We narrowed to 8,512 results for: sgrna

1. hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector

   Plasmid

   #154430

   Purpose

   hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector

   Depositor

   David Liu

   Article

   Koblan et al Nature. 2021 Jan;589(7843):608-614. doi:

   Insert

   hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   VRQR point mutations in SpCas9

   Promoter

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJEP14-AAV-H1/TO-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

   Plasmid

   #82702

   Purpose

   AAV backbone with a minimal H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

   Depositor

   Jonathan Ploski

   Article

   de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

   Insert

   H1/TO Empty gRNA Expression Cassette
   

   Use

   AAV and CRISPR

   Tags

   CMV promoter driving expression of TetR-P2A-GFP-K…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJEP15-AAV-H1/TO-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

   Plasmid

   #82701

   Purpose

   AAV backbone with a minimal H1/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

   Depositor

   Jonathan Ploski

   Article

   de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

   Insert

   H1/TO gRNA Expression Cassette Containing Tet2 gRNA
   

   Use

   AAV and CRISPR

   Tags

   CMV promoter driving expression of TetR-P2A-GFP-K…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pW301-lenti-spsgRNA-hsITGB1-pEF1s-NLS-mNeonGreen-P2A-BlastR

   Plasmid

   #170817

   Purpose

   Lentiviral vector to co-express a human ITGB1 spsgRNA with NLS-mScarlet-I

   Depositor

   Kenneth Yamada

   Article

   Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

   Insert

   NLS-mNeonGreen-P2A-BlastR
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV hU6-VEGFR2#3 sgRNA Nestin-dCas9-KRAB-T2a-GFP

   Plasmid

   #196990

   Purpose

   Derived from pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP with KDR sgRNA

   Depositor

   Elizabeth Kirby

   Article

   Dause et al Mol Neurobiol. 2025 Mar 25. doi: 10.1007

   Insert

   VEGFR2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-MYL2-TadA8e-SpN aa2-713- inteinN-U6-Camk2d sgRNA7

   Plasmid

   #226915

   Purpose

   Expresses TadA8e and Sp cas9N by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

   Depositor

   Yuxuan Guo

   Article

   Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

   Insert

   MYL2, TadA, nSp Cas9N, inteinN
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   MYL2

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-MYL2-HA-TadA8e-Sauri N aa1-438-inteinN-U6-Camk2d sgRNA7

   Plasmid

   #226678

   Purpose

   Expresses TadA8e and Sauri cas9N by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

   Depositor

   Yuxuan Guo

   Article

   Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

   Insert

   MYL2, TadA, nSauri Cas9N, inteinN
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   MYL2

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-RnSyt1-sgRNA2-dCas9-KRAB-TagBFP2 (identifier AAAA-0246)

   Plasmid

   #202555

   Purpose

   Synaptotagmin-1 sgRNA2 with TagBFP2

   Depositor

   Frederic Meunier

   Article

   Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

   Insert

   CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCAGTACTCGCGTGCCTCGCACCGG) (Syt1 Rat)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-RnSyt1-sgRNA3-dCas9-KRAB-TagBFP2 (Identifier AAAA-0247)

   Plasmid

   #202556

   Purpose

   Synaptotagmin-1 sgRNA3 with TagBFP2

   Depositor

   Frederic Meunier

   Article

   Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

   Insert

   CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCTCCTCCTGCAGCGGCAGCATCGG) (Syt1 Rat)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-hUV6-sgRNA-dCas9-KRAB-TagBFP2 (identifier AAAA-0244)

    Plasmid

    #202553

    Purpose

    Nontargeting control vector with TagBFP2

    Depositor

    Frederic Meunier

    Article

    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

    Insert

    Humanized dCas9-KRAB-TagBFP2 (tag blue fluorescent protein 2) (TRIM28 S. Pyogenes (dCas9), H. sapiens (KRAB), Entacmaea quadricolor (TagBFP2))
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

    Expression

    Mutation

    Puromycin-resistance gene in the pLV hU6-sgRNA hU…

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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