We narrowed to 4,369 results for: 1-Oct

1. pClneoFH-let60 G12V

   Plasmid

   #66860

   Purpose

   Express FLAG-His6-tagged dominant active let60, in which Glycine 12 is mutated to Valine, in mammalian cells

   Depositor

   Yutaka Hata

   Article

   Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

   Insert

   Let-60 (let-60 Nematode)
   

   Use

   Tags

   FLAG-His6

   Expression

   Mammalian

   Mutation

   Glycine 12 is mutated to valine

   Promoter

   CMV

   Available since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pClneoEGFPC-let60 K16N

   Plasmid

   #66861

   Purpose

   Express GFP-tagged dominat negative let60, in which Lysine 16 is mutated to asparagine, in mammalian cells

   Depositor

   Yutaka Hata

   Article

   Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

   Insert

   Let-60 (let-60 Nematode)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Lysine 16 is mutated to asparagine

   Promoter

   CMV

   Available since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

3. AbVec2.0-IGHG1

   Plasmid

   #80795

   Purpose

   Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG1 isotype

   Depositor

   Hedda Wardemann

   Article

   Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV IE1 gene promoter

   Available since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. AbVec1.1-IGKC

   Plasmid

   #80796

   Purpose

   Expression of immunoglobulin light chains in mammalian cells, human kappa constant region

   Depositor

   Hedda Wardemann

   Article

   Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV IE1 gene promoter

   Available since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRUSH

   Plasmid

   #54480

   Purpose

   Mouse genomic ROSA26 targeting vector, expresses EGFP, cre/lox conditional U6 promoter pgk-puro

   Depositor

   Laurie Jackson-Grusby

   Article

   Brown et al Genesis. 2014 Jan;52(1):39-48. doi: 10.1

   Type

   Empty backbone

   Use

   Cre/Lox, Mouse Targeting, and RNAi

   Tags

   SA-EGFP-polyA, U6 promoter, loxP, and pgk-puro

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pRUSH

   Plasmid

   #54479

   Purpose

   Mouse genomic ROSA26 targeting vector, expresses EGFP, U6promoter to drive short RNAs, pgkneo, cassette flanked by loxP sites

   Depositor

   Laurie Jackson-Grusby

   Article

   Brown et al Genesis. 2014 Jan;52(1):39-48. doi: 10.1

   Type

   Empty backbone

   Use

   Cre/Lox, Mouse Targeting, and RNAi

   Tags

   SA-EGFP-polyA, U6 promoter, loxP, and pgk-neo

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pCAGGS-V5-Scip

   Plasmid

   #225110

   Purpose

   Scip expression in Chick

   Depositor

   Polyxeni Philippidou

   Article

   Kc et al Commun Biol. 2024 Sep 17;7(1):1166. doi:

   Insert

   Scip (Pou3f1 Mouse)
   

   Use

   Avian expression

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDGO186N-KS1

   Plasmid

   #174301

   Purpose

   Replicating plasmid with sgRNA cassette containing a killing spacer #1 targeting the wild type polC gene.

   Depositor

   Lee Lynd

   Article

   Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

   Insert

   spacer expression cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBA2kIKmA2

   Plasmid

   #39213

   Depositor

   Anastasios Melis

   Article

   Lindberg et al Metab Eng. 2010 Jan;12(1):70-9. Epub 200

   Insert

   Kudzu Isoprene Synthase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Deleted the predicted chloroplast transit peptide

   Promoter

   psbA2

   Available since

   Aug. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pYS1

    Plasmid

    #117488

    Purpose

    Generation of unmarked gene deletions in Mycobacterium smegmatis

    Depositor

    Eyal Gur

    Article

    Shenkerman et al Gene. 2014 Jan 1;533(1):374-8. doi: 10.1

    Insert

    Che9c 60–61, sacB, aph
    

    Use

    Deletion generation

    Tags

    Expression

    Mutation

    Promoter

    Acetamide

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLEW100cre-EP1

    Plasmid

    #33345

    Purpose

    For expression of tetracycline-inducible CRE in Trypanosoma brucei

    Depositor

    George Cross

    Article

    Scahill et al Mol Biochem Parasitol. 2008 Jan;157(1):7

    Insert

    cre recombinase
    

    Use

    Cre/Lox; Trypanosoma brucei expression vector

    Tags

    Expression

    Mutation

    Promoter

    TET operator

    Available since

    Dec. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDH008

    Plasmid

    #157643

    Purpose

    sMHT-113 fused to CheY only

    Depositor

    Jonathan Silberg

    Article

    Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

    Inserts

    sMHT(1-113)
    CheY-sMHT(1-114)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. RBD-Fc

    Plasmid

    #164221

    Purpose

    Expresses soluble , dimeric Fc-His fusion protein for the original SARS-CoV2 Spike RBD region, along with dTomato reporter

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

    Insert

    Spike RBD (S SARS-CoV)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFastBac Dual α1B/β2B Tubulin

    Plasmid

    #170553

    Purpose

    Encodes codon-optimized human α1B and β2B tubulin for expression in and purification from insect cells.

    Depositor

    Tarun Kapoor

    Article

    Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

    Inserts

    TUBA1B (TUBA1B Human)
    TUBB2B (TUBB2B Human)
    

    Use

    Tags

    AP linker, GS linker (GGSGG), His10 tag, L21 enha…

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. IL1RN gRNA2

    Plasmid

    #113131

    Purpose

    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFastBac Dual α1B/β3 Tubulin

    Plasmid

    #170552

    Purpose

    Encodes codon-optimized human α1B and β3 tubulin for expression in and purification from insect cells.

    Depositor

    Tarun Kapoor

    Article

    Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

    Inserts

    TUBA1B (TUBA1B Human)
    TUBB3 (TUBB3 Human)
    

    Use

    Tags

    AP linker, GS linker (GGSGG), His10 tag, L21 enha…

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p2xFlag CMV2-YAP2-1st WW mutant

    Plasmid

    #19046

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

    Insert

    Yes-kinase associated protein isoform 3 (YAP1 Human)
    

    Use

    Tags

    2xFlag

    Expression

    Mammalian

    Mutation

    Tryptophan 199 changed to Alanine,and Proline 202…

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p2xFlag CMV2-YAP2-2nd WW mutant

    Plasmid

    #19047

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Use

    Tags

    2xFlag

    Expression

    Mammalian

    Mutation

    Tryptophan 258 changed to Alanine, and Proline 2…

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pENTR221-JUN

    Plasmid

    #79512

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human JUN CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    JUN (JUN Human)
    

    Use

    Multisite gateway entry clone

    Tags

    HA Tag

    Expression

    Mutation

    Promoter

    no Promoter

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO-TRE-AFOS

    Plasmid

    #197079

    Purpose

    A doxycycline inducible dominant negative form of transcription complex AP1. AFOS heterodimerizes and inhibits c-Jun. Plasmid contains PGK driven H2B-RFP as a fluorescent reporter of transduction.

    Depositor

    Elaine Fuchs

    Article

    Larsen et al Cell Stem Cell. 2021 Oct 7;28(10):1758-1

    Insert

    AFOS (Fos Mouse)
    

    Use

    Lentiviral

    Tags

    Flag and T7

    Expression

    Mutation

    Promoter

    TRE

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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