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We narrowed to 3,637 results for: golden gate

Showing: 2861 - 2880 of 3637 results

  2. Bxb1-GT_hEF1a-mGL_CAG-mRuby2_Divergent

    Plasmid
    #229800
    Purpose
    Bxb1-GT donor plasmid with divergent syntax for constitutively expressing mRuby2 and mGreenLantern by the respective promoters CAG and hEF1a
    Depositor
    Richard Davis
    Article
    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.
    Insert
    mGreenLantern; mRuby2
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hEF1a and CAG
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    229800-plasmid-map-sequence-id-461681

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  3. pHW1643

    Plasmid
    #229876
    Purpose
    gene trap RMCE plasmid with Tet-On[rTetR-linker-QFAD]-act-4 3' UTR::SEC cloned in the destination vector pHW1133 to swap driver via RMCE
    Depositor
    Han Wang
    Article
    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.
    Insert
    Tet-On[rTetR-linker-QFAD]-act-4 3' UTR::SEC
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    229876-plasmid-map-sequence-id-460844

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  4. pHW1221

    Plasmid
    #229873
    Purpose
    gene trap RMCE plasmid with QF-act-4 3' UTR::SEC for RMCE cloned in the destination vector pHW1133 to swap driver via RMCE
    Depositor
    Han Wang
    Article
    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.
    Insert
    QF-act-4 3' UTR::SEC
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    229873-plasmid-map-sequence-id-460840

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  5. pHW1177

    Plasmid
    #229872
    Purpose
    gene trap RMCE plasmid with Tet-Off[TetR-linker-QFAD]-act-4 3'UTR::SEC cloned in the destination vector pHW1133 to swap driver via RMCE
    Depositor
    Han Wang
    Article
    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.
    Insert
    Tet-Off[TetR-QFAD]-act-4 3'UTR::SEC
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    229872-plasmid-map-sequence-id-460839

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  7. pTBL3328 PB-sfGFP-WPRE-target

    Plasmid
    #226676
    Purpose
    PiggyBac cargo vector encoding sfGFP followed by WPRE and a transcribed peCHYRON target site.
    Depositor
    Chang Liu
    Article
    Loveless et al bioRxiv 2021.11.05.467507
    Insert
    sfGFP-WPRE-site3target
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1alpha
    Available since
    Dec. 17, 2024
    Availability
    Academic Institutions and Nonprofits only

  8. CROPseq-Puro-2xMS2

    Plasmid
    #212627
    Purpose
    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles
    Depositor
    Paul Blainey
    Article
    Najia et al bioRxiv 2024.10.01.616098
    Insert
    EF1a-PuroR-2xMS2-WPRE-hU6-sgRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    212627-plasmid-map-sequence-id-420278

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  9. CROPseq-Puro-12xMS2

    Plasmid
    #212628
    Purpose
    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles
    Depositor
    Paul Blainey
    Article
    Najia et al bioRxiv 2024.10.01.616098
    Insert
    EF1a-PuroR-12xMS2-WPRE-hU6-sgRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    212628-plasmid-map-sequence-id-422312

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  10. CROPseq-Puro-4xMS2

    Plasmid
    #212626
    Purpose
    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles
    Depositor
    Paul Blainey
    Article
    Najia et al bioRxiv 2024.10.01.616098
    Insert
    EF1a-PuroR-4xMS2-WPRE-hU6-sgRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    212626-plasmid-map-sequence-id-420274

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  12. pJB53

    Plasmid
    #226309
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    CsoS2 with V(T/S)G to AAA CsoS2 MR1-3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    VTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…
    Promoter
    pTRC
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226309-plasmid-map-sequence-id-449921

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  13. pJJB1559

    Plasmid
    #218590
    Purpose
    Express peroxisomal Idi1p, Erg20(F96W,N127W)p, and GESp to convert IPP to geraniol with cytosolic Erg20 homodimer
    Depositor
    John Dueber
    Article
    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038
    Insert
    GES
    Use
    Tags
    ePTS1
    Expression
    Yeast
    Mutation
    Erg20(F96W, N127W), Erg20-Erg20 synthetic homodim…
    Promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    218590-plasmid-map-sequence-id-432609

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  16. TL1 alphaDdCBE T2 (DddA6-N)

    Plasmid
    #221995
    Purpose
    Expresses mitochondrial cytosine base editor arm in mammalian cells
    Depositor
    Stephen Ekker
    Article
    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089
    Insert
    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE T2 repeats-TALE CTD-DddA6 1397N-UGI-ATP5B 3'UTR
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    TALE: Q231R, W232G, S233A | DddA: Q1310R, S1330I,…
    Promoter
    CMV
    Available since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    221995-plasmid-map-sequence-id-438696

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  17. ATP6 alphaDdCBE G2

    Plasmid
    #221959
    Purpose
    Expresses mitochondrial cytosine base editor arm in mammalian cells
    Depositor
    Stephen Ekker
    Article
    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089
    Insert
    COX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE G2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    TALE: Q231R, W232G, S233A, See depositor comments
    Promoter
    CMV
    Available since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    221959-plasmid-map-sequence-id-438714

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Showing: 2861 - 2880 of 3637 results