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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,551 results for: Pet2

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Showing: 2901 - 2920 of 6551 results

  1. Traptavidin-E6

    Plasmid
    #59549
    Purpose
    pET21-Tre for expression of traptavidin (high stability mutant of streptavidin) bearing a glutamic acid tag for ion-exchange chromatography
    Depositor
    Mark Howarth
    Article
    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-
    Insert
    Core Traptavidin-E6
    Tags
    E6 tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  2. 9AVA

    Plasmid
    #220884
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TREX1 (TREX1 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 20, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    220884-plasmid-map-sequence-id-436793

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  3. KE-005

    Plasmid
    #133440
    Purpose
    Human elF4A1 residues 1-406, N-term His tag, thrombin cleavage, in pET21 vector backbone.
    Depositor
    Gerhard Wagner
    Insert
    EIF4A1 (EIF4A1 Human)
    Expression
    Bacterial
    Available Since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133440-plasmid-map-sequence-id-267708

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  4. RG-001

    Plasmid
    #133439
    Purpose
    Heat 3 domain of human elF4G in pET21 backbone.
    Depositor
    Gerhard Wagner
    Insert
    EIF4G1 (EIF4G1 Human)
    Expression
    Bacterial
    Available Since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    133439-plasmid-map-sequence-id-269573

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  6. Dead Streptavidin-SpyTag

    Plasmid
    #59548
    Purpose
    pET21-DTag encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin tetramers
    Depositor
    Mark Howarth
    Article
    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-
    Insert
    Dead Streptavidin-SpyTag
    Tags
    SpyTag
    Expression
    Bacterial
    Mutation
    The construct lacks final K of SpyTag
    Promoter
    T7
    Available Since
    Oct. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  8. KE-009

    Plasmid
    #133441
    Purpose
    Human elF4H, residue 1-228 (full-length), N-terminal His, GB1, TEV; in pET21 backbone.
    Depositor
    Gerhard Wagner
    Insert
    EIF4H (EIF4H Human)
    Expression
    Bacterial
    Available Since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133441-plasmid-map-sequence-id-267720

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  9. 8VL7

    Plasmid
    #220883
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TREX1 (TREX1 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 20, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    220883-plasmid-map-sequence-id-436777

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  12. Dead Streptavidin-SpyCatcher

    Plasmid
    #59547
    Purpose
    pET21-DCatch encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyCatcher, for generation of chimeric SpyAvidin tetramers
    Depositor
    Mark Howarth
    Article
    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-
    Insert
    Dead-dloop-Streptavidin-SpyCatcher
    Tags
    N-terminally shortened SpyCatcher
    Expression
    Bacterial
    Mutation
    PolyAsp in L3/4 loop for ion-exchange
    Available Since
    Oct. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. pEcRNAP5

    Plasmid
    #128939
    Purpose
    pET21a derivative encoding E. coli core RNA polymerase subunits: RpoAppx234-241-(His)10, B, C, Z
    Depositor
    Seth Darst
    Article
    Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:
    Inserts
    RpoAppx234-241-(His)10 (rpoA Escherichia coli)
    RpoB
    RpoC
    RpoZ
    Tags
    His
    Expression
    Bacterial
    Mutation
    Residues 234-241 are substituted for a PreScissio…
    Promoter
    T7
    Available Since
    Aug. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    128939-plasmid-map-sequence-id-254599

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Showing: 2901 - 2920 of 6551 results