We narrowed to 13,608 results for: crispr cas9

1. pSpCas9-Puro HLAG-2A-sgRNA

   Plasmid

   #182551

   Purpose

   Cas9 from S.pyogenes with 2A-Puro, and the 2A-sgRNA to targeting exon 2 of human HLA-G gene

   Depositor

   Santiago Miriuka

   Article

   Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

   Insert

   hSpCas9-2A-Puro-HLAG-2A-sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. SIN-PGK-Cas9-V5-WPRE

   Plasmid

   #87876

   Purpose

   To produce lentiviral vector for Cas9 editing

   Depositor

   Nicole Déglon

   Article

   Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

   Insert

   spCas9
   

   Use

   Lentiviral

   Tags

   V5

   Mutation

   Human codon-optimized

   Promoter

   mouse PGK

   Available Since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9-Puro HLAG-2B-sgRNA

   Plasmid

   #182552

   Purpose

   Cas9 from S.pyogenes with 2A-Puro, and the 2B-sgRNA to targeting exon 2 of human HLA-G gene

   Depositor

   Santiago Miriuka

   Article

   Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

   Insert

   hSpCas9-2A-Puro-HLAG-2B-sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. SIN-CMV-Cas9-V5-WPRE

   Plasmid

   #87904

   Purpose

   To produce lentiviral vector for gene editing

   Depositor

   Nicole Déglon

   Article

   Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

   Insert

   Cas9-V5
   

   Use

   Lentiviral

   Tags

   V5

   Mutation

   human codon-optimized

   Promoter

   CMV

   Available Since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. lenti-Cas9-VQR-GFP_activity_reporter

   Plasmid

   #87156

   Purpose

   This lentiviral vector can be used to assay activity of SpCas9-VQR (NGA PAM restricted).

   Depositor

   Daniel Bauer

   Article

   Canver et al Nat Genet. 2017 Feb 20. doi: 10.1038/ng.

   Insert

   GFP and sgRNA targeting GFP (NGA restricted)
   

   Use

   Lentiviral

   Available Since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. SIN-PGK-Cas9-WPRE

   Plasmid

   #87886

   Purpose

   To produce Lentiviral vector for gene editing

   Depositor

   Nicole Déglon

   Article

   Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

   Insert

   hCas9
   

   Use

   Lentiviral

   Tags

   nls

   Mutation

   Human codon-optimized

   Promoter

   mouse PGK

   Available Since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpCas9(BB)-2A-puro-hANKRD11 (exon 5-2)

   Plasmid

   #236613

   Purpose

   Knockouts ANKRD11 in human cells

   Depositor

   Tadashi Nakagawa

   Article

   Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

   Insert

   ANKRD11 (ANKRD11 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Sept. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9(BB)-2A-puro-hANKRD11 (exon 5-1)

   Plasmid

   #236612

   Purpose

   Knockouts ANKRD11 in human cells

   Depositor

   Tadashi Nakagawa

   Article

   Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

   Insert

   ANKRD11 (ANKRD11 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   June 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9(BB)-2A-puro-mAnkrd11 (intron 3)

   Plasmid

   #236608

   Purpose

   Knockouts Ankrd11 in mouse cells

   Depositor

   Tadashi Nakagawa

   Article

   Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

   Insert

   Ankrd11 (Ankrd11 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   June 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. AID-C12*-n'Cas9-BE4max

    Plasmid

    #216730

    Purpose

    Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and n'Cas9 (Cas9 with H840A mutation) in mammalian cells.

    Depositor

    Rahul Kohli

    Article

    Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

    Insert

    AID-C12*-n'Cas9-BE4max (AICDA Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. AID-C12*-dCas9-BE4max

    Plasmid

    #216731

    Purpose

    Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and dCas9 (Cas9 with D10A and H840A mutations) in mammalian cells.

    Depositor

    Rahul Kohli

    Article

    Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

    Insert

    AID-C12*-dCas9-BE4max (AICDA Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAVss-U6-sgHTT51-7sk-Cas9

    Plasmid

    #190901

    Purpose

    AAV-KamiCas9 vector expressing sgGFP and mouse HTT sgRNA

    Depositor

    Nicole Déglon

    Article

    Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

    Insert

    sgRNA (Htt Mouse)
    

    Use

    AAV and CRISPR

    Promoter

    U6 and 7sk

    Available Since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-T7-ABE8e-SpCas9-NRTH-P2A-EGFP (RMD21)

    Plasmid

    #197503

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpCas9-NRTH-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-NRTH(D10A/I32…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV:ITR-EF1aCore-SaCas9-dual(U6-sgRNA(backbone))-ITR

    Plasmid

    #207878

    Purpose

    AAV vector encoding EF1a core promoter-driven SaCas9 and two sgRNA cloning sites w/ the engineered Sa Guide scaffold variant (BbsI and PaqCI for sgRNA cloning).

    Depositor

    Yeh-Hsing Lao

    Article

    Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV:ITR-EF1aCore-SaCas9-single(U6-sgRNA(backbone))-ITR

    Plasmid

    #207880

    Purpose

    AAV vector encoding EF1a core promoter-driven SaCas9 and single sgRNA cloning site w/ the engineered Sa Guide scaffold variant (BbsI for sgRNA cloning).

    Depositor

    Yeh-Hsing Lao

    Article

    Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-T7-ABE8e-SpCas9-NRRH-P2A-EGFP (RMD51)

    Plasmid

    #197502

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpCas9-NRRH-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-NRRH(D10A/I32…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-T7-ABE8.20m-SpCas9-NRCH-P2A-EGFP (RMD55)

    Plasmid

    #197501

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRCH-BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8.20 mutations in TadA and nSpCas9-NRCH(D10A/I…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV-pCBh-ABE8e-nSpCas9(D10A)[N-term] (HES1468)

    Plasmid

    #197509

    Purpose

    Cbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A)

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    AAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A)[N-term]-NpuN-BPNLS-[ITR]
    

    Use

    AAV and CRISPR

    Tags

    BPNLS and NpuN(intein)-BPNLS

    Mutation

    ABE8e mutations in TadA and nSpCas9(D10A)

    Promoter

    Cbh

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-T7-ABE8.20m-SpCas9-NRRH-P2A-EGFP (RMD47)

    Plasmid

    #197499

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRRH-BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8.20 mutations in TadA and nSpCas9-NRRH(D10A/I…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-T7-ABE8e-SpCas9-NRCH-P2A-EGFP (RMD57)

    Plasmid

    #197504

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-ABE8e-NRCH-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-NRCH(D10A/I32…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge