We narrowed to 23,609 results for: Sele
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Plasmid#34817DepositorInsertintegrin-linked kinase-associated serine/threonine phosphatase 2C (ILKAP Human)
TagsHis and TEVExpressionBacterialAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGG426_UV5
Plasmid#165608PurposeVector for expression of the SpCas9 VRKG variant with sgRNA in E. coli: lacUV5-VRKG(SpCas9, D1135V/S1136R/D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)DepositorInsertlacUV5 driving Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G) and hEGFP-sgRNA
UseCRISPR and Synthetic BiologyExpressionBacterialMutationD1135V, S1136R, D1332K and R1333G mutations in Sp…PromoterlacUV5 driving Cas9 VRKG and UV5 driving sgRNAAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1-6xHis-TEV-Nsp8
Plasmid#201022PurposeBacterial expression of codon optimized SARS-CoV-2 nsp8 tagged with an N-terminus His-tag followed by a TEV protease cleavage site.DepositorInsertnon-structural protein 8 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
Tags6xHis-TEVExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro-sgULK1-1
Plasmid#109004PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorAvailable SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-Centrobin
Plasmid#136878PurposeMammalian expression of the centrosomal protein Centrombin N-terminally fused to CLIP-tagDepositorInsertCLIP-Centrobin (CNTROB Synthetic, Human)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131-STU-As
Plasmid#138094PurposeGolden Gate entry vector for 1st crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-CP110
Plasmid#136851PurposeMammalian expression of the centrosomal protein Cep110 N-terminally fused to CLIP-tagDepositorInsertCLIP-CP110 (CCP110 Synthetic, Human)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
HOXB1 (human) HIS-tag pET
Plasmid#8520DepositorAvailable SinceJuly 6, 2006AvailabilityAcademic Institutions and Nonprofits only -
pQC hKGA.wt V5-His IRES G418
Plasmid#110390Purposeγ-Retroviral transfer vector for expressing glutminase isoform KGA (wild type), C-terminal V5-6xHis tags, IRES-driven Geneticin selection.DepositorInsertKGA glutaminase (GLS Human)
UseRetroviralTags6xHis and V5ExpressionMammalianPromoterCMVAvailable SinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-Cep164
Plasmid#136824PurposeMammalian expression of the centrosomal protein Cep164 N-terminally fused to SNAP-tagDepositorInsertSNAP-Cep164 (CEP164 Synthetic, Human)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-KIAA0841
Plasmid#136810PurposeMammalian expression of the centrosomal protein KIAA0841 N-terminally fused to SNAP-tagDepositorInsertSNAP-KIAA0841 (HAUS5 Synthetic, Human)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
HOXB3 (human) HIS-tag pET
Plasmid#8524DepositorInsertHOXB3 (HOXB3 Human)
TagsHis and T7ExpressionBacterialMutationContains 5 additional amino acids before ATG from…Available SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-A3-VN
Plasmid#135506PurposeMammalian expression of VN-fused and myc-tagged HLA-A*03:01DepositorInsertHLA-A*03:01 (HLA-A Human)
TagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianPromoterCMVAvailable SinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT131_GalL_cCDA1-NGBE3
Plasmid#145095PurposeExpressing base editor cCDA1-NGBE3 in yeast cellsDepositorInsertcCDA1-NGBE3
UseCRISPRExpressionYeastMutationspCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…PromoterGalLAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-161K enhancer-ETS-CORE-MUT-minP:fLuc-TK:rLuc
Plasmid#138367PurposeLentiviral plasmid contains 161k modified human enhancer with with ETS binding sites mutated driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoterDepositorInsert161K human enhancer, with ETS binding sites mutated, driving firefly enhancer and TK driving refills luciferase
UseLentiviralExpressionMammalianAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro-sgATL2-1
Plasmid#109008PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorAvailable SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag MKK3b(Glu, delete LXL)
Plasmid#50450Purposeactivates p38 in the signaling pathwayDepositorInsertMKK3b (MAP2K3 Human)
TagsFlagExpressionMammalianMutationdeleted L25-I27, changed Serine 218 to Glutamic A…PromoterCMVAvailable SinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-161K enhancer-NR2F2-MUT-minP:fLuc-TK:rLuc
Plasmid#138368PurposeLentiviral plasmid contains 161k modified human enhancer with with NR2F2 binding sites mutated driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoterDepositorInsert161K human enhancer, with NR2F2 binding sites mutated, driving firefly enhancer and TK driving renilla luciferase
UseLentiviralExpressionMammalianAvailable SinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ134-STU-Fn
Plasmid#138104PurposeGolden Gate entry vector for 4th crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131-STU-Fn
Plasmid#138095PurposeGolden Gate entry vector for 1st crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only