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pGL403

Plasmid

#119953

Purpose

Expresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

Inserts

gpps
limS

Expression

Bacterial

Mutation

N-terminal truncation of 56 amino acid residues (…

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pExp-xMBP-ACE2-CHis

Plasmid

#194998

Purpose

Production of ACE2 extracellular domain

Depositor

Marko Hyvönen

Article

Harman et al J Med Chem. 2023 Jul 27;66(14):9881-9893

Insert

ACE2 (ACE2 Human)

Tags

His8 and xMBP

Expression

Bacterial

Promoter

T7lac

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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IL6R PLAK1

Plasmid

#161518

Purpose

Expresses IL6 receptor (full length) in mammalian cells.

Depositor

Yoav Shaul

Article

Khatib et al Proc Natl Acad Sci U S A. 2020 Sep 1;117

Insert

Interleukin 6 Receptor (IL6R Human)

Use

Lentiviral

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hPDGFRA-GFP

Plasmid

#22919

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

human platelet derived growth factor receptor alpha polypeptide promoter driving GFP (PDGFRA Human)

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pDRM166 LKB (Luciferase mKate2 Blast)

Plasmid

#183502

Purpose

Expresses the firefly luciferase gene, the NLS-tagged mKate2 gene, and the blasticidin resistance gene joined by P2A linkers.

Depositor

Wayne Tilley

Article

Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

Inserts

Firefly Luciferase
mKate2
BSD

Use

Lentiviral and Luciferase

Tags

P2A linker and SV40 NLS with GGS linker

Expression

Mammalian

Promoter

MND, MND (off Luc-P2A), and MND (off Luc-P2A-mKat…

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pET21a(+)-Bst LF-6xHis

Plasmid

#159148

Purpose

expresses His-tagged large fragment of Bst polymerase

Depositor

Andrea Pauli

Article

Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d

Insert

large fragment of Geobacillus stearothermophilus DNA polymerase I

Tags

His

Expression

Bacterial

Available Since

Sept. 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLX317-SNAI1

Plasmid

#115446

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

SNAI1 (SNAI1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.N

Plasmid

#127851

Purpose

non-standard AAV2 rep-AAV-PHP.N cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

Insert

Synthetic construct isolate AAV-PHP.N VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-FNLS-P2A-GFP-PGK-Puro

Plasmid

#110869

Purpose

Lentiviral vector for constitutive expression of FNLS-P2A-GFP in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-FNLS

Use

Lentiviral

Tags

3X FLAG

Mutation

D10A and NLS sequence at the N-terminus

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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CD44v-HA (pcDNA3)

Plasmid

#238417

Purpose

Expresses CD44, a single-pass transmembrane protein containing variable exons 3 to 10.

Depositor

Amy Shiu Lee

Article

Tseng et al Life Sci Alliance. 2019 Aug 15;2(4):e201

Insert

CD44 containing exons 3 to 10 (CD44 Human)

Tags

HA

Expression

Mammalian

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pSLIK_neo_3xflag_CHEK2

Plasmid

#136536

Purpose

Lentiviral expression vector for an inducible 3xflag-tagged CHEK2

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

CHEK2 (CHEK2 Human)

Use

Lentiviral; Destinatioin vector for gateway cloni…

Tags

3x FLAG

Expression

Mammalian

Available Since

June 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe-Puro-IKBalpha-mut (super repressor)

Plasmid

#15291

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

inhibitor of Kappa B alpha (NFKBIA Human)

Use

Retroviral

Expression

Mammalian

Mutation

S32A/S36A: “super-repressor” allele (resistant to…

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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pBabe-GFP-IKBalpha-mut (super repressor)

Plasmid

#15264

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

IKB alpha (NFKBIA Human)

Use

Retroviral

Expression

Mammalian

Mutation

Serine 32 to Alanine, Serine 36 to Alanine

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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Anti-TrpC4 [N77/15.1R]

Plasmid

#114504

Purpose

Mammalian Expression Plasmid of anti-TrpC4 (Rat). Derived from hybridoma N77/15.1.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-TrpC4 (Rattus norvegicus) recombinant mouse monoclonal antibody (Trpc4 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Neuroligin-3 [N110/29.4R]

Plasmid

#114518

Purpose

Mammalian Expression Plasmid of anti-Neuroligin-3 (Rat). Derived from hybridoma N110/29.4.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Neuroligin-3 (Rattus norvegicus) recombinant mouse monoclonal antibody (Nlgn3 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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Anti-Shank3 [N69/46R]

Plasmid

#140078

Purpose

Mammalian Expression Plasmid of anti-Shank3 (Rat). Derived from hybridoma N69/46.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Shank3 (Rat) recombinant mouse monoclonal antibody (Shank3 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pPRISM-Cre-cmlc2-mRFP

Plasmid

#117793

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

Cre

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-Cre-cmlc2-TagBFP

Plasmid

#117792

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

Cre

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Nav1.1 Na+ channel [K74/71R]

Plasmid

#128626

Purpose

Mammalian Expression Plasmid of anti-Nav1.1 Na+ channel (Rat). Derived from hybridoma K74/71.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Nav1.1 Na+ channel (Rat) recombinant mouse monoclonal antibody (Scn1a Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-VGlut3 [N34/34.2R]

Plasmid

#114494

Purpose

Mammalian Expression Plasmid of anti-VGlut3 (Rat). Derived from hybridoma N34/34.2.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-VGlut3 (Rattus norvegicus) recombinant mouse monoclonal antibody (Slc17a8 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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