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pDONR223-PFKP

Plasmid

#23869

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PFKP (PFKP Human)

Use

Gateway donor vector

Mutation

1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pgWIZ-SGCAgl

Plasmid

#131456

Purpose

Encodes Alpha Sarcoglycan 3xFLAG tagged protein (Therapeutic gene) to cure LGMD2D and luciferase enzyme for in vivo live imaging

Depositor

Michele Calos

Article

Guha et al Mol Ther Methods Clin Dev. 2019 Oct 14;1

Insert

SGCA (SGCA Human)

Tags

3xFLAG

Expression

Bacterial and Mammalian

Available Since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-Dual-iCas9-CMV

Plasmid

#167500

Purpose

Expression of dual inducible Cas9 with CMV promoter

Depositor

Jake Liang

Article

Park et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

Insert

Cas9

Use

CRISPR, Lentiviral, and Luciferase

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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HIV-1 Gag-mCherry

Plasmid

#85390

Purpose

Visualization marker for viral core to measure cellular uptake

Depositor

Gregory Melikyan

Article

Miyauchi et al Biochem J. 2011 Mar 15;434(3):559-69. do

Insert

HIV-1 gag

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-sumo-h-cGAS

Plasmid

#127161

Purpose

expression of human cGAS protein

Depositor

Thomas Tuschl

Article

Lama et al Nat Commun. 2019 May 21;10(1):2261. doi:

Insert

MB21D1 (CGAS Human)

Tags

His-SUMO tag

Expression

Bacterial

Mutation

None

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pDisCoTune

Plasmid

#173713

Purpose

Promotes expression of disulfide-rich protein in E. coli T7 expression system. The plasmid encodes expression of the folding factors Erv1p and hPDI controlled by the Ptac promoter.

Depositor

Morten Norholm

Article

Bertelsen et al Microb Biotechnol. 2021 Nov;14(6):2566-2

Inserts

ERV1 (ERV1 Budding Yeast)
PDI

Expression

Bacterial

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA-FLAG-METTL3

Plasmid

#160250

Purpose

Express FLAG-METTL3 in human cells

Depositor

Alessandro Fatica

Article

Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

Insert

methyltransferase like 3 (METTL3 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-MFSD6-Puro

Plasmid

#239953

Purpose

Lentiviral vector to generate MFSD6 stable expressing cell line under CMV promoter

Depositor

Jan Carette

Article

Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

Insert

MFSD6

Use

Lentiviral

Tags

myc-flag

Expression

Mammalian

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp15-mNeongreen

Plasmid

#182389

Purpose

Bacterial expression of Vesicle Nucleating peptide15-mNeongreen fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp15-mNeongreen-His

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-EF1α-mKate2-biPAC

Plasmid

#169127

Purpose

AAV plasmid containing a mKate2 and a light-gated adenylate cyclase biPAC

Depositor

Georg Nagel

Article

Zhang et al Nature. 2021 Sep;597(7875):245-249. doi:

Insert

mKate2-biPAC

Use

AAV and Cre/Lox

Tags

mKate2

Expression

Mammalian

Mutation

codon optimized to mouse

Promoter

EF1a

Available Since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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