We narrowed to 5,339 results for: pcdna3.1

1. pcDNA3 Plk4(Sak) D154A (Nigg HR26)

   Plasmid

   #41164

   Depositor

   Erich Nigg

   Article

   Habedanck et al Nat Cell Biol. 2005 Nov;7(11):1140-6.

   Insert

   Plk4 D154A (PLK4 Human)
   

   Tags

   3xMyc and PreScission Site

   Expression

   Mammalian

   Mutation

   D154A

   Promoter

   CMV

   Available Since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-Golgi-ZapCY1

   Plasmid

   #36322

   Depositor

   Amy Palmer

   Article

   Qin et al Proc Natl Acad Sci U S A. 2011 May 3;108

   Insert

   Golgi-ZapCY1 high affinity Zn(II) sensor
   

   Tags

   GalT (first 60 amino acids)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 31, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. mNeonGreen-IETD-NanoLuc

   Plasmid

   #98288

   Purpose

   caspase-8 BRET Sensor

   Depositor

   Maarten Merkx

   Article

   den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

   Insert

   mNeonGreen-LEHD-NanoLuc
   

   Tags

   His-tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCD/NL-BH*deltavpu/RT-

   Plasmid

   #136985

   Purpose

   Second-generation lentiviral packaging plasmid lacking reverse transcriptase activity

   Depositor

   Jakob Reiser

   Article

   Zhang et al J Virol. 2004 Feb . 78(3):1219-29.

   Insert

   HIV-1 Gag, Pol (Integrase), Tat, Rev, Vpr, Vif
   

   Expression

   Mammalian

   Mutation

   carries D110E mutation in the reverse transcripta…

   Available Since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-LBR-mRFP1-polyA

   Plasmid

   #59749

   Purpose

   Express LBR-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   LBR-mRFP1
   

   Tags

   mRFP1

   Expression

   Mammalian

   Mutation

   aa 1-249 of LBR

   Promoter

   CMV and T7

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. FRB-myc

   Plasmid

   #20228

   Depositor

   Carolyn Bertozzi

   Article

   Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

   Insert

   FRB (MTOR Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   T2098L point mutation

   Available Since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pCMV_mCherry_2A_HA_2xMS2-VP64

   Plasmid

   #68419

   Purpose

   Transient expression of humanized MS2 (V75E/A81G), fused to the VP64 transcrption activator, in mammalian cells, under a CMV promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   MS2
   

   Use

   Synthetic Biology

   Tags

   HA and VP64

   Expression

   Mammalian

   Mutation

   MS2: V75E/A81G

   Promoter

   CMV

   Available Since

   Sept. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cenp-B INCENP GFP

   Plasmid

   #45238

   Purpose

   Target INCENP to centromeres via full length CENP-B

   Depositor

   Michael Lampson

   Article

   Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

   Insert

   Cenp-B, INCENP-Δcen, GFP
   

   Tags

   GFP and full length CENP-B

   Expression

   Mammalian

   Mutation

   CEN box deleted from INCENP (aa1-46)

   Available Since

   Sept. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. VinculinTS-A50I/I997A

   Plasmid

   #111829

   Purpose

   Vinculin tension sensor with point mutations affecting ability to bind talin and actin

   Depositor

   Brenton Hoffman

   Article

   Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

   Insert

   VinculinTS (I997A)
   

   Expression

   Mammalian

   Mutation

   changed vinculin alanine 50 to isoleucine and iso…

   Promoter

   CMV

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. miniCMV-iRFP670-tDeg

    Plasmid

    #185403

    Purpose

    iRFP670-tDeg fluorogenic protein

    Depositor

    Jian-Hui Jiang

    Article

    Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

    Insert

    iRFP670-tDeg
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOVC1_TtCarH-CBD_X

    Plasmid

    #131179

    Purpose

    Expresses TtCarH-CBD (Thermus termophilus) followed by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    TtCarH-CBD
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOVC1_MxCarH-CBD_X

    Plasmid

    #131178

    Purpose

    Expresses MxCarH-CBD (Myxococcus xanthus) followed by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    MxCarH-CBD
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVH607-Tier1-PCREm4-Nluc

    Plasmid

    #169594

    Purpose

    Tier-1 vector encoding PCREm4-driven Nluc expression (PCREm4-Nluc-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    cAMP response element-controlled NanoLuc Luciferase expression cassette
    

    Expression

    Mammalian

    Promoter

    PCREm4

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Mito-LSSmGFP

    Plasmid

    #186536

    Purpose

    Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to mitochondria

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    LSSmGFP
    

    Tags

    Human COX8a first 29 amino acids

    Expression

    Mammalian

    Mutation

    hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

    Promoter

    CMV

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. tCRMod-GGSGGS9

    Plasmid

    #111762

    Purpose

    Expresses soluble Clover-mRuby2 tension sensor module with 9-repeat GGSGGS extensible domain

    Depositor

    Brenton Hoffman

    Article

    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

    Insert

    Clover-GGSGGS9-mRuby2 fusion
    

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available Since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVH231-Tier1-PmPGK1-mTagBFP2

    Plasmid

    #169520

    Purpose

    Tier-1 vector encoding PmPGK1-driven mTagBFP2 expression (PmPGK1-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven monomeric Tag blue fluorescent protein 2
    

    Expression

    Mammalian

    Promoter

    PPGK

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. ER2-mGreenLantern

    Plasmid

    #186548

    Purpose

    Localization and retention of mGreenLantern fluorescent protein in the ER; improved targeting efficiency

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    mGreenLantern
    

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CMV

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. ER2-LSSmGFP

    Plasmid

    #186538

    Purpose

    Localization and retention of LSSmGFP fluorescent protein (ex/em = 400/510 nm) in the ER; improved targeting efficiency

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    LSSmGFP
    

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Mutation

    hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

    Promoter

    CMV

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPD005 pcDNA Golden Gate

    Plasmid

    #138749

    Purpose

    Vector used to express most COMET components, modified from pcDNA3.1/HygroR(+) for BsaI/BpiI Golden Gate compatibility and to lack HygroR.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-ALFA-tag-Gal4-VP16

    Plasmid

    #199465

    Purpose

    Expression of the Gal4-ALFA-tag-VP16 synthetic transcription factor containing the ALFA-tag sequence in mammalian cells.

    Depositor

    Vladislav Verkhusha

    Article

    Chernov et al Int J Mol Sci. 2023 Mar 30;24(7):6526. d

    Insert

    Gal4-ALFA-tag-VP16
    

    Expression

    Mammalian

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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