We narrowed to 8,512 results for: sgrna

1. pK2-J11

   Plasmid

   #218224

   Purpose

   Terminates AtU6-promoter driven sgRNA array with two guides, MoClo Compatible

   Depositor

   Daniel Voytas

   Insert

   linker sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pK2-K3

   Plasmid

   #218221

   Purpose

   Fuses Guides 1+2 to guides 3+4 in an of AtU6-promter driven sgRNAs

   Depositor

   Daniel Voytas

   Insert

   linker sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pK4-J11

   Plasmid

   #218225

   Purpose

   Terminates AtU6-promoter driven sgRNA array with four guides, MoClo Compatible

   Depositor

   Daniel Voytas

   Insert

   linker sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pK6-J11

   Plasmid

   #218226

   Purpose

   Terminates AtU6-promoter driven sgRNA array with tsix guides, MoClo Compatible

   Depositor

   Daniel Voytas

   Insert

   linker sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pK4-K5

   Plasmid

   #218222

   Purpose

   Fuses Guides 3+4 to guides 6+6 in an of AtU6-promter driven sgRNAs

   Depositor

   Daniel Voytas

   Insert

   linker sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pK8-J11

   Plasmid

   #218227

   Purpose

   Terminates AtU6-promoter driven sgRNA array with eight guides, MoClo Compatible

   Depositor

   Daniel Voytas

   Insert

   linker sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pK6-K7

   Plasmid

   #218223

   Purpose

   Fuses Guides 5+6 to guides 7+8 in an of AtU6-promter driven sgRNAs

   Depositor

   Daniel Voytas

   Insert

   linker sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9(BB)-2A-Neo

   Plasmid

   #127762

   Purpose

   Used for expression of sgRNA in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSEVA47_dCas9-GFP_YES_i2

   Plasmid

   #231419

   Purpose

   This YES-gate plasmid expresses dCas9 from a constitutive promoter, GFP from a promoter repressible by sgRNA-Y, and sgRNA-Y repressible by sgRNA-2.

   Depositor

   Manish Kushwaha

   Article

   Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

   Inserts

   dCas9
   GFP
   sgRNA-Y
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGG369

    Plasmid

    #165606

    Purpose

    Vector for expression of ω-dCas9 and sgRNA in E. coli: lacUV2-ω-1xFLAG-dCas9(Wt PID)-1xNLS-3xHA-1xNLS and UV5-BsaI-sgRNA (BsaI sites in place of the spacer)

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    ω-1xFLAG-dCas9(PAM-interacting domain and overall coding scheme from Wt SpCas9)-1xNLS-3xHA-1xNLS and BsaI-sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    1x FLAG, 3x HA, Omega (ω) subunit of RNA polymera…

    Expression

    Bacterial

    Mutation

    Recoding of BsaI site in the AmpR cassette, two B…

    Promoter

    lacUV2 driving ω-dCas9 and UV5 driving sgRNA

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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