Addgene

We narrowed to 8,995 results for: sacs

Showing: 281 - 300 of 8995 results

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pSaCas9-sgRNA-Tetra-com vector

Plasmid

#131227

Purpose

For expressing gRNA, whose Tetraloop is replaced with a com aptamer

Depositor

Baisong Lu

Article

Lyu et al Nucleic Acids Res. 2019 Jul 12. pii: 553

Insert

vector for com modified sgRNA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pJEP302-pAAV-CMV-dSaCas9-KRAB-pA

Plasmid

#113677

Purpose

A CMV driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

de-catalyzed SaCas9

Use

AAV and CRISPR

Tags

KRAB and NLS

Expression

Mutation

Promoter

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgCnr1

Plasmid

#209196

Purpose

Mutagenesis of Cnr1

Depositor

Larry Zweifel

Article

Gunduz-Cinar et al Neuron. 2023 Jul 19:S0896-6273(23)00482-

Insert

Cnr1 (Cnr1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

CMV

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-pMecp2-dSaCas9-pU6-sgRNA

Plasmid

#159174

Purpose

Vector B encodes pAAV-pMecp2-dSaCas9-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of dSaCas9-sgRNA in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

pMecp2

Available since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pJEP305-pAAV-EFS-dSaCas9-KRAB-pA

Plasmid

#113681

Purpose

A EFS driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

de-catalyzed SaCas9

Use

AAV and CRISPR

Tags

KRAB and NLS

Expression

Mutation

Promoter

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pJEP303-pAAV-CMV-dSaCas9-VP64-pA

Plasmid

#113678

Purpose

A CMV driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted region

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

de-catalyzed SaCas9

Use

AAV, CRISPR, and Synthetic Biology

Tags

NLS and VP64

Expression

Mutation

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-SasgRNA scaffold-CMV-nSaCas9

Plasmid

#171695

Purpose

Expresses nSaCas9 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

Insert

sgRNA scaffold-nSaCas9-NLS

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A

Promoter

U6 and CMV

Available since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgSlc6a3

Plasmid

#159902

Purpose

Mutagenesis of Slc6a3

Depositor

Larry Zweifel

Insert

Slc6a3 (Slc6a3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCSD_1xNLS-SaCas9-1xNLS-3xHA-1xNLS

Plasmid

#107317

Purpose

Expresses SaCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SaCas9

Use

CRISPR

Tags

3xHA and 2xNLS and NLS

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEXfrt-SaCas9-U6-sgNtsr1

Plasmid

#159910

Purpose

Mutagenesis of Ntsr1

Depositor

Larry Zweifel

Insert

Ntsr1 (Ntsr1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgKcnj6

Plasmid

#159913

Purpose

Mutagenesis of Kcnj6

Depositor

Larry Zweifel

Insert

Kcnj6 (Kcnj6 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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p230-pOsAct1::hpt-pZmUbi::BdPetC

Plasmid

#129649

Purpose

Rieske FeS overexpression

Depositor

Susanne von Caemmerer

Article

Ermakova et al Commun Biol. 2019 Aug 16;2:314. doi: 10.

Insert

CDS of PetC gene encoding for the Rieske FeS subunit

Use

Tags

Expression

Plant

Mutation

Codon modified for Golden Gate

Promoter

pZmUbi

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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Coxsackievirus B3 (CVB3) 3C protease isolate KR-2002

Plasmid

#228639

Purpose

Bacterial expression of the Coxsackievirus 33 isoform B 3C protease

Depositor

ASAP Discovery, Frank von Delft

Insert

Coxsackievirus 33 isoform B 3C protease

Use

Tags

His-SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCMVtrunc beta-actinh7TAA-msfGFPΔN7ΔC11-SSSSactin

Plasmid

#231549

Purpose

Mammalian expression of human β-actin fused intramolecularly to N- and C-terminally truncated monomeric sfGFP, for actin filament organization measurements using fluorescence polarization

Depositor

Manos Mavrakis

Article

Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

Insert

ACTB (ACTB Human)

Use

Tags

msfGFP (intramolecular)

Expression

Mammalian

Mutation

Promoter

CMVtrunc

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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Anti-Fig4/Sac3 [N202/38R]

Plasmid

#220408

Purpose

Mammalian Expression Plasmid of anti-Fig4/Sac3 (Mouse) IgG2a R-mAb. Derived from hybridoma N202/38.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Fig4/Sac3 (Mus musculus) recombinant mouse monoclonal antibody. (Fig4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1312-AAV-EFSNC-dSaCas9-KRAB

Plasmid

#223157

Purpose

Expression of KRAB with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgFAAH

Plasmid

#209197

Purpose

Mutagenesis of Faah

Depositor

Larry Zweifel

Article

Balsevich et al Elife. 2023 Apr 11;12:e81919. doi: 10.75

Insert

Faah (Faah Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

CMV

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgKcnma1

Plasmid

#209199

Purpose

Mutagenesis of Kcnma1

Depositor

Larry Zweifel

Article

Juarez et al Sci Adv. 2023 Aug 11;9(32):eadg8869. doi

Insert

Kcnma1 (Kcnma1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

CMV

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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MsACR1-TS-Kv-P2A-mCerulean3-pAAV-CaMKII

Plasmid

#204970

Purpose

Soma-targeted neuronal expression of MsACR1 and mCerulean3 separated by a P2A site.

Depositor

Peter Hegemann

Article

Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

Insert

MsACR1

Use

AAV

Tags

mCerulean3

Expression

Mutation

Promoter

CaMKII

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pDRESS-mScarletI-antiFRET-P2A2-linker_noSacI

Plasmid

#191473

Purpose

Negative control for ratiometric comparison between green fluorescent proteins and sensors

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

none

Use

Tags

His-mScarletI-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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