We narrowed to 3,960 results for: LYC

1. SARS_COV2_M_HA_BASU

   Plasmid

   #153990

   Purpose

   Express M gene along with HA and BASU

   Depositor

   Paul Khavari

   Article

   Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

   Insert

   SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. PET-B-HypaSpCas9-NLS-6xHis

   Plasmid

   #207386

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HypaSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N692A, M694A, Q695A, H698A, amino acids 1005-1013…

   Promoter

   T7

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmirGLO-Bmpr2-1719

   Plasmid

   #49379

   Purpose

   Insert is a fragment of the Bmpr2 3'-UTR that contains miR-17 and miR-19 binding sites. To be used for 3'UTR assay.

   Depositor

   Martine Roussel

   Article

   Murphy et al Cancer Res. 2013 Oct 21.

   Insert

   Bmpr2 (BMPR2 Human, Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Human phosphoglycerate kinase (PGK) promoter

   Available since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRP(Exp)-CMV> ORF_924bp (Azgp1):T2A:dTomato:IRES:Puro

   Plasmid

   #110830

   Purpose

   Expresses zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter where both sequences are separated by a T2A self-cleaving peptide sequence as a result, the dT is not fused with the ZAG protein.

   Depositor

   Ande Satyanarayana

   Article

   Elattar et al FASEB J. 2018 Mar 23:fj201701465RR. doi:

   Insert

   AZGP1 (Azgp1 Mouse)
   

   Use

   Non-viral gene expression vector

   Tags

   T2A and dTomato

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCI-neo-myc-LC3(G120A)-HA

   Plasmid

   #45245

   Depositor

   Tamotsu Yoshimori

   Article

   Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Tags

   HA and myc

   Expression

   Mammalian

   Mutation

   Glycine at position 120 mutated to Alanine

   Promoter

   Available since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pJS699

   Plasmid

   #168779

   Purpose

   Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) and can be combined with pJS697 through homologous recombination

   Depositor

   Timothy Whitehead

   Article

   Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

   Insert

   Spike RBD (S betacoronavirus)
   

   Use

   Tags

   Cmyc

   Expression

   Mutation

   N343Q

   Promoter

   GAL1

   Available since

   Aug. 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21c-His-Xa-Gluc-Xa-C9D-TGinsert

   Plasmid

   #124660

   Purpose

   reporter protein which emits bright blue fluorescence

   Depositor

   Yutaka Kuroda

   Article

   Wu et al Sci Rep. 2020 Nov 18;10(1):20069. doi: 1

   Insert

   Gaussia luciferase-C9D-TGinsert
   

   Use

   Tags

   IEGRTMGDDDGDDDGDDD（FactorXa,TMG,C9D-Tag） and MHHH…

   Expression

   Bacterial

   Mutation

   changed glutamic acid 100 to alanine, and glycine…

   Promoter

   T7 promoter

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBabe puro-HA-PIK3CA(H1047R;G320E)

   Plasmid

   #33254

   Depositor

   Thomas Roberts

   Article

   Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

   Insert

   PIK3CA (PIK3CA Human)
   

   Use

   Retroviral

   Tags

   Hemagglutinin (HA)

   Expression

   Mammalian

   Mutation

   changed histidine 1047 to arginine and glycine 32…

   Promoter

   SV40

   Available since

   Jan. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

9. Pf113-bio

   Plasmid

   #47729

   Purpose

   Expresses enzymatically monobiotinylated full-length Pf113 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised Pf113
   

   Use

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV 3xFLAG-LC3A G120A

    Plasmid

    #123090

    Purpose

    Expresses 3xFLAG-LC3A G120A in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Glycine 120 to Alanine

    Promoter

    CMV

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET32a-Spike-403-505

    Plasmid

    #165095

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 spike protein site 403-505 (S SARS-CoV2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pCMV 3xFLAG-GABARAPL1 G116A

    Plasmid

    #123101

    Purpose

    Expresses 3xFLAG-GABARAPL1 G116A in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Glycine 116 to Alanine

    Promoter

    CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. WT N-His Ana GvpC in pET28a

    Plasmid

    #153294

    Purpose

    Expresses N-terminal His-tagged GvpC from Anabaena flos-aquae in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    Gas vesicle protein C (N-terminal his-tagged)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Added a hexahistidine tag after the methionine at…

    Promoter

    T7

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. GAMA-bio

    Plasmid

    #47747

    Purpose

    Expresses enzymatically monobiotinylated full-length GAMA ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised GAMA
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. His-DNPH1

    Plasmid

    #203224

    Purpose

    For protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

    Depositor

    Vern Schramm

    Article

    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

    Insert

    DNPH1 (DNPH1 Human)
    

    Use

    Tags

    His tag (with TEV cleavage site)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223 GABARAPL2 G116A

    Plasmid

    #123087

    Purpose

    Gateway entry clone encoding human GABARAPL2 G116A

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
    

    Use

    Gateway entry vector / entry clone

    Tags

    Expression

    Mutation

    Glycine 116 to Alanine

    Promoter

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. GB1-PRD(703-868, P801G)-Strep

    Plasmid

    #164444

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Elias et al J Biol Chem. 2021 Oct 21:101328. doi: 10

    Insert

    GB1-His6-TEV-ALIX(703-868, P801G)-Strep (PDCD6IP Human)
    

    Use

    Tags

    GB1-His6-TEV and Strep tag (WSHPQFEK)

    Expression

    Bacterial

    Mutation

    Proline 801 to Glycine

    Promoter

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHEN-CoV2-nanobody-Ty1

    Plasmid

    #194588

    Purpose

    Bacterial expression of Ty1 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

    Depositor

    Gerald McInerney

    Article

    Hanke et al Nat Commun. 2020 Sep 4;11(1):4420. doi:

    Insert

    Ty1 (S )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIRESneo-MPG(N169D)

    Plasmid

    #23259

    Depositor

    Robert Sobol

    Article

    Tang et al Mol Cancer Res. 2010 Jan 12. ():.

    Insert

    N-methylpurine DNA glycosylase (N169D) (MPG Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N169D

    Promoter

    Available since

    March 31, 2010

    Availability

    Academic Institutions and Nonprofits only

20. eGFP-KIF5A-Rigor

    Plasmid

    #172202

    Purpose

    Expresses KIF5A G235A rigor mutant tagged with eGFP in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Farias et al Cell Rep. 2015 Nov 10;13(6):1221-1232. d

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Changed Glycine 235 to Alanine

    Promoter

    CMV

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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