We narrowed to 18,031 results for: Mut

1. pDEST40-ARL4D-V5-His6

   Plasmid

   #67447

   Purpose

   Mammalian expression of hArl4D with C-term V5 and His6 tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL4D (ARL4D Human)
   

   Use

   Tags

   His and V5

   Expression

   Mammalian

   Mutation

   bp 272 A to C; N91T mutation; bp 327 T to G; sil…

   Promoter

   CMV

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pET-DEST42-ARL4D-V5-His6

   Plasmid

   #67426

   Purpose

   Expression of hArl4D with C-term V5 and His6 tag in E. coli

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL4D (ARL4D Human)
   

   Use

   Tags

   His and V5

   Expression

   Bacterial

   Mutation

   bp 272 A to C; N91T mutation; bp 327 T to G; sil…

   Promoter

   T7/lacO

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pDEST47-ARL4D-GFP

   Plasmid

   #67405

   Purpose

   Mammalian expression of hArl4D with C-term GFP tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL4D (ARL4D Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   bp 272 A to C; N91T mutation; bp 327 T to G; sil…

   Promoter

   CMV

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pDONR221-ARL4D-no stop

   Plasmid

   #67337

   Purpose

   hArl4D without stop codon in Gateway Entry vector

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL4D (ARL4D Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Bacterial

   Mutation

   bp 272 A to C; N91T mutation; bp 327 T to G; sil…

   Promoter

   None

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA-HA-ER D538G

   Plasmid

   #49500

   Purpose

   Expresses HA-tagged ER D538G mutant in mammalian cells

   Depositor

   Sarat Chandarlapaty

   Article

   Toy et al Nat Genet. 2013 Dec;45(12):1439-45. doi:

   Insert

   ESR1 (ESR1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Aspartic acid 538 to Glycine

   Promoter

   CMV

   Available Since

   Dec. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA-HA-ER Y537S

   Plasmid

   #49499

   Purpose

   Expresses HA-tagged ER Y537S mutant in mammalian cells

   Depositor

   Sarat Chandarlapaty

   Article

   Toy et al Nat Genet. 2013 Dec;45(12):1439-45. doi:

   Insert

   ESR1 (ESR1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Tyrosine 537 to Serine

   Promoter

   CMV

   Available Since

   Dec. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3.1-FLAG-hSF3B1-K700E

   Plasmid

   #82577

   Purpose

   Expresses a codon-optimized ORF of human SF3B1 ( K700E mutant)

   Depositor

   Manoj Pillai

   Article

   Kesarwani et al Oncogene. 2016 Aug 15. doi: 10.1038/onc.

   Insert

   human SF3B1-K700E (SF3B1 Human)
   

   Use

   Tags

   FLAG peptide

   Expression

   Mammalian

   Mutation

   K700E

   Promoter

   CMV

   Available Since

   March 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Flag-IDH1-R132H

   Plasmid

   #62907

   Purpose

   Expresses R132 mutant IDH1 in mammalian cells

   Depositor

   Yue Xiong

   Article

   Zhao et al Science. 2009 Apr 10;324(5924):261-5. do

   Insert

   IDH1 (IDH1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Deleted Arg132 (R132)

   Promoter

   Available Since

   April 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

   Plasmid

   #182287

   Purpose

   Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Inserts

   codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
   PylT
   

   Use

   Tags

   nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   Y306A/Y384F (AF) double mutant of Methanosarcina …

   Promoter

   CMV and U6

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGM234

    Plasmid

    #220177

    Purpose

    Expresses exogenous wild-type NAC complex members (NACA and BTF3)

    Depositor

    Jonathan Weissman

    Article

    Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

    Insert

    NACA and BTF3 (NACA, BTF3 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG (NACA); 6xHis (BTF3)

    Expression

    Mutation

    Promoter

    Available Since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-C1-AR C619Y

    Plasmid

    #86429

    Purpose

    Fluorescent human androgen receptor (fused to EGFP) with point mutation C619Y

    Depositor

    Michael Mancini

    Article

    Nazareth et al Mol Endocrinol. 1999 Dec;13(12):2065-75.

