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pEAHISPA4631

Plasmid

#97017

Purpose

Expressing Pseudomonas aeruginosa orf PA4631 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Nucleoside-diphosphate-sugar epimerase

Tags

N-ter TEV protease cleavable 6HIs

Mutation

none

Promoter

T7

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISSSO2373

Plasmid

#97058

Purpose

Expressing Sulfolobus solfataricus orf SSO2373 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

S. solfataricus orf SSO2373

Tags

N-terminal TEV protease cleavable 6xHis tag

Mutation

none

Promoter

T7

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pEHISSSO1986

Plasmid

#97057

Purpose

Expressing Sulfolobus solfataricus orf SSO1986 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

S. solfataricus orf SSO1986

Tags

N-terminal TEV protease cleavable 6xHis tag

Mutation

none

Promoter

T7

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPApqsL

Plasmid

#97020

Purpose

Expressing Pseudomonas aeruginosa pqsL protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Putative FAD-dependent monooxygenase

Tags

N-ter TEV protease cleavable 6HIs

Mutation

none

Promoter

T7

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISMRSAPPFK

Plasmid

#97003

Purpose

Expressing MRSA PPFK protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Putative phosphofructokinase

Tags

N-ter TEV protease cleavable 6His

Mutation

none

Promoter

T7

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISSO1404

Plasmid

#97056

Purpose

Expressing Sulfolobus solfataricus orf SSO1404 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

S. solfataricus orf SSO1404

Tags

N-terminal TEV protease cleavable 6xHis tag

Mutation

none

Promoter

T7

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPSVORF239

Plasmid

#97025

Purpose

Expressing Pyrobaculum spherical virus orf239 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

PSV orf 239

Tags

N-ter TEV protease cleavable 6HIs

Mutation

none

Promoter

T7

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPSVORF165A

Plasmid

#97024

Purpose

Expressing Pyrobaculum spherical virus orf165a protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

PSV orf 165a

Tags

N-ter TEV protease cleavable 6HIs

Mutation

none

Promoter

T7

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPSVORF137

Plasmid

#97023

Purpose

Expressing Pyrobaculum spherical virus orf137 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

PSV orf 137

Tags

N-ter TEV protease cleavable 6HIs

Mutation

none

Promoter

T7

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISMRSA1376

Plasmid

#97002

Purpose

Expressing MRSA sar1376 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Putative 4-oxalocrotonate tautomerase

Tags

N-ter TEV protease cleavable 6His

Mutation

none

Promoter

T7

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPSVORF131

Plasmid

#97022

Purpose

Expressing Pyrobaculum spherical virus orf131 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

PSV orf 131

Tags

N-ter TEV protease cleavable 6HIs

Mutation

none

Promoter

T7

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPA4511

Plasmid

#97015

Purpose

Expressing Pseudomonas aeruginosa orf PA4511 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

P. aeruginosa orf PA4511

Tags

N-ter TEV protease cleavable 6HIs

Mutation

none

Promoter

T7

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISMRSATAG

Plasmid

#97004

Purpose

Expressing MRSA TAG protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

DNA-3-methyladenine glycosylase I

Tags

N-ter TEV protease cleavable 6His

Promoter

T7

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5-F-Med26-NTD- K74A K75A

Plasmid

#49252

Purpose

Mammalian expression of flag-tagged human Med26-NTD- K74A K75A

Depositor

Joan Conaway, Ronald Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

Med 26 (MED26 Human)

Tags

Flag

Expression

Mammalian

Mutation

K74A, K75A, aa 1-339

Available Since

Dec. 30, 2013

Availability

Academic Institutions and Nonprofits only
pLEX_305-C-dTAG

Plasmid

#91798

Purpose

Lentiviral/gateway cloning vector for C-terminally tagging proteins of interest with the dTAG system

Depositor

James Bradner, Behnam Nabet

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Type

Empty backbone

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Promoter

hPGK

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pDDGFP2_leu2d_NRT1.1

Plasmid

#58332

Purpose

Expresses Arabidopsis thaliana NRT1.1 in Saccharomyces cells as a C-terminal GFP his tagged protein

Depositor

Simon Newstead

Article

Parker et al Nature. 2014 Mar 6;507(7490):68-72. doi:

Insert

NRT1.1 (NRT1.1 Mustard Weed)

Tags

GFP-His

Expression

Yeast

Promoter

GAL1

Available Since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

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sUPRa

Plasmid

#223242

Purpose

sUPRa is a dual promoter and two-color construct, which is designed to report the global unfolded protein response (UPR) and affords unbiased cell detection irrespective of UPR levels

Depositor

Colin Akerman

Article

Chakrabarty et al Sci Rep. 2024 Jul 1;14(1):14990. doi: 10

Insert

sUPRa

Use

AAV

Promoter

Custom

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pLIX403_Capture1_APOBEC_HA_P2A_mRuby

Plasmid

#183901

Purpose

Inducible lentiviral expression, TRE-gateway-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

Depositor

Eugene Yeo

Article

Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

Type

Empty backbone

Use

Lentiviral; Gateway destination vector, doxycycli…

Tags

Capture1_APOBEC_HA_P2A_mRuby

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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CanlonicSF

Plasmid

#178342

Purpose

To explore lactate dynamics in intact systems

Depositor

Alejandro San Martín

Article

Aburto et al ACS Sens. 2022 Oct 28. doi: 10.1021/acss

Insert

CanlonicSF

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCas-CmR(+)

Plasmid

#113633

Purpose

Chloramphenicol resistant derivative of pCas plasmid

Depositor

Olivier Lichtarge

Article

Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

Insert

Chloramphenicol resistance gene

Use

CRISPR

Promoter

Pcat

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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