pJEP304-pAAV-EFS-dSaCas9-VP64-pA
(Plasmid #113679)

• Purpose: A EFS driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted region
• Depositing Lab: Jonathan Ploski
• Publication: Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413. doi: 10.3389/fnmol.2018.00413. eCollection 2018.

Want a viral vector made from this plasmid?

Make a packaging request and we'll get back to you.

This service is available to academics and nonprofits only.

Please log in to submit a packaging request.

• Serotype: Select serotype for details See details about
• Pricing: Select serotype and quantity $ USD for preparation of µL virus + $30 USD for plasmid.
• How this works:
  • Place a request for a quantity of 10 (1 mL), 25 (2.5 mL), or 50 (5 mL). Our all-inclusive pricing includes DNA production and QC.
  • Addgene will quickly confirm that we can produce a high-quality prep for you.
  • Track your request and place an order from within your account. Payment information must be added before we can begin processing your order.
  • Receive your prep in 6–9 weeks after the MTA is approved by your organization.
  • Learn more about our Packaged on Request Service.

Backbone

• Vector backbone: AAV
• Backbone manufacturer: Alligent Technologies
• Backbone size w/o insert (bp): 7073
• Total vector size (bp): 6739
• Vector type: AAV, CRISPR, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: de-catalyzed SaCas9
• Species: Synthetic
• Tags / Fusion Proteins:
  • VP64 (C terminal on insert)
  • NLS (N terminal on insert)
  • NLS (C terminal on insert)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: George Church, AAV_NLS-dSaCas9-NLS-VPR plasmid #68495
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry