-
Plasmid#204995PurposeExpresses shRNA resistant GFP-RepoMan S893D MutantDepositorInsertcell division cycle associated 2 (CDCA2 Human)
UseTagsGFPExpressionMammalianMutationchanged Serine 893 to Aspartic acidPromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28 mePOU-POU
Plasmid#206392PurposeExpresses the DNA binding domain of the ePOU with a 6xHis tag for protein purificationDepositorInsertePOU
UseTags6xHisExpressionBacterialMutationPOU DNA binding domain onlyPromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFsynW SYT1deltaC2AB reporter
Plasmid#197279PurposeEncodes for viral expression of Synaptotagmin 1 (SYT1) (1 - 140 a.a.) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).DepositorInsertSYT1 (1 - 420 a.a.) and HaloTag (Syt1 Rat)
UseLentiviralTagsFLAGExpressionMutationPromoterhSyn (human synapsin I promoter)Available sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pB6-Rosa26-cre_dre_reporter_1_2
Plasmid#185966PurposeB6J Rosa26-cre dre reporter 1.2 allele targeting vector, low copy numberDepositorInsertsfGFP, tdTomato
UseCre/Lox and Mouse Targeting; Dre/roxTagsExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB6-Rosa26-cre_dre_reporter_1_1b
Plasmid#185965PurposeB6J Rosa26-cre dre reporter 1.1b allele targeting vector, low copy numberDepositorInsertsfGFP, tdTomato
UseCre/Lox and Mouse Targeting; Dre/roxTagsExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB6-Rosa26-cre_dre_reporter_1_1a
Plasmid#185964PurposeB6J Rosa26-cre dre reporter 1.1a allele targeting vector, low copy numberDepositorInsertsfGFP, tdTomato
UseCre/Lox and Mouse Targeting; Dre/roxTagsExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB6-Rosa26-cre_dre_reporter_1_1
Plasmid#185963PurposeB6J Rosa26-cre dre reporter 1.1 allele targeting vector, low copy numberDepositorInsertEGFP, tdTomato
UseCre/Lox and Mouse Targeting; Dre/roxTagsExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPD152 Reporter Template v2
Plasmid#138752PurposeTemplate for COMET promoters, expresses EYFP reporter upon promoter activation. BsaI sites produce palindromic sticky ends.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_Donor/Reporter-COL4A3
Plasmid#130279PurposeCOL4A3 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A3 donor templateDepositorInsertmCherry-eGFP
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCLXEBR-pTF-Epo
Plasmid#114285PurposeTET-inducible expression of ErythropoietinDepositorInsertErythropoietin (Epo Rat)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCWXPGR-pTF-EpoRtm
Plasmid#114286PurposeTET-inducible expression of Erythropoietin receptor without cytoplasmic tailDepositorInsertErythropoietin Receptor (Epor Rat)
UseLentiviralTagsExpressionMutationdeletion of the cytoplasmic domainPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiR-report-hARPC2
Plasmid#112549Purpose3'UTR reporter assayDepositorInsertARPC2 (ARPC2 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
Ternary BAC Reporter
Plasmid#78220Purposesynthetic gene circuitDepositorInsertsynthetic gene circuit
UseTagsExpressionMutationPromoterAvailable sinceMay 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMIR-192-Reporter
Plasmid#71872PurposeMammalian expression vector for the analysis of miR-19α activityDepositorInsertReverse complementary sequence of miR-192
UseTagsfirefly luciferaseExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMIR-193a-5p-Reporter
Plasmid#71873PurposeMammalian expression vector for the analysis of miR-193a-5p activityDepositorInsertReverse complementary sequence of miR-193a-5p
UseTagsfirefly luciferaseExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
sgRNA1_pNeurog2-Luciferase reporter
Plasmid#64160PurposePhotoactivatable transcription system. Mammalian expression of sgRNA1 to target pNeurog2-Luciferase reporter.DepositorInsertsgRNA1 for pNeurog2-Luciferase reporter
UseCRISPRTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lentiviral-TOP-dGFP-reporter
Plasmid#14715PurposeBeta-catenin reporter - 3rd generation self-inactivating lentiviral plasmid LEF-1/TCF-responsive promoter driven d2-eGFP (destabilized, half-life of 2h).DepositorInsertLEF-1/TCF-responsive promoter (Ctnnb1 )
UseLentiviralTagsd2 eGFPExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
p53-TRE-mStrawberry reporter
Plasmid#158684PurposeThis plasmid is a p53 pathway reporter. It has two copies of wild-type p53 binding sites promoter driving the expression of mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
HIF-TRE-mStrawberry reporter
Plasmid#158681PurposeThis plasmid is a HIF1A pathway reporter. It has a 6 x HIF binding element promoter driving the expression of mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tet-inducible mCherry reporter
Plasmid#64128PurposePhotoactivatable transcription system. mCherry reporter under the control of a tet-inducible promoterDepositorInsertmCherry
UseTagsExpressionMammalianMutationPromoterTetOx13-CMVAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only