We narrowed to 11,618 results for: Vars

1. pLEX-uORF-HA-birA*-N-Ras(wildtype)-IRES-Puro

   Plasmid

   #120563

   Purpose

   Expresses HA-birA*-N-Ras wildtype fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLEX-uORF-HA-birA*-N-Ras(Q61K)-IRES-Puro

   Plasmid

   #120564

   Purpose

   Expresses HA-birA*-N-Ras Q61K fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   Q61K

   Promoter

   CMV

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVITRO1-dV-IgG1/κ

   Plasmid

   #52213

   Purpose

   Human immunoglobulin gamma 1 antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   Human immunoglobulin gamma 1 constant region
   Human immunoglobulin kappa constant region
   

   Expression

   Mammalian

   Mutation

   Deleted Variable region

   Promoter

   Mouse Elongation Factor 1 Alpha and Rat Elongatio…

   Available Since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVITRO1-dV-IgE/κ

   Plasmid

   #61878

   Purpose

   Human immunoglobulin epsilon antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   Human immunoglobulin Epsilon constant region
   Human immunoglobulin kappa constant region
   

   Expression

   Mammalian

   Mutation

   Deleted Variable region

   Promoter

   mEF1 Prom and rEF1 Prom

   Available Since

   March 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3XFLAG-CMV-14-WDR5

   Plasmid

   #59974

   Purpose

   Mammalian Expression of WDR5-FLAG

   Depositor

   Debu Chakravarti

   Article

   Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

   Insert

   WDR5 (WDR5 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Oct. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVITRO1-dV-IgG1/λ

   Plasmid

   #52214

   Purpose

   Human immunoglobulin gamma 1 antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   Human immunoglobulin gamma 1 constant region
   Human immunoglobulin lambda constant region
   

   Expression

   Mammalian

   Mutation

   Deleted Variable region

   Promoter

   Mouse Elongation Factor 1 Alpha and Rat Elongatio…

   Available Since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVITRO1-dV-IgE/λ

   Plasmid

   #61879

   Purpose

   Human immunoglobulin epsilon antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   Human immunoglobulin Epsilon constant region
   Human immunoglobulin lambda constant region
   

   Expression

   Mammalian

   Mutation

   Deleted Variable region

   Promoter

   mEF1 Prom and rEF1 Prom

   Available Since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. B-Sniper SpCas9

   Plasmid

   #207361

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-Sniper SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   F539S, M763I, K890N, amino acids 1005-1013 replac…

   Promoter

   CBh

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. MAC-FURIN

   Plasmid

   #158386

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   furin, paired basic amino acid cleaving enzyme (FURIN Human)
   

   Tags

   MAC-tag

   Expression

   Mammalian

   Available Since

   Aug. 25, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pcDNA3_erGAP3

    Plasmid

    #78118

    Purpose

    Low affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulum

    Depositor

    Javier García-Sancho, Teresa Alonso

    Article

    Navas-Navarro et al Cell Chem Biol. 2016 Jun 1. pii: S2451-9

    Insert

    erGAP3
    

    Tags

    KDEL and calreticulin signal peptide

    Expression

    Mammalian

    Mutation

    S175G, D180Y, I167V and L15Q in GFP moiety and D…

    Promoter

    CMV

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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