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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,523 results for: golden gate

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Showing: 2861 - 2880 of 5523 results

  1. pML325_GPR107-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227909
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Publication
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPR107 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    Use
    CRISPR
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227909-plasmid-map-sequence-id-452501

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  2. pML359_CAPRIN1-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227916
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Publication
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CAPRIN1 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    Use
    CRISPR
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227916-plasmid-map-sequence-id-456019

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  3. pML346_VPS35-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227891
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Publication
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VPS35 homology arms with mEGFP-3xHA for insertion at C terminus
    Use
    CRISPR
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227891-plasmid-map-sequence-id-455737

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  6. pML348_ATG101-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227900
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Publication
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATG101 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    Use
    CRISPR
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227900-plasmid-map-sequence-id-455749

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  7. pML460_MAP1LC3B-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227901
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Publication
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MAP1LC3B homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    Use
    CRISPR
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227901-plasmid-map-sequence-id-457382

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  8. pML317_COPE-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227905
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Publication
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    COPE homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    Use
    CRISPR
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227905-plasmid-map-sequence-id-456011

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  12. pVP077

    Plasmid
    #222029
    Purpose
    Empty Kanamycin resistant GreenGate destination vector based on pGGP-AG. Domesticated of Esp3I sites in the backbone and chloramphenicol resistance gene.
    Depositor
    Wayne Parrott
    Publication
    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do
    Type
    Empty backbone
    Use
    Synthetic Biology; Greengate compatible cloning v…
    Mutation
    All Esp3i sites domesticated
    Available since
    Dec. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    222029-plasmid-map-sequence-id-447312

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  18. pR-mTagBFP2

    Plasmid
    #228596
    Purpose
    Mammalian expression of mTagBFP2, for MuSIC barcode construction
    Depositor
    Marc Birtwistle
    Publication
    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mTagBFP2
    Expression
    Mammalian
    Mutation
    We introduced some silent mutations to avoid esse…
    Promoter
    CMV
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    228596-plasmid-map-sequence-id-454228

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Showing: 2861 - 2880 of 5523 results