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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 2981 - 3000 of 18369 results

  1. MSCV Akt K179A mut

    Plasmid
    #18768
    Depositor
    Scott Lowe
    Insert
    Akt (AKT1 Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    K->A mutation
    Available Since
    July 11, 2008
    Availability
    Academic Institutions and Nonprofits only

  2. pET28-Nsp14-TEV-6His

    Plasmid
    #154952
    Purpose
    Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coli
    Depositor
    Justin Kollman
    Insert
    Nsp14-TEV-6His (ORF1ab SARS-CoV-2)
    Tags
    TEV-6His
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154952-plasmid-map-sequence-id-307820

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  3. pET28-6His-TEV-Nsp14

    Plasmid
    #154954
    Purpose
    Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable 6His tag in E. coli
    Depositor
    Justin Kollman
    Insert
    6His-TEV-Nsp14 (ORF1ab SARS-CoV-2)
    Tags
    6His-TEV
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154954-plasmid-map-sequence-id-307815

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  6. tdEos-Rad21-N-18

    Plasmid
    #57666
    Purpose
    Localization: Centromere, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Insert
    Rad21 (RAD21 Human)
    Tags
    tdEos
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57666-plasmid-map-sequence-id-233120

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  7. ECFP-Golgi-7

    Plasmid
    #55343
    Purpose
    Localization: Golgi, Excitation: 433, Emission: 475
    Depositor
    Michael Davidson
    Insert
    Golgi (B4GALT1 Human)
    Tags
    ECFP
    Expression
    Mammalian
    Mutation
    aa 1-82 of NM_001497.3
    Promoter
    CMV
    Available Since
    Oct. 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. mCherry-PTK2-N-10

    Plasmid
    #55123
    Purpose
    Localization: Focal Adhesion Kinase, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    PTK2 (PTK2 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  9. mEmerald-Keratin14-C-4

    Plasmid
    #54135
    Purpose
    Localization: Cytokeratin, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Keratin14 (KRT14 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 10, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54135-plasmid-map-sequence-id-456525

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  11. mEGFP-TPX2-N-10

    Plasmid
    #56289
    Purpose
    Localization: Centrosomes, Excitation: 488, Emission: 507
    Depositor
    Michael Davidson
    Insert
    TPX2 (TPX2 Human)
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 4, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. mPA-GFP-ER-3

    Plasmid
    #57131
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Insert
    ER (CALR Human)
    Tags
    mPA-GFP
    Expression
    Mammalian
    Mutation
    ER Signal Peptide
    Promoter
    CMV
    Available Since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. PA-mCherry1-H2B-C-10

    Plasmid
    #57181
    Purpose
    Localization: Histones/Nucleus, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Insert
    H2B (H2BC11 Human)
    Tags
    PA-mCherry1
    Expression
    Mammalian
    Mutation
    D26G and V119I in H2B
    Promoter
    CMV
    Available Since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    57181-plasmid-map-sequence-id-256477

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  14. mCherry-Tensin1-C-14

    Plasmid
    #55143
    Purpose
    Localization: Focal Adhesions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Tensin1 (TNS1 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. pcDNA3.1-MAP3K5 (R256C)

    Plasmid
    #47105
    Purpose
    Expresses mutant (R256C) human MAP3K5 in mammalian cells
    Depositor
    Yardena Samuels
    Insert
    MAP3K5 (MAP3K5 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    changed arginine 256 to cysteine
    Promoter
    CMV
    Available Since
    Oct. 31, 2013
    Availability
    Academic Institutions and Nonprofits only

  16. pGEX-6P-Cdh1wt (570)

    Plasmid
    #39877
    Depositor
    Jonathon Pines
    Insert
    FZR1 (FZR1 Human)
    Tags
    GST
    Expression
    Bacterial
    Available Since
    March 5, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. CAPN1

    Plasmid
    #25607
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CAPN1 (CAPN1 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids 29-360
    Available Since
    Oct. 25, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. pALS2-sfGFP 150TAG

    Plasmid
    #197574
    Purpose
    Fluorescence selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-150TAG and M. alvus Pyl-tRNA(6). p15a origin of replication.
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    sfGFP 150TAG
    M. alvus Pyl-tRNA (6)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    N150TAG
    Promoter
    araC and lpp
    Available Since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    197574-plasmid-map-sequence-id-388126

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  19. XLone-Puro SPI1 P2A mCherry

    Plasmid
    #140029
    Purpose
    Tunable and temporal expression control of SPI1 and mCherry
    Depositor
    Xiaoping Bao
    Insert
    SPI1 (SPI1 Human)
    Use
    Synthetic Biology
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140029-plasmid-map-sequence-id-275844

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  20. AICSDP-63: ACTN2-mEGFP

    Plasmid
    #124607
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human ACTN2, via excisable Cas9-excisable CAGGS-mCherry selection cassette
    Depositor
    Allen Institute for Cell Science
    Insert
    ACTN2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (ACTN2 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124607-plasmid-map-sequence-id-244336

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Showing: 2981 - 3000 of 18369 results