We narrowed to 8,407 results for: reporter

1. pME-Actin-Vhh-sfGFP-P2A-1xHA-tdiRFP-caax (JDW 1311)

   Plasmid

   #224497

   Purpose

   Gateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and tdiRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Actin-Vhh-sfGFP-P2A-HA-tdiRFP-caax
   

   Use

   Gateway cloning

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-FLT3-D835N-V5/HIS

   Plasmid

   #236008

   Purpose

   expression of the D835N kinase defective mutant variant of human FLT3 that is associated with Acute Myeloid Leukemia

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human FLT3-D835N receptor tyrosine kinase, full length (FLT3 Human)
   

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   D835N substitution

   Promoter

   CMV

   Available Since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. ER-NFAST

   Plasmid

   #233596

   Purpose

   Expression of NFAST on the ER membrane

   Depositor

   Riccardo Filadi

   Article

   Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

   Insert

   NFAST
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. PM-RspA-CFAST

   Plasmid

   #233601

   Purpose

   Expression of RspA-CFAST on the PM facing the cytosol

   Depositor

   Riccardo Filadi

   Article

   Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

   Insert

   RspA-CFAST
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. OMM-short-RspA-NFAST

   Plasmid

   #233603

   Purpose

   Expression of RspA-NFAST on the outer mitochondrial membrane

   Depositor

   Riccardo Filadi

   Article

   Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

   Insert

   RspA-NFAST
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-TYRO3-V5/HIS

   Plasmid

   #200118

   Purpose

   Expression of human TYRO3 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human TYRO3 receptor tyrosine kinase (TYRO3 Human)
   

   Tags

   V5/His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. CaTCH Dual-sgRNA_sgBC1-A_sgBC1-C

   Plasmid

   #154196

   Purpose

   Lentiviral expression plasmid encoding two sgRNAs for targeting of the CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.

   Depositor

   Anna Obenauf

   Article

   Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

   Insert

   sgRNA-BC1-A, sgRNA-BC1-C
   

   Use

   Lentiviral; Catch barcode activation

   Expression

   Mammalian

   Promoter

   hU6 and mU6

   Available Since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-DDR2-V5/HIS

   Plasmid

   #201101

   Purpose

   expression of human DDR2 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human DDR2 receptor tyrosine kinase, full length, wildtype (DDR2 Human)
   

   Tags

   V5/His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-Sun1-2xsfGFP-6xMYC-pA (JDW 681)

   Plasmid

   #224489

   Purpose

   Gateway compatible middle entry clone containing Sun1 reporter fusion to 2 copies of sfGFP and 6 MYC tags (Nuclear envelope reporter and affinity tag)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Sun1-2xsfGFP-6xMYC-pA
   

   Use

   Gateway cloning

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV[Exp]-Puro-EF-1α>TurboRFP(ns):T2A:{H2B-RFP}

    Plasmid

    #201120

    Purpose

    RFP expression in cells under EF-1α promoter; Histone-bound, nuclear

    Depositor

    Ori Bar-Nur

    Article

    Zvick et al Stem Cell Reports. 2022 Sep 13;17(9):194

    Insert

    TurboRFP; H2B-RFP
    

    Use

    Lentiviral

    Mutation

    No

    Available Since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHL-H1-ccdB-mEF1a-RiH

    Plasmid

    #60601

    Purpose

    Cloning vector for CRISPR-sgRNA (into the BamHI-EcoRI site), expresses RFP and hygromycin resistance gene.

    Depositor

    Akitsu Hotta

    Article

    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

    Inserts

    Red Fluorescent Protein
    Hygromycin resistance gene
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. Tol2 8xSTAR-mNeonGreen. PGK-puro

    Plasmid

    #136255

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Oost et al Cell Rep. 2018 Feb 6;22(6):1600-1614. do

    Insert

    stem cell Ascl2 reporter, 8 repeats
    

    Use

    Tol2 transposase

    Tags

    mNeonGreen

    Expression

    Mammalian

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSBbi-RP-CoV2/RBD-puro

    Plasmid

    #161793

    Purpose

    Transposon-based SARS-CoV-2 RBD stable expression vector for protein production

    Depositor

    Jorge Luis Arias-Arias

    Article

    Arias-Arias et al Biotechnol Rep (Amst). 2023 Mar;37:e0078

    Insert

    SARS-CoV-2 RBD
    

    Use

    Transposon

    Tags

    6xHis and dTomato

    Expression

    Mammalian

    Promoter

    EF1a/RPBSA

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. CaTCH Dual-sgRNA_sgBC2-A_sgBC2-C

    Plasmid

    #154197

    Purpose

    Lentiviral expression plasmid encoding two sgRNAs without a target. These can be used as a off-target control for CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.

    Depositor

    Anna Obenauf

    Article

    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

    Insert

    sgRNA-BC2-A, sgRNA-BC2-C
    

    Use

    Lentiviral; Catch barcode activation

    Expression

    Mammalian

    Promoter

    hU6 and mU6

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiCas9-Venus

    Plasmid

    #70267

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 protein and Venus reporter from EFS promoter. Lentiviral backbone.

    Depositor

    Daniel Bauer

    Article

    Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature

    Inserts

    Cas9
    Venus Reporter
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EFS-NS and P2A

    Available Since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHL-EF1a-SphcCas9-iP-A

    Plasmid

    #60599

    Purpose

    Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

    Depositor

    Akitsu Hotta

    Article

    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

    Insert

    CRISPR Cas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Codon usage optimized for human usage

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. OMM-long-CFAST10

    Plasmid

    #233598

    Purpose

    Expression of CFAST10 on the OMM membrane after a long linker

    Depositor

    Riccardo Filadi

    Article

    Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

    Insert

    CFAST10
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCW57.1 _3xHA-Ago2

    Plasmid

    #73538

    Purpose

    Inducible lentiviral expression of Ago2

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    Ago2 (Ago2 Mouse)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet ON

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJOP-HTT-HR18Q

    Plasmid

    #87228

    Purpose

    HTT HR arms with 18 CAG repeats and piggyBac selection cassette

    Depositor

    Mahmoud Pouladi

    Article

    Xu et al Stem Cell Reports. 2017 Feb 21. pii: S22

    Insert

    1.7kb HTT 5' homology arm, 2.5kb HTT 3' homology arm
    

    Expression

    Mammalian

    Promoter

    EF1a driving selection cassette

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-miRFP-6xFLAG

    Plasmid

    #161614

    Purpose

    Cell morphology marker construct. Contains a near infrared fluorescent protein miRFP and six FLAG tags.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    miRFP-6xFLAG
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human synapsin 1 promoter

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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