We narrowed to 3,960 results for: LYC

1. 3021_pETcon-SARS-CoV-2-RBD_K417N_E484K_N501Y

   Plasmid

   #184406

   Purpose

   yeast surface display of the SARS-CoV-2 Beta variant RBD

   Depositor

   Jesse Bloom

   Article

   Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

   Insert

   SARS-CoV-2 Beta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
   

   Use

   Tags

   HA and c-Myc

   Expression

   Yeast

   Mutation

   K417N+E484K+N501Y

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. B-SpCas9-HF1

   Plasmid

   #126762

   Purpose

   Expression plasmid for human codon-opt. increased fidelity Blackjack-SpCas9-HF1 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than SpCas9HF1.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   B-SpCas9-HF1
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A; R661A; Q695A; Q926A; amino acids 1005-1013…

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. tRNA-gRNA position [2_n-1] (GB1206)

   Plasmid

   #75407

   Purpose

   tRNA and scaffold for the assembly of GBoligomers for the intermediate position (positon [2_n-1]) of a polycistronic tRNA-gRNA (3-part multiplexing)

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   tRNA-gRNA position [2_n-1]
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. AIDx-ID-1058

   Plasmid

   #135347

   Purpose

   Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 with uracil DNA glycosylase inhibitor (UGI) at the C terminus

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSpyCas9-hAIDx-ID-1058-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. C-His-GvpC-Calpain

   Plasmid

   #153295

   Purpose

   Expresses calpain-sensitive variant of Anabaena flos-aquae GvpC in E.coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

   Insert

   Calpain-sensitive Gas vesicle protein C (his-tagged)
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   1) Contains an extra glycine after the methionine…

   Promoter

   T7

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. delNC C-His Ana GvpC in pET28a

   Plasmid

   #85736

   Purpose

   Expresses a truncated His-tagged GvpC from Anabaena flos-aquae without the N-and C-terminal regions in E.coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

   Insert

   Gas vesicle protein C (truncated and his-tagged)
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Deletion of N and C terminal regions, addition of…

   Promoter

   T7

   Available since

   March 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. tRNA-gRNA position [D1_2] (GB1205)

   Plasmid

   #75406

   Purpose

   tRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_2]) of a polycistronic tRNA-gRNA regulated by the dicot U6-26 or U6-1 promoter (3-part multiplexing)

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   tRNA-gRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. B-eSpCas9

   Plasmid

   #126761

   Purpose

   Expression plasmid for human codon-optimized increased fidelity Blackjack-eSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than eSpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   B-eSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   K848A; K1003A; R1060A; amino acids 1005-1013 repl…

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. B-evoSpCas9

   Plasmid

   #126765

   Purpose

   Expression plasmid for human codon-opt. increased fidelity Blackjack-evoSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than evoSpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   B-evoSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   M495V; Y515N; K526E; R661Q; amino acids 1005-1013…

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pECE beta1 R760A

    Plasmid

    #50511

    Depositor

    Kenneth Yamada

    Article

    Pankov et al J Biol Chem. 2003 May 16;278(20):18671-8

    Insert

    integrin beta1 (ITGB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R760A

    Promoter

    SV40

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

11. RON6-bio

    Plasmid

    #47785

    Purpose

    Expresses enzymatically monobiotinylated full-length RON6 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised RON6
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin replaced…

    Promoter

    CMV

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR-2B-Nkx2-5 delta HD-G8

    Plasmid

    #98614

    Purpose

    Gateway entry vector NKX2-5 _HD

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    NKX2-5 _HD (Nkx2-5 Mouse)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Deletion of the homeodomain. Replaced by 8 glycin…

    Promoter

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNUT N6His Y95F/Y188F hTFNG

    Plasmid

    #69601

    Purpose

    Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in the N-lobe

    Depositor

    Ross MacGillivray, Anne Mason

    Article

    Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2

    Insert

    mutated human serum transferrin (TF Human)
    

    Use

    Tags

    Hexa His tag and N-terminal signal peptide, 4 aa …

    Expression

    Mammalian

    Mutation

    Asn413 Asp, Asn611Asp, Tyr95Phe, Tyr188Phe

    Promoter

    SV40

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-SARS-CoV-2-S-ectodomain

    Plasmid

    #235984

    Purpose

    Internally tagged SARS-CoV-2 spike ecto-domain

    Depositor

    Syed Saif Hasan

    Article

    Singh et al J Struct Biol X. 2025 Feb 11;11:100123.

    Insert

    Spike protein (S SARS-CoV-2)
    

    Use

    Tags

    8xHis and Twin-Strep tag and internal Twin-Strep …

    Expression

    Mammalian

    Mutation

    aa 1-1208, D614G, RRAR684-684GSAS, and DK987-987P…

    Promoter

    CMV

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-4T1_GST-LC3C

    Plasmid

    #223727

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    LC3C (MAP1LC3C Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 2-126 only, with Glycine exposed

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-4T1_GST-GBRP

    Plasmid

    #223728

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    GABARAP (GABARAP Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 2-116 only, with Glycine exposed

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Shim_GlyRS_87.19_SHI04113_TP_GFP_pK7fWG2_IVD_FP611

    Plasmid

    #228377

    Purpose

    Expresses N-term transit peptide from S. himalayana Glycine aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 87.19 (SHI04113RA); determination of Sapria himalayana GlyRS target localization

    Depositor

    Daniel Sloan

    Article

    DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    N-terminal transit peptide from S. himalayana GlyRS evm.model.fragScaff scaffold 87.19 (SHI04113RA)
    

    Use

    Tags

    Green Florescent Protien

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pdnCas3-CDA1

    Plasmid

    #229535

    Purpose

    pMV_hyg encoding dnCas3(H74A+D75A)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Inserts

    dnCas3
    Cytidine deaminase
    Uracil glycosylase inhibitor
    

    Use

    Tags

    XTEN

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae and codon optimi…

    Promoter

    Available since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pdnCas3-APOBEC1

    Plasmid

    #229537

    Purpose

    pMV_hyg encoding dnCas3(H74A+D75A)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Inserts

    rAPOBEC1
    dnCas3
    Uracil glycosylase inhibitor
    

    Use

    Tags

    XTEN

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae and codon optimi…

    Promoter

    Available since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-F-box:gs10:FRB:HA

    Plasmid

    #228531

    Purpose

    F-box domain of human Skp2 protein in fusion with FRB domain through a flexible glycine-serine linker.

    Depositor

    Roman Jerala

    Article

    Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

    Insert

    F-box:gs10:FRB:HA (SKP2 Synthetic, Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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