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DNA-PKcs ABCDE>A

Plasmid

#83318

Purpose

human DNA-PKcs/ABCDE>ala

Depositor

Katheryn Meek

Article

Cui et al Mol Cell Biol. 2005 Dec;25(24):10842-52.

Insert

human DNA-PKcs (PRKDC Human)

Expression

Mammalian

Mutation

6 phosphorylation sites in the ABCDE cluster subs…

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pWZL Blast Snail ER

Plasmid

#18798

Depositor

Bob Weinberg

Article

Mani et al Cell. 2008 May 16. 133(4):704-15.

Insert

snail (SNAI1 Human)

Use

Retroviral

Tags

ER hormone binding domain

Expression

Mammalian

Mutation

G525R mutation in the ER. The presence of the G52…

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV_GFAP_HyPer7_mito

Plasmid

#238966

Purpose

Mammalian astrocytes HyPer7 expression targeted to the mitochondrial matrix

Depositor

Dmitry Bilan

Article

Kotova et al Free Radic Biol Med. 2023 Nov 1;208:153-

Insert

HyPer7

Use

AAV

Tags

3x Cytochrome C Oxidase Subunit VIII presequense

Expression

Mammalian

Promoter

GFAP

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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acta2_mCherry_pA_pDestTolpA2

Plasmid

#78685

Purpose

acta2:mCherry transgenesis construct

Depositor

Sarah Childs

Article

Whitesell et al PLoS One. 2014 Mar 3;9(3):e90590. doi: 1

Insert

acta2 promoter (acta2 Zebrafish)

Use

Unspecified

Tags

mCherry

Promoter

acta2

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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acta2_GFP_pA_pDestTolpA2

Plasmid

#78684

Purpose

acta2:GFP transgenesis construct

Depositor

Sarah Childs

Article

Whitesell et al PLoS One. 2014 Mar 3;9(3):e90590. doi: 1

Insert

acta2 promoter (acta2 Zebrafish)

Use

Unspecified

Promoter

acta2

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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Triple ORF LoxP ifgMosaic Donor (LH208)

Plasmid

#99625

Purpose

To clone the 3 ORFs for Cre dependent generation of ifgMosaics

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

N-PhiM

Use

Cre/Lox, Mouse Targeting, and Synthetic Biology

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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LoxP1-His-H2B-Cherry-2A (SO290)

Plasmid

#99616

Purpose

To clone gene of interest downstream of LoxP1-His-H2B-Cherry-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

6xHis-H2B-Cherry

Tags

T2A

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pICE-HA-CtIP-siR-S664A-S679A-S745A

Plasmid

#82031

Purpose

Plasmid for constitutive or doxy-inducible expression of S664A+S679A+S745A human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Sébastien Britton, Patrick Calsou

Article

Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

Insert

CtIP (RBBP8 Human)

Tags

HA

Expression

Mammalian

Mutation

Non phosphorylatable mutations on 3 of the main A…

Promoter

CMV-tet

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pGal-Trans-Myc-HA-GFP11-KDEL

Plasmid

#177724

Purpose

ER protein collagen beta (1-O) galactosyltransferase with a C-terminal Myc tag, an HA tag and the eleventh β-strand of GFP

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

collagen beta(1-O)galactosyltransferase 1 (COLGALT1 Human)

Tags

GFP11, HA, KDEL, and Myc

Expression

Bacterial and Mammalian

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pGEM BirA-2A-Citrine-SV40pA-FRT-Kan-FRT

Plasmid

#89890

Purpose

BAC donor construct containing 3XHA-tagged BirA, a ribosome skipping motif - 2A, Citrine reporter, polyadenyation signal, followed by FRT recombination sites flanking kanamycin selection cassette.

