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We narrowed to 13,167 results for: Mpl;

Showing: 5721 - 5760 of 13167 results

  4. pMBEC6

    Plasmid
    #187598
    Purpose
    Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Gentamycin resistance
    Depositor
    Pablo Ivan Nikel
    Article
    Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:
    Insert
    nCas9-BEC-UGI; cas6f, gfp
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    trc
    Available since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    187598-plasmid-map-sequence-id-367542

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  5. pTS101 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-SUMOEu-MCS

    Plasmid
    #199354
    Purpose
    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-SUMOEu-tagged protein in mammalian cells.
    Depositor
    Rebecca Voorhees
    Article
    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    TagBFP-P2A-ALFA-SUMOEu
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    199354-plasmid-map-sequence-id-389904

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  8. qTAG-N-Solo-mStayGold-TJP1

    Plasmid
    #227300
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the TJP1 locus. For tight junction visualization. To be co-transfected with sgRNA plasmid px330-TJP1 (Addgene #227299)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TJP1 Homology Arms flanking a mStayGold Tag (TJP1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227300-plasmid-map-sequence-id-451538

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  9. pT040

    Plasmid
    #67640
    Purpose
    URA3 vector for cloning guide sequences using BclI-SwaI sites into guide RNA expression cassette
    Depositor
    John Wyrick
    Article
    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1
    Insert
    single guide RNA expression cassette
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pSNR52
    Available since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67640-plasmid-map-sequence-id-120917

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  15. pNLS-2xBFP(4C)

    Plasmid
    #176151
    Purpose
    Encodes NLS-2xBFP(4C), which is a tandem BFP with an N-terminal SV40 NLS. The protein has 4 solvent accessible cysteines for labeling with maleimide dyes.
    Depositor
    Siegfried Musser
    Article
    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d
    Insert
    NLS-2xBFP(4C)
    Use
    Tags
    6xHis tag and SV40 nuclear localization sequence …
    Expression
    Bacterial
    Mutation
    Compared with EGFP, the two BFP domains have the …
    Promoter
    Ptrc
    Available since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    176151-plasmid-map-sequence-id-356068

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  17. pJRH051

    Plasmid
    #171625
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-BsmBI-Destination
    mNeon
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-a and U6
    Available since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    171625-plasmid-map-sequence-id-335505

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  19. pENTR_L1-YFP-miniTurbo-NES-L2

    Plasmid
    #127351
    Purpose
    gateway entry vector for making N-terminal YFP-miniTurbo fusions with non-nuclear proteins
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    miniTurbo (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, NES, V5, and YFP
    Expression
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127351-plasmid-map-sequence-id-246289

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  26. pAf-CRISPR-yA

    Plasmid
    #191015
    Purpose
    CRISPR vector based on Aspergillus flavus U6 promoter and terminator for targeting the yA gene, which contains AMA1(the HindIII-PstI fragment) and ptrA selection marker.
    Depositor
    Perng-Kuang Chang
    Article
    Chang et al Microbiol Spectr. 2023 Feb 14;11(1):e046
    Insert
    yA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Aspergillus flavus U6
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    191015-plasmid-map-sequence-id-382132

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  29. pENTR_L1-YFP-miniTurbo-L2

    Plasmid
    #127352
    Purpose
    gateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteins
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    miniTurbo (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, V5, and YFP
    Expression
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127352-plasmid-map-sequence-id-246314

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  34. pMBEC8

    Plasmid
    #187599
    Purpose
    Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Apramycin resistance
    Depositor
    Pablo Ivan Nikel
    Article
    Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:
    Insert
    nCas9-BEC-UGI; cas6f, gfp
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    trc
    Available since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    187599-plasmid-map-sequence-id-367572

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Showing: 5721 - 5760 of 13167 results