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pRS426-HsFECH

Plasmid

#174524

Purpose

Expresses human ferrochelatase (FECH) in yeast

Depositor

Oleh Khalimonchuk

Article

Dietz et al Redox Biol. 2021 Oct;46:102125. doi: 10.

Insert

FECH with yeast HEM15 promoter and terminator (FECH Synthetic, Budding Yeast, Human)

Expression

Yeast

Mutation

Lacks original mitochondrial targeting sequence (…

Promoter

HEM15 (yeast, 257 bp)

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pRCC-N

Plasmid

#81192

Purpose

Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

Depositor

Eckhard Boles

Article

Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

Inserts

Cas9
empty gRNA cassette

Use

CRISPR

Tags

NTS

Expression

Yeast

Mutation

Please see depositor comments below. and WT - cod…

Promoter

ROX3 and SNP52p

Available Since

Sept. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pRCC-K

Plasmid

#81191

Purpose

Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance

Depositor

Eckhard Boles

Article

Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

Inserts

Cas9
empty gRNA cassette

Use

CRISPR

Tags

NTS

Expression

Yeast

Mutation

Please see depositor comments below. and WT - cod…

Promoter

ROX3 and SNP52p

Available Since

Sept. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pBF3060

Plasmid

#26853

Purpose

Expression of yeast codon-optimized CreA recombinase from the GAL1 promoter

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Insert

CreA recombinase

Use

Cre/Lox

Expression

Yeast

Mutation

N/A

Promoter

GAL1

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pRS423-GPD-wrmScarlet1-10-CYC1 terminator

Plasmid

#158584

Purpose

Expresses wrmScarlet1-10 in S. cerevisiae

Depositor

Cynthia Kenyon

Article

Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

Insert

wrmScarlet1-10

Expression

Yeast

Promoter

GPD

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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RCP83

Plasmid

#163466

Purpose

Backbone plasmid for insertion of the Saccharomyces cerevisiae promoter library and yeGFP

Depositor

Frank Albert

Article

Renganaath et al Elife. 2020 Nov 12;9. pii: 62669. doi: 1

Inserts

Promoter fragment (Sharon2012_1922)
His3 minimal promoter
yeGFP

Expression

Bacterial and Yeast

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pRG705_lexO-AsiSI_LexA-TF_LEU2MX

Plasmid

#154817

Purpose

Inducible expression of AsiSI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

AsiSI endonuclease
LexA-ER-B112

Tags

FLAG and NLS

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pRG706_lexO-SbfI_LexA-TF_LEU2MX

Plasmid

#154818

Purpose

Inducible expression of SbfI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

SbfI endonuclease
LexA-ER-B112

Tags

FLAG and NLS

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pRG659_lexO-FseI_LexA-TF_LEU2MX

Plasmid

#154816

Purpose

Inducible expression of FseI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

FseI endonuclease
LexA-ER-B112

Tags

FLAG and NLS

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pRG645_lexO-AscI_LexA-TF_LEU2MX

Plasmid

#154813

Purpose

Inducible expression of AscI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

AscI endonuclease
LexA-ER-B112

Tags

FLAG and NLS

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pRG714_lexO-AscI_LexA-TF_URA3MX

Plasmid

#154822

Purpose

Inducible expression of AscI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

AscI endonuclease
LexA-ER-B112

Tags

FLAG and NLS

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pET151-Vps4

Plasmid

#87733

Purpose

Bacterial expression of full-length S. cerevisiae Vps4

Depositor

Chris Hill

Article

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)

Tags

6xHis-V5-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-ScVrg4

Plasmid

#102334

Purpose

Expresses WT ScVrg4 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Parker et al Nature. 2017 Nov 23;551(7681):521-524. d

Insert

YGL225W (VRG4 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

GAL1

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pHES609 [prNOp7-PBAC]

Plasmid

#103017

Purpose

A S. cerevisiae plasmid. A photosensitive adenylate cyclase that produces cAMP in response to blue light. Used to activate PKA.

Depositor

Hana El-Samad

Article

Stewart-Ornstein et al Mol Biol Cell. 2017 Jan 1;28(1):221-227.

Insert

bPAC

Expression

Yeast

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pILGFPQ4

Plasmid

#83559

Purpose

Used to evaluate the expression output of GAL7 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

Insert

GAL7 promoter-yEGFP

Expression

Yeast

Promoter

GAL7

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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Dom34(full length)

Plasmid

#19098

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Protein DOM34 (DOM34 Budding Yeast)

Tags

His

Expression

Bacterial

Available Since

June 22, 2010

Availability

Academic Institutions and Nonprofits only
pYES2.1-cerMET5

Plasmid

#107437

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

MET5 (MET5 Budding Yeast)

Tags

V5-His

Expression

Yeast

Mutation

V81I, K341E, P597L, Y605C, E741G, D932G, N1217S, …

Promoter

GAL1

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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JBEI-6833

Plasmid

#84983

Purpose

Expression of NudB (E. coli) and PMD (S. cerevisiae) are under control of Trc promoter for isopentenol production.

Depositor

Taek Soon Lee

Article

George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

Insert

NudB-PMD

Expression

Bacterial

Promoter

trc promoter

Available Since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pRG711_lexO-AscI_LexA-TF_HIS3MX

Plasmid

#154820

Purpose

Inducible expression of AscI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

AscI endonuclease
LexA-ER-B112

Tags

FLAG and NLS

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pET151-Vta1(280-330)

Plasmid

#87738

Purpose

Bacterial expression of the VSL domain of S. cerevisiae Vta1

Depositor

Chris Hill

Article

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein Vta1 (VTA1 Budding Yeast)

Tags

6xHis-V5-TEV

Expression

Bacterial

Mutation

deleted amino acids 1-279

Promoter

T7

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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