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pPtPuc3

Plasmid

#62863

Purpose

Episome vector for Phaeodactylum tricornutum (pUC-based)

Depositor

Hamilton Smith

Article

Karas et al Nat Commun. 2015 Apr 21;6:6925. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Episomal vector for phaeodacty…

Tags

Expression

Mutation

Promoter

Available since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Rheb

Plasmid

#19996

Depositor

Fuyuhiko Tamanoi

Article

Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

Insert

Ras homolog enriched in brain 1 (RHEB Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

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myc-mTOR (1750-2549 aa)

Plasmid

#21747

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

mTOR (1750-2549) (Mtor Rat)

Use

Tags

myc

Expression

Mammalian

Mutation

amino acids 1750-2549

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
pME18s-HA-hFIP200

Plasmid

#24303

Depositor

Noboru Mizushima

Article

Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

Insert

FIP200 (RB1CC1 Human)

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

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myc-mTOR (1271-2008 aa)

Plasmid

#21746

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

mTOR (1271-2008) (Mtor Rat)

Use

Tags

myc

Expression

Mammalian

Mutation

amino acids 1271-2008

Promoter

Available since

Aug. 18, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-AU1-mTOR-E2419K

Plasmid

#19994

Depositor

Fuyuhiko Tamanoi

Article

Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

Insert

mammalian target of rapamycin-E2419K (Mtor Rat)

Use

Tags

AU1

Expression

Mammalian

Mutation

Changed Glutamine 2419 to Lysine

Promoter

Available since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pAd-Track Flag-SIRT1

Plasmid

#8438

Depositor

Pere Puigserver

Article

Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

Insert

SIRT1 (Sirt1 Mouse)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pLVX Flag RagC WT

Plasmid

#112756

Purpose

express Flag-RagC

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagC (RRAGC Human)

Use

Lentiviral

Tags

Flag

Expression

Mutation

Promoter

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV14-mULK1

Plasmid

#24301

Depositor

Noboru Mizushima

Article

Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

Insert

a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-AU1-mTOR-L1460P

Plasmid

#19995

Depositor

Fuyuhiko Tamanoi

Article

Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

Insert

mammalian target of rapamycin-L1460P (Mtor Rat)

Use

Tags

AU1

Expression

Mammalian

Mutation

Changed Leucine 1460 to Proline

Promoter

Available since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pTn7·X-nia

Plasmid

#122592

Purpose

Plasmid for genomic integraction of xylS-Pm->nia into the Tn7 insertion site

Depositor

Pablo Ivan Nikel

Article

Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Insert

xylS/Pm->Nia

Use

Synthetic Biology; Tn7 genomic integration

Tags

Expression

Bacterial

Mutation

Promoter

Pm

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Raptor Halo

Plasmid

#112754

Purpose

express Raptor-Halo

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Raptor-Halo (RPTOR Human)

Use

Tags

Halo

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pKH161_FLAG-APEX2-YIPF3 WT

Plasmid

#214980

Purpose

Flag-APEX2 tagged YIPF3

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-APEX2-YIPF3 (YIPF3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pKH143_EGFP-YIPF3 LIR mut

Plasmid

#214975

Purpose

GFP tagged LIR mutant YIPF3

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

GFP-YIPF3_mut (YIPF3 Human)

Use

Lentiviral

Tags

Expression

Mutation

LIR mutant

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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SNF-5 pXOOM

Plasmid

#48697

Purpose

Expressed SNF-5 in Xenopus oocytes or mammalian cells

Depositor

Dmitri Boudko

Article

Metzler et al J Exp Biol. 2013 Aug 1;216(Pt 15):2843-5

Insert

snf-5 (snf-5 Nematode)

Use

Xenopus oocyte expression

Tags

Expression

Mammalian

Mutation

K42E (when compared to NP_496735.1)

Promoter

T7

Available since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pKH163_pSmart-mNEON-YIPF4

Plasmid

#214982

Purpose

endogenous mneon tag for YIPF4

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

mNEON with YIPF4 homology (YIPF4 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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PU.1-ets-IRES-mCherry

Plasmid

#80141

Purpose

retroviral expression of PU.1-ets (competitive inhibitor of PU.1) and mCherry

Depositor

Ellen Rothenberg

Article

Kueh et al Science. 2013 Aug 9;341(6146):670-3. doi

Insert

PU.1 (Spi1 Mouse)

Use

Retroviral

Tags

IRES-mCherry

Expression

Mammalian

Mutation

ets = PU.1 DNA binding domain (amino acids 159-26…

Promoter

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pAd-Track SIRT1 H355A

Plasmid

#8441

Depositor

Pere Puigserver

Article

Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

Insert

SIRT1 (Sirt1 Mouse)

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Histidine 355 mutated to Alanine

Promoter

Available since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only
-4200GAMT-P1-pGL4

Plasmid

#26804

Depositor

Sam Lee

Article

Ide et al Mol Cell. 2009 Nov 13. 36(3):379-92.

Insert

Guanidinoacetate N-methyltransferase (GAMT Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Jan. 6, 2011

Availability

Academic Institutions and Nonprofits only
pRK5 Flag RagB

Plasmid

#112755

Purpose

express Flag-RagB

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagB (RRAGB Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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