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p2T-CMV-evoAmax-BlastR

Plasmid

#152996

Purpose

C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

evoA-nSpCas9-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pSecEffector

Plasmid

#172891

Purpose

Contains TEVp display cassette, atc inducible (in any E. coli strain) BxbI and arabinose inducible TP901

Depositor

Urartu Seker

Article

Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

Inserts

Bxb1
TP901
TEV Display cassette
ProD promoter between anti aligned Bxb1 sites

Expression

Bacterial

Promoter

ProD, nTETO, and pBAD /AraC

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_VEGFB

Plasmid

#183328

Purpose

All-in-One CRISPRko system with a guide RNA that targets VEGFB gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

VEGFB

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_VEGFA

Plasmid

#183327

Purpose

All-in-One CRISPRko system with a guide RNA that targets VEGFA gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

VEGFA

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pFETCh_NFIL3

Plasmid

#86287

Purpose

Donor vector for 3' FLAG tag of human NFIL3

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

NFIL3 homology arms (NFIL3 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_NFIL3_2

Plasmid

#86349

Purpose

Encodes gRNA for 3' target of human NFIL3

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against NFIL3 (NFIL3 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_NFIL3_1

Plasmid

#86348

Purpose

Encodes gRNA for 3' target of human NFIL3

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against NFIL3 (NFIL3 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCDH_ss-SBP-EGFP

Plasmid

#172358

Purpose

Reporter to monitoring a streptavidin-binding peptide trafficking

Depositor

Guido Kroemer

Article

Zhao et al Sci Rep. 2018 Oct 8;8(1):14966. doi: 10.

Insert

ER importing signal sequence

Use

Lentiviral

Tags

SBP-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-Cas9-P2A-Puro_NFATC2-sgRNA

Plasmid

#188704

Purpose

Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human NFATC2

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

NFATC2 sgRNAs

Use

Lentiviral

Expression

Mammalian

Promoter

hUbC

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2 Flag Smad3 T179V

Plasmid

#26997

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

T179V

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
CROP_C9_Puro_GART

Plasmid

#183296

Purpose

All-in-One CRISPRko system with a guide RNA that targets GART gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

GART

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pTol2_(-3kb)nrg1_typeII:GFP-CAAX

Plasmid

#199394

Purpose

pDEL63; transgenic construct to express GFP-CAAX under nrg1 type II regulatory region control

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

(-3kb)nrg1 type II
GFP-CAAX
afp_3'_UTR

Use

Tol2 gateway destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCASP-HilA empty vector

Plasmid

#153333

Purpose

Triggers HilA overexpression from Salmonella upon induction with arabinose without any synthetic protein expressed

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Type

Empty backbone

Use

Salmonella expression

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pVPSB_empty

Plasmid

#192170

Purpose

pVPSB backbone expression vector

Depositor

Sidi Chen

Article

Yuan et al Mol Cell. 2020 Dec 17;80(6):1055-1066.e6

Type

Empty backbone

Expression

Mammalian

Mutation

NA

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pASH3 [pmyo-3::BeCNG1-YFP]

Plasmid

#168167

Purpose

Expression of BeCNG1-YFP in BWMs of C. elegans

Depositor

Alexander Gottschalk

Article

Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

Insert

BeCNG1-YFP

Tags

YFP

Expression

Worm

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pSUM-Kan-MCS1-gfp

Plasmid

#109288

Purpose

Expresses high levels of gfp in mycobacteria

Depositor

Nicolai van Oers

Article

Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

Insert

Multiple Cloning Site and GFP

Expression

Bacterial

Promoter

Kanamycin

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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pFETCh_MIXL1

Plasmid

#86280

Purpose

Donor vector for 3' FLAG tag of human MIXL1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

MIXL1 homology arms (MIXL1 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_TEAD3

Plasmid

#86281

Purpose

Donor vector for 3' FLAG tag of human TEAD3

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

TEAD3 homology arms (TEAD3 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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MD~E-pCLBabe

Plasmid

#20912

Depositor

Stephen Tapscott

Article

Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

Insert

MyoD~E12 forced dimer

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Short flexible linker region between the MyoD and…

Available Since

Nov. 2, 2009

Availability

Academic Institutions and Nonprofits only
pCS2 Flag Smad3 S208A

Plasmid

#27115

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

S208A

Available Since

Jan. 28, 2011

Availability

Academic Institutions and Nonprofits only