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sgRNA TR KO 2

Plasmid

#207610

Purpose

sgRNA 2 to knockout TR by replacement with a PuroR cassette

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

TCAGGCCGCAGGAAGAGGAA

Tags

None

Expression

Mammalian

Promoter

CMV and U6

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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sgRNA TR KO 1

Plasmid

#207609

Purpose

sgRNA 1 to knockout TR by replacement with a PuroR cassette

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

ACCCTAACTGAGAAGGGCGT

Tags

None

Expression

Mammalian

Promoter

CMV and U6

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pEN_TT NPIPB11-FLAG

Plasmid

#192308

Purpose

Gateway entry vector for an inducible NPIPB11-FLAG

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

NPIPB11 (NPIPB11 Human)

Use

Gateway Cloning

Tags

FLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT mCh-V5-NES-EGFP-mCBP80

Plasmid

#192313

Purpose

Gateway entry vector for an inducible mCh-V5-NES-EGFP-mCBP80

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

mCh-V5-NES-EGFP-mCBP80

Use

Gateway Cloning

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT mCh-V5-NES-EGFP-IBB

Plasmid

#192314

Purpose

Gateway entry vector for an inducible mCh-V5-NES-EGFP-IBB

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

mCh-V5-NES-EGFP-IBB

Use

Gateway Cloning

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pIM45

Plasmid

#193539

Purpose

pENTRY clone for NLR Integrated Domain ID#39

Depositor

Nemo Peeters

Article

Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

Insert

ID#39

Use

Gateway Cloning

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pXCX-sGFAP-DLDH-IRES-tdTomato

Plasmid

#178311

Purpose

Express the bacterial D-lactate dehydrogenase (DLDH) enzyme in astrocytes

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

Insert

D-lactate dehydrogenase

Use

Adenoviral

Tags

tdTomato

Expression

Mammalian

Promoter

sGFAP

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pChd4EosHaloTAF_Puro

Plasmid

#172099

Purpose

Targeting construct to make Chd4-Eos-Halo-Tev-Avi-3xFlag fusion

Depositor

Brian Hendrich

Insert

chromodomain helicase DNA binding protein 4 (Chd4 Mouse, Synthetic)

Use

Mouse Targeting

Tags

3xFLAG, Avi, Eos, Halo, and TEV

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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TagBFP-DiB2-splitN1–125

Plasmid

#168973

Purpose

N-fragment of the DiB2‐split protein fused with blue fluorescent protein TagBFP

Depositor

Jens Meiler

Article

Bozhanova et al Sci Rep. 2020 Jul 6;10(1):11049. doi: 10

Insert

TagBFP - DiB2-split N-frag

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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H2B-DiB2-splitN1–125

Plasmid

#168972

Purpose

N-fragment of the DiB2‐split protein fused with histone H2B

Depositor

Jens Meiler

Article

Bozhanova et al Sci Rep. 2020 Jul 6;10(1):11049. doi: 10

Insert

H2B - DiB2-split N-frag

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET11a-N-DiB2

Plasmid

#168472

Purpose

N-fragment of the DiB2‐split protein. This plasmid needs to be co-transformed with Plasmid 168257: pMRBad-C-wtBlc to obtain DiB2‐split protein

Depositor

Jens Meiler

Article

Bozhanova et al Sci Rep. 2020 Jul 6;10(1):11049. doi: 10

Insert

N-fragment of DiB2

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pLNL1141

Plasmid

#160867

Purpose

PJ23110-RBS-PhCFAT-mRuby2-tL3S2P24_KanR_p15A

Depositor

John Dueber

Article

Latimer et al ACS Synth Biol. 2020 Sep 18. doi: 10.102

Insert

Coniferyl alcohol acyltransferase

Use

Synthetic Biology

Tags

mRuby2

Expression

Bacterial

Promoter

PJ23110

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLNL1127

Plasmid

#160859

Purpose

PJ23110-RBS-RhAAT1-mRuby2-tL3S2P24_KanR_p15A

Depositor

John Dueber

Article

Latimer et al ACS Synth Biol. 2020 Sep 18. doi: 10.102

Insert

Acetyl CoA geraniol/citronellol acetyltransferase

Use

Synthetic Biology

Tags

mRuby2

Expression

Bacterial

Promoter

PJ23110

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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p8208 MSCV-Neo C-HA EMPTY

Plasmid

#163310

Purpose

Empty vector control

Depositor

Elizabeth White

Article

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Type

Empty backbone

Use

Retroviral

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-rab38a

Plasmid

#109699

Purpose

Multisite gateway vector for 3' tagging with rab38a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab38a (rab38a Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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PLKO.1-UPF3A-3'UTR

Plasmid

#136042

Purpose

UPF3A shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (GACGTAGAAACACGCAGAAAC)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF3A (UPF3A Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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PLKO.1-UPF3A-CDS

Plasmid

#136043

Purpose

UPF3A shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (GCAGAAACCATTCTAAAGAAA)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF3A (UPF3A Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pC0.040

Plasmid

#119584

Purpose

Level 0 part. Promoter

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

J23110MH

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 SCAF8_isoD_CRISPR_resistant

Plasmid

#122468

Purpose

pDONR for Gateway cloning

Depositor

Jesper Svejstrup

Article

Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

Insert

SCAF8 isoform D CRISPR resistant (SCAF8 Human)

Use

Gateway Cloning

Mutation

Synonymous mutations conferring resistance to gRN…

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 SCAF4_CRISPR resistant

Plasmid

#122465

Purpose

pDONR for Gateway cloning

Depositor

Jesper Svejstrup

Article

Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

Insert

SCAF4 CRISPR resistant (SCAF4 Human)

Use

Gateway Cloning

Mutation

Synonymous mutations conferring resistance to gRN…

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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