Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 18,824 results for: nls

Clear keywords
Showing: 2961 - 2980 of 18824 results

  1. enDR3-TEV-mEGFP-NLS (pRS1451)

    Plasmid
    #240546
    Purpose
    Expression of the engineered N-terminal hybrid-binding domain of RNase H3 (enDR3) for capturing R-loops. A TEV cleavage site, mEGFP and nuclear localization signal (NLS) are included in the fusion.
    Depositor
    Roman Szczęsny
    Publication
    Jedynak-Slyvka et al Nucleic Acids Res. 2025 Aug 11;53(15):gk
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    enDR3
    Expression
    Mammalian
    Mutation
    L52M
    Available since
    Aug. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    240546-plasmid-map-sequence-id-481872

    Enlarge

  2. nb-enDR3-TEV-mEGFP-NLS (pRS1458)

    Plasmid
    #240547
    Purpose
    Expression of negative control for R-loops capture experiments using enDR3. A mutated, non-R-loops binding version of enDR3 is expressed in fusion with the TEV cleavage site, mEGFP, and NLS.
    Depositor
    Roman Szczęsny
    Publication
    Jedynak-Slyvka et al Nucleic Acids Res. 2025 Aug 11;53(15):gk
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nb-enDR3
    Expression
    Mammalian
    Mutation
    S48A, K50A
    Available since
    Aug. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    240547-plasmid-map-sequence-id-481875

    Enlarge

  3. pCSD_2XNLS-SpCas9MT3-NLS-3XHA-2XNLS-NmdCas9-2XNLS

    Plasmid
    #107323
    Purpose
    Expresses SpCas9 (R1335K) fused to dNmCas9 in mammalian cells
    Depositor
    Scot Wolfe
    Publication
    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-NmCas9 fusion
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    SpCas9 (R1335K) is fused to nuclease dead NmCas9 …
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107323-plasmid-map-sequence-id-204717

    Enlarge

  4. pCAG-hAsCas12a-enRR(E174R/S542R/K607R)-NLS(nucleoplasmin)-3xHA (AAS1077)

    Plasmid
    #114095
    Purpose
    CAG promoter expression plasmid for human codon optimized AsCas12a-enRR(E174R/S542R/K607R) nuclease with C-terminal NLS and HA tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized AsCas12a-RR (E174R/S542R/K607R)
    Tags
    NLS(nucleoplasmin) and 3xHA
    Expression
    Mammalian
    Mutation
    E174R, S542R and K607R
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114095-plasmid-map-sequence-id-220556

    Enlarge

  14. pCS2-UTX-F-MT2

    Plasmid
    #40619
    Purpose
    Expression of enzyme-dead UTX in mammalian cells
    Depositor
    Kai Ge
    Publication
    Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    UTX (KDM6A Human)
    Tags
    6xMyc and Flag
    Expression
    Mammalian
    Mutation
    changed Histidine 1146 and Glutamic Acid 1148 to …
    Promoter
    CMV
    Available since
    Nov. 5, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
Showing: 2961 - 2980 of 18824 results