We narrowed to 13,032 results for: cdna

1. pcDNA3.1_mGFP-FGFR3-G380R

   Plasmid

   #191755

   Purpose

   Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-G380R mutation (associated with ACH; target mutation: c.1138G>A; silent mutation added on purpose: c.1131C>T)

   Depositor

   Irene Tiemann-Boege

   Article

   Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

   Insert

   mGFP-FGFR3IIIc
   

   Use

   Tags

   mGFP

   Expression

   Mammalian

   Mutation

   c.1138G>A (G380R) + c.1131C>T (silent mutat…

   Promoter

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 FLIPE-600n

   Plasmid

   #13541

   Depositor

   Wolf Frommer

   Article

   Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

   Insert

   FLIP-E WT
   

   Use

   Tags

   ECFP and Venus

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)_Paragranulin-no linker

   Plasmid

   #176917

   Purpose

   express Paragranulin without linker 1 in mammalian cells

   Depositor

   Thomas Kukar

   Article

   Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

   Insert

   Paragranulin (GRN Human)
   

   Use

   Tags

   Twin-Strep and FLAG tags encoded after Signal Pep…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)_Paragranulin+linker 1

   Plasmid

   #176915

   Purpose

   express Paragranulin with linker 1 in mammalian cells

   Depositor

   Thomas Kukar

   Article

   Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

   Insert

   Paragranulin + linker 1 (GRN Human)
   

   Use

   Tags

   Twin-Strep and FLAG tags encoded after Signal Pep…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.3-hALDOA D34S

   Plasmid

   #210810

   Purpose

   To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)

   Depositor

   Sheng-cai Lin

   Article

   Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

   Insert

   ALDOA (ALDOA Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   residues 34 of ALDOA mutated to serine

   Promoter

   CMV

   Available since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-gLuc-C1C2

   Plasmid

   #178426

   Purpose

   Bioluminescence reporter fused to EV membrane binding domain for EV imaging and tracking

   Depositor

   Masako Harada

   Article

   Komuro et al Nanotheranostics. 2021 Apr 15;5(4):378-3

   Insert

   gLuc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1: TfR1-CoilE

   Plasmid

   #181988

   Purpose

   CoilE genetically encoded tag on Transferrin Receptor 1 (TfR1) to be used with CoilR labeled with a reporter

   Depositor

   Kimberly Beatty

   Article

   Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

   Insert

   TfR1-CoilE
   

   Use

   Tags

   CoilE

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-6His-MyoX

   Plasmid

   #47607

   Depositor

   Emanuel Strehler

   Article

   Bennett et al Biochem Biophys Res Commun. 2008 May 2;3

   Insert

   MyoX (MYO10 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA5FRT/TO-DVC1-Strep-HA

   Plasmid

   #113481

   Purpose

   Expression of human DVC1 with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   DVC1 (SPRTN Human)
   

   Use

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3-Flag-hDcp2

    Plasmid

    #72213

    Purpose

    mammalian expression of Flag-tagged human Dcp2

    Depositor

    Megerditch Kiledjian

    Article

    Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

    Insert

    Dcp2 (DCP2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Q170R (please see depositor's comment below)

    Promoter

    CMV

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-FLAG-MKRN1_H307E

    Plasmid

    #78756

    Purpose

    To overexpress MKRN1_H307E in Mammalian Cell

    Depositor

    Jaewhan Song

    Article

    Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

    Insert

    MKRN1 (MKRN1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    H307E

    Promoter

    CMV

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3 MKK7b1(K149A)

    Plasmid

    #14674

    Depositor

    Roger Davis

    Article

    Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

    Insert

    MKK7b1(K149A) (Map2k7 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Lys-149Ala

    Promoter

    Available since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA RBD-GFP11

    Plasmid

    #133069

    Purpose

    Expresses RBD domain with tripartite split GFP11 (M4)

    Depositor

    Stéphanie Cabantous

    Article

    Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

    Insert

    RBD (Rhotekin-Rho Binding Domain)
    

    Use

    Tags

    GFP11 (tripartite split-GFP)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1 FLIPglu-170nDelta13V

    Plasmid

    #18018

    Depositor

    Wolf Frommer

    Article

    Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

    Insert

    FLIPglu F16F Delta13
    

    Use

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    WT binding protein (no F16A)

    Promoter

    Available since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1+ Flag-RAGB99L

    Plasmid

    #128101

    Purpose

    Expresses constitutively GTP-bound RAGB in mammalian cells in a G418-selectable plasmid

    Depositor

    Chi Van Dang

    Article

    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

    Insert

    Ras related GTP binding B (RRAGB Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Q99L (CAA to CTA)

    Promoter

    CMV

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-JNKAR1-NES

    Plasmid

    #84632

    Purpose

    Unimolecular, FRET-based JNK activity reporter; cytosol targeted

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    ECFP-FHA1-JNKsubDD-Citrine-NES
    

    Use

    Tags

    6xHis, Citrine, ECFP, Nuclear export signal (NES)…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-HA-RIPK1 K115R

    Plasmid

    #78835

    Purpose

    To overexpress RIPK1 K115R in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K115R

    Promoter

    CMV

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-HA-ERK2 Y261A

    Plasmid

    #8977

    Depositor

    John Blenis

    Article

    Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

    Insert

    ERK2 (Mapk1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Y261A

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

19. pCDNA HA FAK Y861F

    Plasmid

    #50508

    Depositor

    Kenneth Yamada

    Article

    Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

    Insert

    protein tyrosine kinase, mutated
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Y861F

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3-FLAG_FBXL7 (R310H)

    Plasmid

    #171849

    Purpose

    pcDNA3-FLAG_FBXL7 (R310H)

    Depositor

    Michele Pagano

    Article

    Moro et al Nat Cell Biol. 2020 Sep;22(9):1130-1142.

    Insert

    F-box and leucine rich repeat protein 7 (FBXL7 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed Arginine 310 to Histidine

    Promoter

    Available since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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