Depositor

Tatjana Sauka-Spengler

Article

Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

Insert

HA-BirA-2A-citrine

Use

Unspecified

Tags

Citrine

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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FRT1-His-H2B-Cherry-2A (IG156)

Plasmid

#99622

Purpose

To clone gene of interest downstream of FRT1-His-H2B-Cherry-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

His-H2B-Cherry

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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4xnr UAS ifgMosaic Donor vector (IG230)

Plasmid

#99631

Purpose

To clone the 3 ORFs for Gal4 dependent generation of ifgMosaics

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

N-PhiM

Use

Cre/Lox

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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Triple ORF FRT ifgMosaic Donor (IG135)

Plasmid

#99626

Purpose

To clone the 3 ORFs for FlpO dependent generation of ifgMosaics

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

PGK-Neo

Use

Cre/Lox, Mouse Targeting, and Synthetic Biology

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pJTB115

Plasmid

#248909

Purpose

Expresses yeast Smk1 and Ssp2 fused to glutathione-S-transferase in bacteria

Depositor

Edward Winter

Article

Tio et al Mol Cell Biol. 2017 May 2;37(10):e00607-

Inserts

SMK1 Mitogen-activated Protein Kinase (SMK1 Budding Yeast, Sequence is from the SK1 isolate of S. cerevisiae and will differ by 1 non-synonomous/several synonymous changes)
Ssp2 (SSP2 Budding Yeast, Sequence is from the SK1 isolate of S. cerevisiae and may differ by synonymous changes from S288C form)

Tags

glutathione-S-transferase

Expression

Bacterial

Mutation

NdeI and NcoI sites were removed for cloning purp…

Promoter

T7

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FRET_CST-C

Plasmid

#228572

Purpose

Expressed a FRET sensor for determining proteolytic cleavage of the C-terminal side of Catestatin

Depositor

Geert van den Bogaart

Article

Ioannidis et al J Endocr Soc. 2025 May 15;9(7):bvaf090.

Insert

Chromogranin-A (CHGA Human)

Tags

mCitrine and mScarlet-I

Expression

Mammalian

Mutation

FRET sensor; see explanatory note

Promoter

cmv

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FRET_CST-N

Plasmid

#228573

Purpose

Expressed a FRET sensor for determining proteolytic cleavage of the N-terminal side of Catestatin

Depositor

Geert van den Bogaart

Article

Ioannidis et al J Endocr Soc. 2025 May 15;9(7):bvaf090.

Insert

Chromogranin-A (CHGA Human)

Tags

mCitrine and mScarlet-I

Expression

Mammalian

Mutation

FRET sensor; see explanatory note

Promoter

cmv

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pMAL c2x Tral FL-15D

Plasmid

#113010

Purpose

For bacterial expression of MBP fusion of full-length Drosophila Tral with Phos-mimic mutations

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

full length tral (tral Fly)

Expression

Bacterial

Mutation

15 PNG phosphorylation sites mutated to D

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAc-GFP-dSUMOdeltaC

Plasmid

#85697

Purpose

expression of GFP-SUMO1 delta C in Drosophila cells

Depositor

Licio Collavin, Giannino Del Sal

Article

Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

Insert

SUMO (Sumo Fly)

Tags

GFP

Expression

Insect

Mutation

non-conjugatable SUMO, lacking the C-terminal gly…

Promoter

pAc5

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only
pMXS-IRES-Blast ACSL4

Plasmid

#173164

Purpose

Retroviral vector to express sgRNA resistant human ACSL4

Depositor

Kivanc Birsoy

Article

Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

Insert

Acyl-CoA Synthetase Long Chain Family Member 4 (ACSL4 Human)

Use

Retroviral

Mutation

Synonymous mutations at sgRNA sites

Promoter

MMLV

Available Since

Aug. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pX458M-53BP1-DN1S

Plasmid

#131045

Purpose

Plasmid encoding Cas9-53BP1-DN1S fusion with BbsI restriction sites for insertion of guide RNA sequence.

Depositor

Punam Malik

Article

Jayavaradhan et al Nat Commun. 2019 Jun 28;10(1):2866. doi:

Insert

SpCas9-53BP1-DN1S (TP53BP1 Human)

Use

CRISPR

Tags

GFP and SpCas9

Expression

Mammalian

Mutation

Only expressing residues 1231-1644 of complete 53…

Promoter

CBH

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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