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pHACK-QF2

Plasmid

#80274

Purpose

QF2 HACK backbone. Contains QF2-hsp70, but no gRNAs

Depositor

Christopher Potter

Article

Lin et al Genetics. 2016 Jun 22. pii: genetics.116

Insert

QF2

Expression

Insect

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pAc5.1C-FLuc-Stop-5BoxB

Plasmid

#21301

Depositor

Elisa Izaurralde

Article

Rehwinkel et al RNA. 2005 Nov . 11(11):1640-7.

Insert

5BoxB

Use

Drosophila expression

Available Since

June 23, 2009

Availability

Academic Institutions and Nonprofits only

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pKDM

Plasmid

#110740

Purpose

Plasmid for the insect cell expression system, two promoters, Multiplication Module M, Tn7 transposition

Depositor

Tim Richmond

Article

Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

Type

Empty backbone

Expression

Insect

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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TJF229

Plasmid

#132575

Purpose

pZE1-oxySp-mCherry-proD-oxyR

Depositor

Timothy Lu

Article

Muller et al Nat Commun. 2019 Sep 6;10(1):4028. doi:

Inserts

oxySp-mCherry-TermT1
proD-oxyR-TermT1

Expression

Bacterial

Promoter

oxySp and proD

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pSK23

Plasmid

#72851

Purpose

template for C-terminal eGFP tagging, Puromycin-resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

eGFP

Expression

Insect

Promoter

copia

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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370 pTol2-lmo2:CherryDest

Plasmid

#73489

Purpose

Tol2 vector with lmo2 promoter upstream of Cherry, attR1/R2 cassette, and late SV40 pA signal

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

lmo2 promoter:mCherry-attR1-attR2

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pAPA3

Plasmid

#24777

Depositor

Tanya Parish

Article

Parish et al J Bacteriol. 2007 May . 189(10):3721-8.

Type

Empty backbone

Use

Integrating vector

Expression

Bacterial

Available Since

Aug. 4, 2010

Availability

Academic Institutions and Nonprofits only

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pMS2-1

Plasmid

#220627

Purpose

Plasmid used to place the RNA sequence of interest next to two MS2 coat protein recognition sites.

Depositor

Marvin Wickens

Article

Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pBRIT TAP

Plasmid

#17520

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Type

Empty backbone

Use

Cre/Lox and Retroviral

Tags

TAP

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
8xLexA-binding elements/pMD20

Plasmid

#48807

Purpose

Contains 8xLexA-binding elements

Depositor

Hodaka Fujii

Article

Fujita et al PLoS One. 2011;6(10):e26109. doi: 10.137

Insert

8xLexA-binding elements

Available Since

Dec. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pET28-a(+)-6xHis-LSSmCherry1(-13)

Plasmid

#219415

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Article

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Insert

LSSmCherry1(-13)

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

K137E, K199E

Promoter

T7

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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hHpr1-GST

Plasmid

#11200

Depositor

Robin Reed

Article

Masuda et al Genes Dev. 2005 Jul 1. 19(13):1512-7.

Insert

Hpr1 (THOC1 Human)

Tags

GST

Expression

Bacterial

Available Since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only
pMT-P4

Plasmid

#120866

Purpose

Encodes permuteposon P4 for MuA transposase-mediated circular permutation

Depositor

Jonathan Silberg

Article

Atkinson et al Methods Enzymol. 2019;621:191-212. doi:

Insert

Permuteposon P4

Use

Synthetic Biology

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsPAN2_M

Plasmid

#146941

Purpose

Mammalian Expression of HsPAN2

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsPAN2 (PAN2 Human)

Expression

Mammalian

Available Since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pMT-P2

Plasmid

#120864

Purpose

Encodes permuteposon P2 for MuA transposase-mediated circular permutation

Depositor

Jonathan Silberg

Article

Atkinson et al Methods Enzymol. 2019;621:191-212. doi:

Insert

Permuteposon P2

Use

Synthetic Biology

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pBS hBAF170

Plasmid

#17875

Depositor

Jerry Crabtree

Article

Wang et al Genes Dev. 1996 Sep 1. 10(17):2117-30.

Insert

BAF170 (SMARCC2 Human)

Expression

Bacterial

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
pMT-P3

Plasmid

#120865

Purpose

Encodes permuteposon P3 for MuA transposase-mediated circular permutation

Depositor

Jonathan Silberg

Article

Atkinson et al Methods Enzymol. 2019;621:191-212. doi:

Insert

Permuteposon P3

Use

Synthetic Biology

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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371 pTol2-fli1ep:MTDest

Plasmid

#73490

Purpose

Tol2 vector with fli1ep element upstream of 6xmyc, attR1/R2 cassette, and late SV40 pA signal

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

fli1ep:6xMyc-attR1-attR2

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pSK41

Plasmid

#74886

Purpose

template for insertion of attP site (C-terminal tag plasmid design), Puromycin-Resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

attP site

Expression

Insect

Available Since

April 28, 2016

Availability

Academic Institutions and Nonprofits only

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pJAS1052

Plasmid

#158598

Purpose

VioA, VioB

Depositor

John Dueber

Article

Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

Inserts

VioA
VioB

Expression

Yeast

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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