We narrowed to 10,998 results for: ENA;

1. pPW3757 : CMVd3-ERmembrane-2xHaloTag-KKMP

   Plasmid

   #185679

   Purpose

   Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane.

   Depositor

   Peter Walter

   Article

   Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

   Insert

   ERmembrane-2xHaloTag-KKMP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSilencer2.1-U6-T1-U6-bc339

   Plasmid

   #190849

   Purpose

   For mammalian expression of T1 and bc339, the RNA aptamers of ncOGT and β-catenin, respectively. Expression of each aptamer is driven by a U6 promoter.

   Depositor

   Gerald Hart

   Article

   Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

   Inserts

   T1 (an RNA aptamer of O-GlcNAc Transferase)
   bc339 (an RNA aptamer of β-catenin)
   

   Use

   Affinity Reagent/ Antibody and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Jan. 9, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pLY227

   Plasmid

   #184148

   Purpose

   short crRNA generator with spacer LEA2 with WT repeat region

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

   Insert

   s-crRNA-LEA2-WT
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Plux2

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLY232

   Plasmid

   #184149

   Purpose

   short tracrRNA generator with WT anti-repeat region

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

   Insert

   s-tracrRNA-WT
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD promoter

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLY171

   Plasmid

   #184145

   Purpose

   sgRNA with 3 RNA aptemers BoxB at the 3'-end for CRISPRa

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

   Insert

   sgRNA-LEA2-ex3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Plux2

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pYPQ131-RZ-Cas12j2

   Plasmid

   #189782

   Purpose

   Golden Gate entry vector to clone the 1st Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

   Depositor

   Yiping Qi

   Article

   Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pYPQ132-RZ-Cas12j2

   Plasmid

   #189783

   Purpose

   Golden Gate entry vector to clone the 2nd Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

   Depositor

   Yiping Qi

   Article

   Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pYPQ134-RZ-Cas12j2

   Plasmid

   #189785

   Purpose

   Golden Gate entry vector to clone the 4th Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

   Depositor

   Yiping Qi

   Article

   Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pYPQ131-RZ-Cas12j2

   Plasmid

   #173927

   Purpose

   Golden Gate entry vector; empty vector to clone the 1st Cas12j2 crRNA with ribozyme processing

   Depositor

   Yiping Qi

   Article

   Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYPQ132-RZ-Cas12j2

    Plasmid

    #173928

    Purpose

    Golden Gate entry vector; empty vector to clone the 2nd Cas12j2 crRNA with ribozyme processing

    Depositor

    Yiping Qi

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pYPQ133-RZ-Cas12j2

    Plasmid

    #173929

    Purpose

    Golden Gate entry vector; empty vector to clone the 3rd Cas12j2 crRNA with ribozyme processing

    Depositor

    Yiping Qi

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pYPQ134-RZ-Cas12j2

    Plasmid

    #173930

    Purpose

    Golden Gate entry vector; empty vector to clone the 4th Cas12j2 crRNA with ribozyme processing

    Depositor

    Yiping Qi

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSEVA251::PpsbA2*::B0032::CYP110D1

    Plasmid

    #186707

    Purpose

    CYP110D1 coding sequence under the control of PpsbA2* promoter and the BBa_B0032 RBS, for the expression in Synechocystis.

    Depositor

    Paula Tamagnini

    Article

    Mascia et al Green Chemistry, 24 (2022):6156

    Insert

    alr4766
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PpsbA2*

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSEVA251::Ptrc.x.tetO2::B0032::CYP110D1

    Plasmid

    #186709

    Purpose

    CYP110D1 coding sequence under the control of Ptrc.x.tetO2 promoter and the BBa_B0032 RBS, for the expression in Synechocystis.

    Depositor

    Paula Tamagnini

    Article

    Mascia et al Green Chemistry, 24 (2022):6156

    Insert

    alr4766
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Ptrc.x.tetO2

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOD_004

    Plasmid

    #155361

    Purpose

    Gentamycin version of the scarless genome engineering pDEL plasmid (Tikh et al. 2016) compatible with Salmonella

    Depositor

    Elisabeth Raleigh

    Article

    Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 30, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pOD_003

    Plasmid

    #155362

    Purpose

    Zeomycin version of the scarless genome engineering pDEL plasmid (Tikh et al. 2016) compatible with Salmonella

    Depositor

    Elisabeth Raleigh

    Article

    Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 30, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pT-GFP-rG1

    Plasmid

    #188967

    Purpose

    IPTG inducible GFP with sgRNA

    Depositor

    Seung-Goo Lee

    Article

    Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

    Inserts

    GFP
    sgRNA: agtggaaaacaatgcgaccgactagt
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptrc

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pT-GFP-rG2

    Plasmid

    #188968

    Purpose

    IPTG inducible GFP with sgRNA

    Depositor

    Seung-Goo Lee

    Article

    Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

    Inserts

    GFP
    sgRNA: agtccatgtaatcagcgtctactagt
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptrc

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBA1031

    Plasmid

    #189584

    Purpose

    EdH4gp0214 deletion HR vector 250bp HR Arms (EdH4 phage editing)

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Insert

    EdH4gp0214 Deletion Locus 250bp Homology Arms
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Encodes for a full deletion of EdH4gp0214

    Promoter

    Available Since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBA1030

    Plasmid

    #189582

    Purpose

    EdH4gp0004 deletion HR vector 250bp HR Arms (EdH4 phage editing)

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Insert

    EdH4gp0004 Deletion Locus 250bp Homology Arms
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Encodes for a full deletion of EdH4gp0004

    Promoter

    Available Since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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