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pHA850 - osm-10p::FynY531F

Plasmid

#139207

Purpose

Express activated Fyn kinase in osm-10 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

Fyn (FYN Human)

Expression

Worm

Mutation

Y531F

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA851 - osm-10p::Fynempty

Plasmid

#139208

Purpose

Control vector for Fyn kinase expression in osm-10 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

Fyn (FYN Human)

Expression

Worm

Mutation

deletion and frame shift to prevent Fyn expression

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pWPXL-p150-PIP1Δ

Plasmid

#160993

Purpose

Express mouse p150 protein with PIP1 deletion mutation

Depositor

Zhiguo Zhang

Article

Cheng et al Nucleic Acids Res. 2019 Dec 2;47(21):111

Insert

CHAF1A (Chaf1a Mouse)

Tags

GFP

Expression

Mammalian

Mutation

PIP1 (PCNA-interacting protein) motif deletion

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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Oct4deltaPE-53BP1mCherry

Plasmid

#141122

Purpose

Construct used to generate a transgenic mouse strain with expression of 53BP1-mCherry in embryonic germ cells

Depositor

John Schimenti

Article

Bloom et al Genesis. 2020 Aug;58(8):e23368. doi: 10.

Insert

Fragment of human 53BP1 (TP53BP1 Human)

Use

Mouse Targeting

Tags

mCherry

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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AdMKP-3 S159A

Plasmid

#131520

Purpose

Expresses MKP-3 with S159A mutation in mammalian cells

Depositor

Haiyan Xu

Article

Feng et al Mol Cell Endocrinol. 2012 Sep 25;361(1-2

Insert

Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)

Use

Adenoviral

Tags

No

Mutation

changed Serine 159 to Alanine

Promoter

CMV

Available Since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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gh111

Plasmid

#106778

Purpose

expression of gRNA targeting B3GALNT1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

B3GALNT1 (B3GALNT1 Human)

Expression

Mammalian

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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rat brain inositol 1,4,5-trisphosphate 3-kinase A

Plasmid

#122155

Purpose

rat brain cDNA encoding a Ca2+/calmodulin-sensitive inositol 1,4,5-trisphosphate 3-kinase A; clone CP16 (full length)

Depositor

Christophe Erneux

Article

Takazawa et al Biochem J. 1990 Nov 15;272(1):107-12.

Insert

inositol 1,4,5-trisphosphate 3-kinase A (Itpka Rat)

Expression

Bacterial

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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gh115

Plasmid

#106785

Purpose

expression of gRNA targeting GCNT1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GCNT1 (GCNT1 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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gh118

Plasmid

#106788

Purpose

expression of gRNA targeting GCNT4

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GCNT4 (GCNT4 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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gh72

Plasmid

#106732

Purpose

expression of gRNA targeting B3GNT6

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

B3GNT6 (B3GNT6 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac-GST-Mps1-KD

Plasmid

#114066

Purpose

protein production

Depositor

Geert Kops

Article

Jelluma et al PLoS One. 2008 Jun 11;3(6):e2415. doi: 1

Insert

Mps1-KD (TTK Human)

Tags

GST

Expression

Insect

Mutation

Mps1 KD (D664A)

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac-GST-Mps1-WT

Plasmid

#114065

Purpose

protein production

Depositor

Geert Kops

Article

Jelluma et al PLoS One. 2008 Jun 11;3(6):e2415. doi: 1

Insert

Mps1-WT (TTK Human)

Tags

GST

Expression

Insect

Mutation

none

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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APBA1_PDZ_1

Plasmid

#103942

Purpose

Protein expression and purification of APBA1 PDZ1 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

APBA1 (APBA1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARHGEF19-1/1

Plasmid

#91432

Purpose

Protein expression and purification of human SH3 domain construct ARHGEF19-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGEF19-1/1 (ARHGEF19 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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CSII-IDR2-owlTRIMCyp-FOS K283D Q287D

Plasmid

#79068

Purpose

Express owl monkey TRIMCyp K283D, Q287D in mammalan cells

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5Cyp (owl monkey) (K283D, Q287D)-FOS

Tags

OneSTrEP-FLAG

Expression

Mammalian

Mutation

Changed Lys 283 to Asp and Gln 287 to Asp

Promoter

CMV

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX4T-Mst2 SARAH

Plasmid

#84298

Purpose

Expresses Mst2 SARAH domain for interaction and spectroscopy studies.

Depositor

Jonathan Chernoff

Article

Rawat et al Curr Biol. 2016 Jun 20;26(12):1556-63. d

Insert

Mst2 (STK3 Human)

Tags

GST

Expression

Bacterial

Mutation

L443W

Promoter

tac

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-FLAG-WRAP53beta-siRNA-resistant

Plasmid

#64680

Purpose

expresses siRNA resistant WRAP53beta

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Tags

FLAG

Expression

Mammalian

Mutation

siRNA resistant

Promoter

CMV

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)Q30D

Plasmid

#60346

Purpose

Q30D mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

Q30D; contains amino acids 7-192 of Msi1

Promoter

T7

Available Since

Dec. 1, 2014

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)R53E

Plasmid

#60352

Purpose

R53E mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

R53E; contains amino acids 7-192 of Msi1

Promoter

T7

Available Since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)G64A

Plasmid

#60351

Purpose

G64A mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

G64A; contains amino acids 7-192 of Msi1

Promoter

T7

Available Since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only