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(Plasmid #40619)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 40619 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pCS2+ NLSMT
  • Backbone size w/o insert (bp) 4400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
    ubiquitously transcribed tetratricopeptide repeat, X chromosome
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    changed Histidine 1146 and Glutamic Acid 1148 to Alanine
  • GenBank ID
  • Entrez Gene
    KDM6A (a.k.a. KABUK2, UTX, bA386N14.2)
  • Promoter CMV
  • Tags / Fusion Proteins
    • 6xMyc (N terminal on backbone)
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho I (destroyed during cloning)
  • 3′ cloning site Xba I (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Full length human UTX with a C-terminal FLAG was PCR-amplified from MGC clone BC093868 and cloned into the XhoI-XbaI sites downstream of the CMV promoter and in frame with the N-terminal 6myc tag in the pCS2+ NLSMT vector. Two amino acids essential for UTX enzymatic activity, H1146 and E1148 are mutated to alanine.

UTX insert contains K220E, V503A, N606D, V607A, T726K, Y1173F point mutations relative to closest reference sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-UTX-F-MT2 was a gift from Kai Ge (Addgene plasmid # 40619 ; ; RRID:Addgene_40619)
  • For your References section:

    UTX regulates mesoderm differentiation of embryonic stem cells independent of H3K27 demethylase activity. Wang C, Lee JE, Cho YW, Xiao Y, Jin Q, Liu C, Ge K. Proc Natl Acad Sci U S A. 2012 Sep 4. 10.1073/pnas.1204166109 PubMed 22949634