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pcDNA3.1-dCas9-CibN

Plasmid

#60552

Purpose

Expresses dCas9-CibN in mammalian cells

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

dCas9-CibN

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

inactivating Cas9 mutations D10A and H840A

Promoter

CMV

Available Since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pETconNK-TEM1(S70A,D179G)

Plasmid

#81166

Purpose

pETconNK with TEM1(S70A,D179G) beta-lactamase

Depositor

Timothy Whitehead

Article

Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

Insert

TEM-1 beta-lactamase

Tags

c-myc

Expression

Bacterial and Yeast

Mutation

Deleted the sec transport tag. Plasmid encodes H2…

Promoter

GAL1 and gal1

Available Since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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mRIP3 GFP D161N

Plasmid

#41383

Depositor

Francis Chan

Article

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP3 (Ripk3 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Kinase Domain Mutant (D161N)

Promoter

CMV

Available Since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only
Clover-PDHA1-N-10

Plasmid

#56304

Purpose

Localization: Mitochondria, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

PDHA1 (PDHA1 Human)

Tags

Clover

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits
p168

Plasmid

#62618

Purpose

ePathBrick pETM6 vector encoding CsF3H, FaDFR, and DuLAR

Depositor

Mattheos Koffas

Article

Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

Inserts

flavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase

Use

Synthetic Biology

Tags

GBD, PDZ, and SH3

Expression

Bacterial

Promoter

T7

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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Lyn-CFP-GAI(1-92)

Plasmid

#37311

Depositor

Takanari Inoue

Article

Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

Insert

GAI (GAI Mustard Weed)

Tags

CFP and Lyn

Expression

Mammalian

Mutation

contains aa 1-92

Promoter

CMV

Available Since

June 15, 2012

Availability

Academic Institutions and Nonprofits only
JW617

Plasmid

#64809

Purpose

CUC3 expression in yeast

Depositor

Detlef Weigel

Article

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

CUC3 (CUC3 Mustard Weed)

Tags

GAL4 DNA binding domain and Myc

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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JW624

Plasmid

#64808

Purpose

CUC2 expression in yeast

Depositor

Detlef Weigel

Article

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

CUC2 (CUC2 Mustard Weed)

Tags

GAL4 DNA binding domain

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR MCS L3-L2

Plasmid

#62088

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-lxlplap

Plasmid

#45581

Depositor

Nirao Shah

Article

Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

Inserts

Green fluorescent protein
Placental alkaline phosphatase (ALPP Human)

Use

Lentiviral

Tags

Histone 2B

Expression

Mammalian

Promoter

Ubiquitin

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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mRuby2-Nup50-N-10

Plasmid

#55908

Purpose

Localization: Nuclear Pore, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Nup50 (NUP50 Human)

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pME-mKate-P2A-APEX2-NS

Plasmid

#67670

Purpose

Gateway entry clone containing mKate2-P2A-APEX2 with no stop codon

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-NS

Use

Gateway multisite middle entry clone

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET28-At_PRORP2-His

Plasmid

#67870

Purpose

bacterial expression of PRORP2 (At2g16650), full coding sequence + C-term. His tag

Depositor

Walter Rossmanith

Article

Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

Insert

PRORP2 (PRORP2 Mustard Weed)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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PhyB-mCherry-NLS

Plasmid

#51571

Purpose

Constitutively express nuclear localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Article

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Tags

NLS and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available Since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pAL190

Plasmid

#22279

Purpose

Expresses Tim DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

TimDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)

Use

Synthetic Biology

Expression

Mammalian

Promoter

SV40

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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PhyB-mCherry-Htb2

Plasmid

#51569

Purpose

Constitutively express nuclear localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Article

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Tags

HTB2 and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available Since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR MCS L5-L2

Plasmid

#62085

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV L1-R5

Plasmid

#62090

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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NLS-GAI(1-92)

Plasmid

#37317

Depositor

Takanari Inoue

Article

Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

Insert

GAI (GAI Mustard Weed)

Tags

NLS

Expression

Mammalian

Mutation

contains aa 1-92

Promoter

CMV

Available Since

June 15, 2012

Availability

Academic Institutions and Nonprofits only
LentiCRISPR-sgUFSP2

Plasmid

#86134

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting UFSP2

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

sgRNA targeting UFSP2 (UFSP2 )

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

May 15, 2017

Availability

Academic Institutions and Nonprofits only

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