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pJK517

Plasmid

#71579

Purpose

Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Asp mutant (AarCH6-E435D)

Depositor

T. J. Kappock

Article

Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

Insert

succinyl-CoA:acetate CoA-transferase

Tags

His6

Expression

Bacterial

Mutation

changes glutamate-435 to aspartate

Promoter

T7

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pJK514

Plasmid

#71577

Purpose

Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Ala mutant (AarCH6-E435A)

Depositor

T. J. Kappock

Article

Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

Insert

succinyl-CoA:acetate CoA-transferase

Tags

His6

Expression

Bacterial

Mutation

changes glutamine-435 to alanine

Promoter

T7

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pJK512

Plasmid

#71575

Purpose

Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Arg228>Glu mutant (AarCH6-R228E)

Depositor

T. J. Kappock

Article

Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

Insert

succinyl-CoA:acetate CoA-transferase

Tags

His6

Expression

Bacterial

Mutation

changes arginine-228 to glutamate

Promoter

T7

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pES006 (pTet-qacR 2/pQacA-deGFP)

Plasmid

#69035

Purpose

reporter with qacR 2 on single plasmid

Depositor

Richard Murray

Article

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Inserts

qacR (qacR Synthetic)
deGFP

Tags

His6 tag

Expression

Bacterial

Mutation

E57Q, E58L, W61Y, E90Q, I99Q, M116Q, L119Y, E120Q…

Promoter

pQacA and pTet

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Shp2 T7DA/S189D SH2s

Plasmid

#67577

Purpose

Expresses GST-tagged Shp2 T73D/S189D SH2 domains in bacteria

Depositor

Graeme Carnegie

Article

Burmeister et al J Biol Chem. 2015 May 8;290(19):12058-67

Insert

Src homology 2 domain-containing phosphatase 2

Tags

GST

Expression

Bacterial

Mutation

Deleted amino acid residues 219-597, changed T73 …

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only
pVRa22_1147

Plasmid

#49675

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF22_1147

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available Since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pVRc22_1147

Plasmid

#49740

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

AS22_1147

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pLux

Available Since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pFSW Doc2alpha D291N mutant

Plasmid

#128813

Purpose

To make virus expressing Doc2alpha D291 mutant

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2alpha (Doc2a Rat)

Use

Lentiviral

Tags

IRES-EGFP

Mutation

D291N

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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pFSW Doc2beta D303N mutant

Plasmid

#128815

Purpose

To make virus expressing Doc2beta D303N mutant

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2beta (Doc2b Rat)

Use

Lentiviral

Tags

IRES-EGFP

Mutation

D303N

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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pFSW Doc2alpha Halotag

Plasmid

#128817

Purpose

To make virus expressing Doc2alpha Halotag

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2alpha (Doc2a Rat)

Use

Lentiviral

Tags

HaloTag-IRES-EGFP

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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pFSW Doc2beta Halotag

Plasmid

#128818

Purpose

To make virus expressing Doc2beta Halotag

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2beta (Doc2b Rat)

Use

Lentiviral

Tags

HaloTag-IRES-EGFP

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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MS2-adRNA (5'GAC)

Plasmid

#170127

Purpose

Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-ADAR2(5'GAC)-MS2

Use

Lentiviral

Expression

Mammalian

Promoter

Human U6 and mouse U6

Availability

Academic Institutions and Nonprofits only

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MS2-adRNA (3'GAC)

Plasmid

#170129

Purpose

Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-ADAR2(3'GAC)-MS2

Use

Lentiviral

Expression

Mammalian

Promoter

Human U6 and mouse U6

Availability

Academic Institutions and Nonprofits only

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SNX1-PX (142-269)

Plasmid

#119082

Purpose

Bacterial expression of human phox homology (PX) domain, SNX1-PX (142-269)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX1-PX (142-269) (SNX1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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SNX2-PX (136-279)

Plasmid

#119083

Purpose

Bacterial expression of human phox homology (PX) domain, SNX2-PX (136-279)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX2-PX (136-279) (SNX2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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SNX8-PX (70-190)

Plasmid

#119089

Purpose

Bacterial expression of human phox homology (PX) domain, SNX8-PX (70-190)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX8-PX (70-190) (SNX7 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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SNX15-PX (1-127)

Plasmid

#119096

Purpose

Bacterial expression of human phox homology (PX) domain, SNX15-PX (1-127)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX15-PX (1-127) (SNX15 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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SNX23-PX (1178-1312)

Plasmid

#119102

Purpose

Bacterial expression of human phox homology (PX) domain, SNX23-PX (1178-1312)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX23-PX (1178-1312)

Tags

GST

Expression

Bacterial

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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SNX24-PX (1-101)

Plasmid

#119103

Purpose

Bacterial expression of human phox homology (PX) domain, SNX24-PX (1-101)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX24-PX (1-101) (SNX24 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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PXK-PX (1-125)

Plasmid

#119114

Purpose

Bacterial expression of human phox homology (PX) domain, PXK-PX (1-125)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

PXK-PX (1-125) (PXK Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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