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pL0_AC-lysA

Plasmid

#202117

Purpose

Level 0 plasmid containing the promoter from gene 21592 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

lysA promoter

Use

Synthetic Biology

Mutation

None

Available since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-NRT

Plasmid

#202115

Purpose

Level 0 plasmid containing the promoter from gene 54101 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

NRT promoter

Use

Synthetic Biology

Mutation

None

Available since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-sit4

Plasmid

#202114

Purpose

Level 0 plasmid containing the promoter from gene 48707 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

sit4 promoter

Use

Synthetic Biology

Mutation

None

Available since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-act1

Plasmid

#202113

Purpose

Level 0 plasmid containing the promoter from gene 29812 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

act1 promoter

Use

Synthetic Biology

Mutation

None

Available since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-CaMK2

Plasmid

#202112

Purpose

Level 0 plasmid containing the promoter from gene 39236 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

CaMK2 promoter

Use

Synthetic Biology

Mutation

None

Available since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-fbaC5

Plasmid

#202111

Purpose

Level 0 plasmid containing the promoter from gene 41423 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

fbaC5 promoter

Use

Synthetic Biology

Mutation

None

Available since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AB-OEE3

Plasmid

#202103

Purpose

Level 0 plasmid containing the promoter from gene 54499 from Phaeodactylum tricornutum with AB overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

OEE3 promoter

Use

Synthetic Biology

Mutation

None

Available since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AB-P49

Plasmid

#202102

Purpose

Level 0 plasmid containing the promoter from gene 49202 from Phaeodactylum tricornutum with AB overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

P49 promoter

Use

Synthetic Biology

Mutation

None

Available since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCAG- 3xFLAG-CtVPS13

Plasmid

#248330

Purpose

Mammalian expression of VPS13 from C. Thermophilum for in vitro studies

Depositor

Karin Reinisch

Publication

Li et al Mol Cell. 2026 Jul 7:S1097-2765(26)00417

Insert

VPS13

Tags

3xFLAG

Expression

Mammalian

Promoter

CAG

Available since

July 10, 2026

Availability

Academic Institutions and Nonprofits only

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pDD0921 pCS2-SPFMVp

Plasmid

#251792

Purpose

Potyviral protease specific against heptapeptide sequence: LEVYHQS

Depositor

Dave Dingal

Publication

Lim Suan et al Nat Commun 17, 3257 (2026).

Insert

SPFMVp

Expression

Mammalian

Promoter

CMV IE94

Available since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pDD0324 pCS2-PPVp

Plasmid

#251780

Purpose

Potyviral protease specific against heptapeptide sequence: NVVVHQA

Depositor

Dave Dingal

Publication

Lim Suan et al Nat Commun 17, 3257 (2026).

Insert

PPVp

Expression

Mammalian

Promoter

CMV IE94

Available since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pCHSU70.1-2.2

Plasmid

#110682

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Publication

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

PKS

Tags

flag

Expression

Yeast

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pYX-N-Puro-mNeon-WNK3

Plasmid

#222674

Purpose

endogenously tag WNK3 with N-terminal mNeon

Depositor

Jason Moffat

Publication

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

WNK3 (WNK3 Human)

Tags

mNeon

Expression

Bacterial

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pPW3783 : CMVd3-ERmembrane-GST-2xHaloTag-KKMP

Plasmid

#185687

Purpose

Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane, fused with a GST for constitutive dimerization (making a HaloTag tetramer).

Depositor

Peter Walter

Publication

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERmembrane-GST-2xHaloTag-KKMP

Expression

Mammalian

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-mTcte1-PA

Plasmid

#176469

Purpose

Expression vector of mouse t-complex-associated testis expressed 1 (Tcte1) tagged with PA at C-terminus.

Depositor

Masahito Ikawa

Publication

Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

Insert

t-complex-associated testis expressed 1 (Tcte1 Mouse)

Tags

PA

Expression

Mammalian

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EFIa-DIO-LMO3 (sbGLuc-VChR1-EYFP)

Plasmid

#114105

Purpose

Cre-dependent fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein (luminopsin LMO3) for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Insert

sbGLuc-VChR1-EYFP

Use

AAV

Expression

Mammalian

Promoter

EF1alpha

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgATL3-5

Plasmid

#109011

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Publication

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL3-sgRNA #5 (ATL3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

mU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCNL-N1_vinculin

Plasmid

#65691

Purpose

Expresses Vinculin-CNL in mammalian cells

Depositor

Yasushi Okada

Publication

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

vinculin (Vcl Mouse)

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only
pONL-N1_zyxin

Plasmid

#65683

Purpose

Expresses Zyxin-ONL in mammalian cells

Depositor

Yasushi Okada

Publication

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

zyxin (Zyx Mouse)

Use

Luciferase

Tags

ONL

Expression

Mammalian

Promoter

CMV

Available since

July 20, 2015

Availability

Academic Institutions and Nonprofits only
pONL-N1_TUBB5

Plasmid

#65680

Purpose

Expresses beta-tubulin-ONL in mammalian cells

Depositor

Yasushi Okada

Publication

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

beta-tubulin class V

Use

Luciferase

Tags

ONL

Expression

Mammalian

Promoter

CMV

Available since

June 18, 2015

Availability

Academic Institutions and Nonprofits only