We narrowed to 5,085 results for: mcs

1. pCDH-CMV-Nluc-P2A-copGFP-T2A-Puro

   Plasmid

   #73037

   Purpose

   Express Nluc and copGFP dual reporter

   Depositor

   Kazuhiro Oka

   Insert

   Nluc-P2A-copGFP-T2A-Puro
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-TRE-TdTomato

   Plasmid

   #110338

   Purpose

   TdTomato reporter vector of iTango and Cal-Light system for AAV production

   Depositor

   Hyungbae Kwon

   Insert

   tetO-TdTomato
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   tetO

   Available Since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDH-EF1S-Nluc-P2A-copGFP-T2A-Puro

   Plasmid

   #73032

   Purpose

   Express fluorescent and luminescence dual reporter

   Depositor

   Kazuhiro Oka

   Insert

   Nluc-P2A-copGFP-T2A-Puro
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Promoter

   Truncated EF1

   Available Since

   Jan. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. AICSDP-49: MYL2-mEGFP

   Plasmid

   #114414

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human MYL2, via Cas9-excisable CAGGS-mCherry selection cassette

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

   Insert

   MYL2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (MYL2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKL038 Lenti U6 dCas12a gRNA Puro mCherry

   Plasmid

   #195546

   Purpose

   dCas12a gRNA expression backbone

   Depositor

   Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Lenti U6- empty cassette_Direct repeat_puro_mcherry
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA5/FRT-Lyn11-mEGFP-cpHalo∆-(GGS)9-FKBP-P2A-Hpep3-(GGS)3-FRB-mScarlett

   Plasmid

   #205703

   Purpose

   CMV driven co-expression of split HaloTag FKBP- and FRB-fusions (Lyn11-EGFP-FKBP-cpHalo∆ and Hpep1-FRB-mScarlett) in mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Lyn11-EGFP-FKBP-cpHalo∆-P2A-Hpep1-FRB-mScarlett
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-FRT-stop-FRT(FSF)- loxP-stop-loxP(LSL)-SP-HA-HRPtm-WPRE-bGHpolyA

   Plasmid

   #197044

   Purpose

   In situ cell-surface proteome extraction by extracellular labeling (iPEEL)

   Depositor

   Liqun Luo

   Article

   Shuster et al Neuron. 2022 Dec 7;110(23):3882-3896.e9.

   Inserts

   pCAG-FSF-LSL
   SP-HA-HRPtm
   WPRE-bGHpolyA
   

   Tags

   HA, myc

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. c-myc-PT3EF1a

   Plasmid

   #92046

   Purpose

   Expresses c-Myc in mammalian cells

   Depositor

   Xin Chen

   Article

   Liu et al Hepatology. 2017 Mar 30. doi: 10.1002/he

   Insert

   C-MYC (MYC Human)
   

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   July 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX304-KDM6A

   Plasmid

   #203580

   Purpose

   Expresses wild-type KDM6A in mammalian cells.

   Depositor

   William Kaelin, Abhishek Chakraborty

   Article

   Chakraborty et al Science. 2019 Mar 15;363(6432):1217-1222

   Insert

   KDM6A (KDM6A Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-CBA-dt/PGK-CFTR-⌀

    Plasmid

    #163923

    Purpose

    Expresses human CFTR.

    Depositor

    John Engelhardt

    Article

    Choi et al Genes (Basel). 2020 Oct 6;11(10). pii: g

    Insert

    CFTR (CFTR Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIG-601_NY-ESO-1_TCR_GFP

    Plasmid

    #207482

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    GFP, NY-ESO-1_TCR
    

    Use

    CRISPR

    Mutation

    Potential silent point mutation in GFP sequence

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-M-Flag-BAP1

    Plasmid

    #125840

    Purpose

    express wild type BAP1

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Insert

    BAP1 (BRCA1 associated protein 1) (BAP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-M-Flag-BAP1-C91A

    Plasmid

    #125841

    Purpose

    express mutant BAP1-C91A

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Insert

    BAP1 (BRCA1 associated protein 1) (BAP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Mutation

    C91A

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. FLAG-MARK3-CR

    Plasmid

    #229422

    Purpose

    Lentiviral or overpexression of cDNA

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    MARK3-CR (MARK3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    silent mutation CRISPR resistant

    Promoter

    EFS

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX-M-Flag-BAP1-C91G

    Plasmid

    #125845

    Purpose

    express mutant BAP1-C91G

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Insert

    BAP1 (BRCA1 associated protein 1) (BAP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Mutation

    C91G

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIG-602_NY-ESO-1_TCR_RFP

    Plasmid

    #207483

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    RFP, NY-ESO-1_TCR
    

    Use

    CRISPR

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIG-831_HA-GD2-28z_CAR_TFAP4_Retroviral

    Plasmid

    #207508

    Purpose

    This plasmid can be used to generate retrovirus.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    TFAP4, HA-GD2-28z_CAR (TFAP4 Human)
    

    Use

    Retroviral

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTwist-SARS-CoV-2 Spike-deltaC18 JN.1

    Plasmid

    #216526

    Purpose

    Encodes SARS-CoV-2 spike for JN.1 pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike JN.1 (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    See Depositor Comments.

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGIPz-PD-L1 WT

    Plasmid

    #121486

    Purpose

    2nd generation lentiviral vector for over expressing human PD-L1 WT with PD-L1 shRNA

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    PD-L1
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. C1ORF43(1-41)-3xFLAG

    Plasmid

    #130881

    Purpose

    Expresses first 41 amino acids of human C1ORF43 with C-terminal 3xFLAG tag

    Depositor

    Michael Bassik

    Article

    Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

    Insert

    C1ORF43 (C1orf43 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Deleted amino acids 44-253

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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