We narrowed to 6,531 results for: human c myc

1. p667-UBC-BRSK1-V5-miniTurbo_IDG-K

   Plasmid

   #135258

   Purpose

   Gateway destination clone of BRSK1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   BRSK1-V5-miniTurbo (BRSK1 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-miniTurbo

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. p667-UBC-BRSK1-V5_IDG-K

   Plasmid

   #135259

   Purpose

   Gateway destination clone of BRSK1 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   BRSK1-V5 (BRSK1 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p667-UBC-AAK1-V5_IDG-K

   Plasmid

   #135257

   Purpose

   Gateway destination clone of AAK1 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   AAK1-V5 (AAK1 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-RANGAP1-CTD

   Plasmid

   #228947

   Purpose

   express the C-terminal domain of the human RANGAP1 protein (residues 418-587 of UniProt P46060 with the N-terminal thrombin-cleavable MGSSHHHHHHSSGLVPR|GSHM- tag). Codon-optimized

   Depositor

   Marcin Suskiewicz

   Insert

   RANGAP1-CTD (RANGAP1 Human)
   

   Use

   Tags

   His tag and thrombine cleavage site : MGSSHHHHHHS…

   Expression

   Bacterial

   Mutation

   contains aa 418-587 only

   Promoter

   T7-lac

   Available since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-PE2-His (IK1822)

   Plasmid

   #170103

   Purpose

   T7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLS

   Depositor

   Keith Joung

   Article

   Petri et al Nat Biotechnol. 2021 Apr 29. pii: 10.103

   Insert

   bpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
   

   Use

   CRISPR

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Bacteria codon-optimized nSpCas9 (H840A) & M-…

   Promoter

   T7

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKK-BI16-TEV-mCherry

   Plasmid

   #105800

   Purpose

   Concomitant expression of two proteins. One protein is expressed with mCherry fusion tag (at the C-terminus), cleavable by TEV protease; second tag introduced by user.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKK-TEV-mCherry

   Plasmid

   #105796

   Purpose

   Expression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 15558047). mCherry descends from the first truly monomeric red FP mRFP1.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKK-TEV-mRuby3

   Plasmid

   #105797

   Purpose

   Expression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 26879144). mRuby3 is brighter than mCherry and has shifted ex and em spectra.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-mRuby3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKK-RNAi-nucEGFPmiR-TEV-mCherry

   Plasmid

   #105812

   Purpose

   Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with C-terminal mCherry.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   RNAi; Flp-in competent

   Tags

   TEV-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKK-RNAi-nucEGFPmiR-TEV-FLAG

    Plasmid

    #105811

    Purpose

    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with C-terminal FLAG.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    RNAi; Flp-in competent

    Tags

    TEV-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKK-RNAi-nucCHERRYmiR-TEV-FLAG

    Plasmid

    #105813

    Purpose

    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with C-terminal FLAG.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    RNAi; Flp-in competent

    Tags

    TEV-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKK-RNAi-nucCHERRYmiR-TEV-EGFP

    Plasmid

    #105814

    Purpose

    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with C-terminal EGFP.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    RNAi; Flp-in competent

    Tags

    TEV-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCA528 CHMP4B 156-224

    Plasmid

    #104596

    Purpose

    Expresses C-terminal 156-224 residues of CHMP4B with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-7

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP4B residues 156-224 (CHMP4B Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCA528 CHMP1B 143-199

    Plasmid

    #108255

    Purpose

    Expresses C-terminal 143-199 residues of CHMP1B with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-2

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP1B residues 143-199 (CHMP1B Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCA528 CHMP4B, residues 156-224, no Cys

    Plasmid

    #180641

    Purpose

    Bacterial expression for CHMP4B C-terminal tail, residues 156-224. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-140

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP4B (CHMP4B Human)
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 156-224

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. dPspCas13b-wtFTO

    Plasmid

    #223696

    Purpose

    Expressing fusion protein of dPspCas13b and wild type human FTO

    Depositor

    Chuan He

    Article

    Wei et al Science. 2022 May 27;376(6596):968-973.

    Insert

    dPspCas13b-GGS-wtFTO (FTO Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. dPspCas13b-mutFTO

    Plasmid

    #223697

    Purpose

    Expressing fusion protein of dPspCas13b and inactive mutant of human FTO

    Depositor

    Chuan He

    Article

    Wei et al Science. 2022 May 27;376(6596):968-973.

    Insert

    dPspCas13b-GGS-mutFTO (FTO Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBiT3.1-N_TLE1:1-770

    Plasmid

    #211248

    Purpose

    Mammalian expression of full-length human TLE1. N-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    TLE1:M1-Y770 (TLE1 Human)
    

    Use

    Tags

    MVSGWRLFKKISGSSGGSSG

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBiT3.1-N_STRAP:1-350

    Plasmid

    #211139

    Purpose

    Mammalian expression of full-length human STRAP. N-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    STRAP:M1-A350 (STRAP Human)
    

    Use

    Tags

    MVSGWRLFKKISGSSGGSSG

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBiT3.1-N_PRPF19:1-504

    Plasmid

    #211132

    Purpose

    Mammalian expression of full-length human PRPF19. N-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    PRPF19:M1-L504 (PRPF19 Human)
    

    Use

    Tags

    MVSGWRLFKKISGSSGGSSG

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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