

    Plasmid

    #204305

    Purpose

    Expresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable ie1XP3-DsRed cassette expressed in abdomen and mouthparts.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    ie1-DsRed
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ie1

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSEED-sfGFP

    Plasmid

    #219639

    Purpose

    Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    sfGFP SEED Cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 phiC31

    Plasmid

    #68310

    Purpose

    In vitro transcription vector to generate phiC31 mRNA (i.e. for zebrafish injection).

    Depositor

    Konrad Basler

    Article

    Bischof et al Proc Natl Acad Sci U S A. 2007 Feb 27;10

    Insert

    phiC31
    

    Use

    Rna transcription

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUb PspCas13b + PspCas13b guide RNA

    Plasmid

    #176305

    Purpose

    Expresses PspCas13b and its associated guide RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

    Insert

    PspCas13b
    

    Use

    Tags

    HA tag

    Expression

    Insect

    Mutation

    E113K, N265S- please depositor comments

    Promoter

    Ubi-p63e

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge