PurposeExpression of enzyme-dead UTX in mammalian cells
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||40619||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepCS2+ NLSMT
- Backbone size w/o insert (bp) 4400
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Alt nameubiquitously transcribed tetratricopeptide repeat, X chromosome
SpeciesH. sapiens (human)
Insert Size (bp)4200
Mutationchanged Histidine 1146 and Glutamic Acid 1148 to Alanine
Entrez GeneKDM6A (a.k.a. KABUK2, UTX, bA386N14.2)
- Promoter CMV
/ Fusion Proteins
- 6xMyc (N terminal on backbone)
- Flag (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site Xho I (destroyed during cloning)
- 3′ cloning site Xba I (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Full length human UTX with a C-terminal FLAG was PCR-amplified from MGC clone BC093868 and cloned into the XhoI-XbaI sites downstream of the CMV promoter and in frame with the N-terminal 6myc tag in the pCS2+ NLSMT vector. Two amino acids essential for UTX enzymatic activity, H1146 and E1148 are mutated to alanine.
UTX insert contains K220E, V503A, N606D, V607A, T726K, Y1173F point mutations relative to closest reference sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS2-UTX-F-MT2 was a gift from Kai Ge (Addgene plasmid # 40619 ; http://n2t.net/addgene:40619 ; RRID:Addgene_40619)
For your References section:UTX regulates mesoderm differentiation of embryonic stem cells independent of H3K27 demethylase activity. Wang C, Lee JE, Cho YW, Xiao Y, Jin Q, Liu C, Ge K. Proc Natl Acad Sci U S A. 2012 Sep 4. 10.1073/pnas.1204166109 PubMed 22949634
Map uploaded by the depositor.