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pNK2657

Plasmid

#219741

Purpose

MoClo-compatible Level 0 promoterless vector encoding hispidin-synthase from Mycena citricolor codon-optimised for expression in Nicotiana benthamiana, Pichia pastoris, Homo sapiens

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

hispidin- synthase from Mycena citricolor

Use

Synthetic Biology

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pEXP5-aHL_spytag_his

Plasmid

#107334

Purpose

Monomeric SpyTag-6×-His alpha-hemolysin

Depositor

George Church

Article

Stranges et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

alpha-hemolysin-SpyTag-6xHis

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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anti-GFP scFv [N86/38] with sortase tag

Plasmid

#204419

Purpose

Mammalian Expression of anti-GFP scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N86/38.

Depositor

Jeff Lichtman

Article

Han et al Nat Commun. 2024 Aug 5;15(1):6648. doi:

Insert

Recombinant mouse scFv targeting GFP (Aequorea victoria)

Tags

6xHis tag and Sortase tag

Expression

Mammalian

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pX026

Plasmid

#167153

Purpose

MoClo-compatible Level 1 (position 4) vector encoding Neonothopanus nambi luciferase nnLuz with C-terminal His-tag under control of 0.4 kb 35S promoter, for expression in plants

Depositor

Karen Sarkisyan

Article

Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

Insert

p35s-nnLuz-ocsT

Use

Luciferase

Tags

His6-tag

Expression

Plant

Promoter

p35s

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Flag-Med25

Plasmid

#64771

Purpose

Mammalian protein expression of flag-tagged Med25

Depositor

Joan Conaway, Ronald Conaway

Article

Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

Insert

Med25 (MED25 Human)

Tags

Flag

Expression

Mammalian

Promoter

pCMV

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
mCRY1 HA tag

Plasmid

#110298

Purpose

full length wt mouse Cry1 HA tag in pcDNA3.1

Depositor

Joseph Takahashi

Article

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Insert

wt mcry1 HA tag (Cry1 Mouse)

Tags

HA tag

Expression

Mammalian

Mutation

wildtype

Promoter

CMV

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pmCherryC1 VAP-A KD/MD

Plasmid

#226408

Purpose

Expression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to mCherry in mammalian cells

Depositor

Catherine Tomasetto

Article

Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

Insert

VAP-A (VAPA Human)

Tags

T7-Xpress tag and mCherry

Expression

Mammalian

Mutation

K94D/M96D mutant, unable to bind FFAT motifs

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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Pancortin scFv [K96/7]

Plasmid

#190497

Purpose

Mammalian Expression of Pancortin scFV. Derived from hybridoma K96/7.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Pancortin (Homo sapiens) recombinant scFV (OLFM1 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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VGlut2 scFv [N29/29]

Plasmid

#182085

Purpose

Mammalian Expression of VGlut2 scFv. Derived from hybridoma N29/29.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting VGlut2 (Rattus norvegicus) (Slc17a6 Mouse)

Tags

HA, His

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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TrpC5 scFv [N67/15]

Plasmid

#190564

Purpose

Mammalian Expression of TrpC5 scFV. Derived from hybridoma N67/15.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

TrpC5 (Homo sapiens) recombinant scFV (TRPC5 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Bcl2-Maob

Plasmid

#18001

Depositor

Clark Distelhorst

Article

Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

Insert

Mitochondria-targeted Bcl-2 (BCL2 Human)

Tags

GFP

Expression

Mammalian

Mutation

Bcl-2's C terminal replaced with mitochondri…

Promoter

CMV

Available Since

June 4, 2008

Availability

Academic Institutions and Nonprofits only
Tom20-mChF-AIP

Plasmid

#61512

Purpose

Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIP

Depositor

Takanari Inoue

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

FK506 binding protein 1A (FKBP1A Human)

Tags

AIP, Tom20, and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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Tom20-mChF-AIP(TA)

Plasmid

#61513

Purpose

Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIP with TA mutation

Depositor

Takanari Inoue

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

FK506 binding protein 1A (FKBP1A Human)

Tags

AIP with TA mutation (see comments), Tom20, and m…

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
SPHKAP scFv [L131/17]

Plasmid

#190511

Purpose

Mammalian Expression of SPHKAP scFV. Derived from hybridoma L131/17.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

SPHKAP (Mus musculus) recombinant scFV (Sphkap Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Parvalbumin scFv [L114/3]

Plasmid

#190504

Purpose

Mammalian Expression of Parvalbumin scFV. Derived from hybridoma L114/3.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Parvalbumin (Rattus norvegicus) recombinant scFV (Pvalb Mouse)

Tags

HA, 6xHis, Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pan-Synapsin scFv [L125/129]

Plasmid

#182079

Purpose

Mammalian Expression of pan-Synapsin scFv. Derived from hybridoma L125/129.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting pan-Synapsin

Tags

Sortase, His

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pPW3755 : HDR-HsIRE1a-10xGS-HaloTag

Plasmid

#185677

Purpose

HDR template used for endogenous CRISPR editing of ERN1 gene, inserting a C-terminal HaloTag.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERN1 homology arms (ERN1 Human, Synthetic)

Use

CRISPR

Tags

HaloTag

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pQCXIP mScarlet-MOSPD2 RD/LD

Plasmid

#186476

Purpose

Expression of human MOSPD2 (mutant R404D/L406D, unable to bind FFAT motifs) fused to mScarlet in mammalian cells

Depositor

Catherine Tomasetto

Article

Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Use

Retroviral

Tags

mScarlet

Expression

Mammalian

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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HA-HIF2α P405A/P531A-pBabe-Hygro

Plasmid

#21676

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

cDNA changed amino acids from wt: Proline 405 t…

Available Since

Sept. 4, 2009

Availability

Academic Institutions and Nonprofits only

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FLINC-AKAR1-CAAX

Plasmid

#87705

Purpose

Fluorescent biosensor for super-resolution imaging of PKA activity; targeted to plasma membrane.

Depositor

Jin Zhang

Article

Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

Insert

FLINC-AKAR1-CAAX

Tags

C-terminal targeting sequence from Kras

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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