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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 12,864 results for: sequence

Showing: 3101 - 3120 of 12864 results

  1. C35m

    Plasmid
    #120969
    Purpose
    MoClo golden gate assembly CD part for RhlR (Rhl quorum-sensing receptor for C4-HSL, synonymously modified to remove internal BbsI and BsaI cut sites).
    Depositor
    Richard Murray
    Insert
    RhlR
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BbsI sites removed; amino acid sequence …
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120969-plasmid-map-sequence-id-238056

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  2. pBS-2xpA

    Plasmid
    #176504
    Purpose
    2x SV40 polyA sequences
    Depositor
    Mario Capecchi
    Insert
    2x SV40 polyA
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176504-plasmid-map-sequence-id-348023

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  3. pBS-4xpA

    Plasmid
    #176506
    Purpose
    4x SV40 polyA sequences
    Depositor
    Mario Capecchi
    Insert
    4x SV40 polyA
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    176506-plasmid-map-sequence-id-347136

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  4. UC20m

    Plasmid
    #121040
    Purpose
    MoClo golden gate assembly DE part for gN2 gRNA (guide RNA for S. pyogenes Cas9; sequence designed with NUPACK for stable tracrRNA structure and open targeting region).
    Depositor
    Richard Murray
    Insert
    gN2 Cas9 gRNA UC part
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121040-plasmid-map-sequence-id-236353

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  5. pSpy

    Plasmid
    #29588
    Depositor
    Marc Fiedler
    Insert
    Spy (spy E.coli BL21(DE3))
    Use
    Tags
    Expression
    Bacterial
    Mutation
    processed form of Spy starting at residue 24 (Rem…
    Promoter
    Available since
    April 28, 2011
    Availability
    Academic Institutions and Nonprofits only

  6. pTUB:FLuc

    Plasmid
    #114578
    Purpose
    Constitutive expression of firefly luciferase in T. gondii using the alpha tubulin promoter; floxed pyrimethamine selectable marker; UPRT locus flanking sequence to direct integration
    Depositor
    David Sibley
    Insert
    Firefly Luciferase
    Use
    Cre/Lox and Luciferase
    Tags
    Expression
    Mutation
    Promoter
    alpha tubulin T. gondii
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    114578-plasmid-map-sequence-id-220131

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  7. mEmerald-Paxillin-KM-N-18

    Plasmid
    #54218
    Purpose
    Localization: Focal Adhesions, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Paxillin-KM
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Mutation to remove Kozak sequence
    Promoter
    CMV
    Available since
    July 10, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. mEmerald-Paxillin-KM-C-10

    Plasmid
    #54217
    Purpose
    Localization: Focal Adhesions, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Paxillin-KM
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Mutation to remove Kozak sequence
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. pCAGGS-MTS(S23A)-EGFP-V5

    Plasmid
    #200929
    Purpose
    expresses MTS(S23A)-EGFP-V5 in mammalian cells
    Depositor
    Hung-Chun Chang
    Insert
    EGFP
    Use
    Tags
    V5 and mitochondrial targeting sequence (MTS)
    Expression
    Mammalian
    Mutation
    MTS amino acid 23 mutated to Alanine
    Promoter
    CAG
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    200929-plasmid-map-sequence-id-410100

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  10. pCAGGS-MTS(S17/43A)-EGFP-V5

    Plasmid
    #200935
    Purpose
    expresses MTS(S17/43A)-EGFP-V5 in mammalian cells
    Depositor
    Hung-Chun Chang
    Insert
    EGFP
    Use
    Tags
    V5 and mitochondrial targeting sequence (MTS)
    Expression
    Mammalian
    Mutation
    S17A, S43A in MTS
    Promoter
    CAG
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    200935-plasmid-map-sequence-id-416912

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  11. pCAGGS-MTS(S6A)-EGFP-V5

    Plasmid
    #200817
    Purpose
    expresses MTS(S6A)-EGFP-V5 in mammalian cells
    Depositor
    Hung-Chun Chang
    Insert
    EGFP
    Use
    Tags
    V5 and mitochondrial targeting sequence (MTS)
    Expression
    Mammalian
    Mutation
    Changed Serine 6 to Alanine in MTS
    Promoter
    CAG
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    200817-plasmid-map-sequence-id-409561

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  12. pK2-J11

    Plasmid
    #218224
    Purpose
    Terminates AtU6-promoter driven sgRNA array with two guides, MoClo Compatible
    Depositor
    Daniel Voytas
    Insert
    linker sequence
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218224-plasmid-map-sequence-id-431992

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  13. pK2-K3

    Plasmid
    #218221
    Purpose
    Fuses Guides 1+2 to guides 3+4 in an of AtU6-promter driven sgRNAs
    Depositor
    Daniel Voytas
    Insert
    linker sequence
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    218221-plasmid-map-sequence-id-431988

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  14. pK4-J11

    Plasmid
    #218225
    Purpose
    Terminates AtU6-promoter driven sgRNA array with four guides, MoClo Compatible
    Depositor
    Daniel Voytas
    Insert
    linker sequence
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    218225-plasmid-map-sequence-id-431986

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  15. pK6-J11

    Plasmid
    #218226
    Purpose
    Terminates AtU6-promoter driven sgRNA array with tsix guides, MoClo Compatible
    Depositor
    Daniel Voytas
    Insert
    linker sequence
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    218226-plasmid-map-sequence-id-431985

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  16. pK4-K5

    Plasmid
    #218222
    Purpose
    Fuses Guides 3+4 to guides 6+6 in an of AtU6-promter driven sgRNAs
    Depositor
    Daniel Voytas
    Insert
    linker sequence
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    218222-plasmid-map-sequence-id-431983

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  17. pK8-J11

    Plasmid
    #218227
    Purpose
    Terminates AtU6-promoter driven sgRNA array with eight guides, MoClo Compatible
    Depositor
    Daniel Voytas
    Insert
    linker sequence
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    218227-plasmid-map-sequence-id-431982

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  18. pK6-K7

    Plasmid
    #218223
    Purpose
    Fuses Guides 5+6 to guides 7+8 in an of AtU6-promter driven sgRNAs
    Depositor
    Daniel Voytas
    Insert
    linker sequence
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    218223-plasmid-map-sequence-id-431979

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  19. pSiM2

    Plasmid
    #199381
    Purpose
    Encodes dual cistronic membrane localized GCaMP6f and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    GCaMP6f::ras-2CAAX::SL2::mScarlet-I::ras-2CAAX
    Use
    Tags
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Promoter
    Available since
    April 24, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199381-plasmid-map-sequence-id-391045

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  20. pAc5.1B-lambdaN-HA-DmYTHDFa_546-700_AB

    Plasmid
    #148302
    Purpose
    Insect Expression of DmYTHDFa_546-700
    Depositor
    Elisa Izaurralde
    Insert
    DmYTHDFa_546-700 (CG6422 Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    1 silent mutations compared to the sequence given…
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148302-plasmid-map-sequence-id-290750

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Showing: 3101 - 3120 of 12864 results