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pKR83:H2A1p-BD5-AS(50bp, 50%GC)2mm(18-19)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA

Plasmid

#251291

Purpose

Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mK2-Ex2

Expression

Mammalian

Promoter

H2A1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR85:H2A1p-BD7-AS (30bp)2mm(12-13)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA

Plasmid

#251293

Purpose

Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mK2-Ex2

Expression

Mammalian

Promoter

H2A1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR87:H2A1p-BD8-AS (30bp)2mm(12-13)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA

Plasmid

#251295

Purpose

Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mK2-Ex2

Expression

Mammalian

Promoter

H2A1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR89:H2A1p-BD9-AS (30bp)2mm(12-13)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA

Plasmid

#251297

Purpose

Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mK2-Ex2

Expression

Mammalian

Promoter

H2A1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR91:H2A1p-BD10-AS (30bp)2mm(12-13)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA

Plasmid

#251299

Purpose

Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mK2-Ex2

Expression

Mammalian

Promoter

H2A1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR71:H2A1p-(linker2)-AFP BD-AS(50bp)2mm(18-19)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA

Plasmid

#251283

Purpose

Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mK2-Ex2

Expression

Mammalian

Promoter

H2A1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR72:H2A1p-AFP BD-AS(50bp)2mm(18-19)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA

Plasmid

#251284

Purpose

Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mK2-Ex2

Expression

Mammalian

Promoter

H2A1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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puDNA5_SSF_MOR_2Ala_APEX2

Plasmid

#250760

Purpose

Mammalian cell expression of the mu opioid receptor with L387 and L390 mutated to alanines

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

MOR(2Ala)

Tags

APEX2 and Signal Sequence FLAG

Expression

Mammalian

Mutation

L387 and L400 are mutated to alanines

Promoter

UBC

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA5_TO_FRT_myc_GLUT4_2Ala_APEX2

Plasmid

#250767

Purpose

Mammalian cell expression of GLUT4 in which L500 and L503 are mutated to alanines

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

GLUT4(2Ala)

Tags

APEX2 and myc (in exofacial loop)

Expression

Mammalian

Mutation

L500 and L503 mutated to alanines

Promoter

CMV

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti_H1_VPS35_shRNA_UBC_SSF_SNAP_MOR_WT_P2A_GFP

Plasmid

#250765

Purpose

Mammalian cell expression of an shRNA against VPS35 and the mu opioid receptor

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

VPS35 shRNA

Tags

SSF-SNAP(for MOR)

Expression

Mammalian

Promoter

H1 for shRNA, UBC for MOR

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti_H1_Scramble_shRNA_UBC_SSF_SNAP_MOR_WT_P2A_GFP

Plasmid

#250764

Purpose

Mammalian cell expression of a scrambled shRNA and the mu opioid receptor

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

Scrambled VPS35 shRNA

Tags

SSF-SNAP (for MOR)

Expression

Mammalian

Promoter

H1 for shRNA, UBC for MOR

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti_SSF_MOR_2Ala_APEX2

Plasmid

#250774

Purpose

Mammalian cell expression of the mu opioid receptor with L387 and L390 mutated to alanines

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

MOR(2Ala)

Tags

APEX2 and Signal Sequence FLAG

Expression

Mammalian

Mutation

L387 and L400 are mutated to alanines

Promoter

UBC

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pN1_NUP62-EGFP3

Plasmid

#252748

Purpose

Expression of NUP62 with three tandem EGFPs.

Depositor

Kristen Verhey

Article

Takao et al Curr Biol. 2014 Oct 6;24(19):2288-94. do

Insert

NUP62 (Nup62 Mouse)

Tags

E3xGFP

Expression

Mammalian

Available Since

April 3, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST14-His6-TEV-SpyCatcher003-cys

Plasmid

#249543

Purpose

Expresses SpyCatcher003 with a N-terminal 6x-His tag and a C-terminal cysteine in E.coli under the T7 promoter

Depositor

Kai Zinn

Article

Anaya et al bioRxiv 2025.07.09.663943

Insert

SpyCatcher003-cys

Tags

6x His and Cysteine

Expression

Bacterial

Promoter

T7

Available Since

March 5, 2026

Availability

Academic Institutions and Nonprofits only

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pB-Orthogonal_LTR5Hs_CARGO

Plasmid

#248159

Purpose

Encodes a set of guide RNAs targeting LTR5Hs elements (orthogonal to Addgene #191316)

Depositor

Joanna Wysocka

Article

Fueyo et al Nature. 2025 Oct 1. doi: 10.1038/s41586-

Insert

Array of guide RNAs

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Available Since

Jan. 30, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1580_ECOR31HamAB_CoPurification

Plasmid

#223371

Purpose

T7 protein expression of MBP-His-TEV-ECOR31 Hachiman

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

MBP-His-TEV-ECOR31 Hachiman

Tags

MBP-His-TEV

Expression

Bacterial

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1370_pTet-ECOR31_Hachiman

Plasmid

#223364

Purpose

Expression of ECOR31 Hachiman under the pTet promoter

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

ECOR31 Hachiman

Expression

Bacterial

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1464_pTet-ECOR31Ham_DELhamA_Mut

Plasmid

#223365

Purpose

Expression of ECOR31 Hachiman HamB (∆HamA) under the pTet promoter

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

ECOR31 Hachiman HamB (∆HamA)

Expression

Bacterial

Mutation

∆HamA

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1465_pTet-ECOR31Ham_DELhamB_Mut

Plasmid

#223366

Purpose

Expression of ECOR31 Hachiman HamA (∆HamB) under the pTet promoter

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

ECOR31 Hachiman HamA (∆HamB)

Expression

Bacterial

Mutation

∆HamB

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1444_ECOR31HamA_purification

Plasmid

#223369

Purpose

T7 protein expression of MBP-His-TEV-ECOR31 Hachiman HamA

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

MBP-His-TEV-ECOR31 Hachiman HamA

Tags

MBP-His-TEV

Expression

Bacterial

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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