    Insert

    Androgen receptor (AR) (AR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C619Y

    Promoter

    CMV

    Available Since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Negative Control Binder

    Plasmid

    #179140

    Purpose

    Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9) and an additionional A70Q mutation greatly reduces affinity for SsrA peptide

    Depositor

    Klaus Hahn

    Article

    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

    Insert

    SspB
    

    Use

    Tags

    FLAG and mCherry

    Expression

    Mammalian

    Mutation

    Y11K/A15E, residue numbering from pdb: 1ou9. Addi…

    Promoter

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Rb-379-928-pGex2TK

    Plasmid

    #21749

    Depositor

    William Kaelin

    Article

    Kaelin et al Cell. 1992 Jul 24. 70(2):351-64.

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deletion mutant ORF contains aa 379-928 of wt Rb …

    Promoter

    Available Since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUltra-Exon1Q23-Myc-B

    Plasmid

    #110488

    Purpose

    Lentiviral vector to express wild-type N terminal HTT gene with partial long 3'UTR in mammalian cells (encodes Myc-tagged wild-type huntingtin exon1 fragment with 23 repeats of Q)

    Depositor

    Baoji Xu

    Article

    Xu et al PLoS One. 2017 May 11;12(5):e0177610. do

    Insert

    Homo sapiens huntingtin (HTT Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    short 3'UTR mutant huntingtin exon1 fragment…

    Promoter

    UbC

    Available Since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1+FLAG-KrasnatG12D

    Plasmid

    #206842

    Purpose

    To express mouse Kras encoded by native mouse codons and with a G12D mutation with an N-terminal FLAG tag.

    Depositor

    Christopher Counter

    Article

    Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

    Insert

    Kras (Kras Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Glycine 12 to Aspartic Acid

    Promoter

    Available Since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. lenti-HA-mBec1 F121A

    Plasmid

    #99506

    Purpose

    Lentiviral expression of mutant Becn1 leading to hyperactive autophagy

    Depositor

    Congcong He

    Article

    Rocchi et al PLoS Genet. 2017 Aug 14;13(8):e1006962.

    Insert

    Beclin 1 (Becn1 Mouse)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    F121A

    Promoter

    CMV

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-FLEX-hKCNQ3(R230C)-P2A-EGFP

    Plasmid

    #209194

    Purpose

    Cre-dependent expression of Kcnq3 with R230C mutation and GFP from the same transcript

    Depositor

    Larry Zweifel

    Article

    Gamal El-Din et al Proc Natl Acad Sci U S A. 2021 Nov 9;118

    Insert

    Kcnq3 (KCNQ3 Human)
    

    Use

    AAV

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    R230 to C230

    Promoter

    CMV

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFB1.HMBP.PrS.dEZH2

    Plasmid

    #220241

    Purpose

    Expression of an EZH2 catalytic site mutant in insect cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    dEZH2 (EZH2 Human)
    

    Use

    Tags

    6x His, MBP

    Expression

    Insect

    Mutation

    Y731F

    Promoter

    Polyhedrin

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRRLNeo-pEF1a-p53ashL344A-mCerulean

    Plasmid

    #69580

    Purpose

    Expresses p53-L344A mutant tagged with mCerulean

    Depositor

    Galit Lahav

    Article

    Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

    Insert

    p53-L344A (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    p53 L344A mutation that prevents tetramerization …

    Promoter

    EF1alpha

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-ITR-PytR(PyOtR)-mCherry

    Plasmid

    #204870

    Purpose

    Expresses pyrrolysyl tRNA variant "PyOtR" and a wild-type mCherry reporter; can be packaged into AAV

    Depositor

    Abhishek Chatterjee

    Article

    Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202

    Insert

    M. mazei pyrrolysyl tRNA mutant "PyOtR"
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    U25C; A3G, A4C, A5G, C6G, G63C, T65G, T66C

    Promoter

    U6

    Available Since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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