pJEP304-pAAV-EFS-dSaCas9-VP64-pA
(Plasmid #113679)

• Purpose: A EFS driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted region
• Depositing Lab: Jonathan Ploski
• Publication: Kumar et al Front. Mol. Neurosci. (2018) 13

Backbone

• Vector backbone: AAV
  (Search Vector Database)
• Backbone manufacturer: Alligent Technologies
• Backbone size w/o insert (bp): 7073
• Total vector size (bp): 6739
• Vector type: AAV, CRISPR, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: de-catalyzed SaCas9
• Species: Synthetic
• Tags / Fusion Proteins:
  • VP64 (C terminal on insert)
  • NLS (N terminal on insert)
  • NLS (C terminal on insert)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: George Church, AAV_NLS-dSaCas9-NLS-VPR plasmid #68495
• Terms and Licenses:
  • UBMTA
• Industry Terms:
  • Not Available to Industry