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Green Lindoblum

Plasmid

#163113

Purpose

Expresses biosensor for lactate in mammalian cells

Depositor

Takashi Tsuboi

Article

Harada et al Sci Rep. 2020 Nov 11;10(1):19562. doi: 1

Insert

Green Lindolum

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-JEDI-2Psub-WPRE

Plasmid

#247180

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-2Psub in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKIIa

Depositor

Francois St-Pierre

Article

Carolan et al Nat Commun. 2025 Oct 3;16(1):8834. doi:

Insert

JEDI-2Psub

Use

AAV

Expression

Mammalian

Promoter

CaMKIIa

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-RhoA

Plasmid

#23224

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

RhoA (RHOA Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
Green Pegassos

Plasmid

#163114

Purpose

Expresses biosensors for pyruvate in mammalian cells

Depositor

Takashi Tsuboi

Article

Harada et al Sci Rep. 2020 Nov 11;10(1):19562. doi: 1

Insert

Green Pegassos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pRD461

Plasmid

#178198

Purpose

Rok with a C-terminal His-tag overexpression

Depositor

Remus Dame

Article

Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

Insert

rok 6xHis

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-EGFP-anti-rabbit-IgG(Fc)-nanobody

Plasmid

#138133

Purpose

Expresses GST-tagged EGFP-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

EGFP-anti-rabbit-IgG(Fc)-nanobody

Tags

GST

Expression

Bacterial

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-mCherry-anti-rabbit-IgG(Fc)-nanobody

Plasmid

#138131

Purpose

Expresses GST-tagged mCherry-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

mCherry-anti-rabbit-IgG(Fc)-nanobody

Tags

GST

Expression

Bacterial

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-PGAL1-3HA

Plasmid

#41608

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

3xHA, A. gossypii translation elongation factor 1…

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pDEST-V1-ORF

Plasmid

#73635

Purpose

Gateway™ destination vector encoding N-terminal fusion with Venus fluorescent protein fragment 1 (V1).

Depositor

Darren Saunders

Article

Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

Type

Empty backbone

Tags

Venus fragment 1 (V1)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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mTFP1-H2A-10

Plasmid

#55488

Purpose

Localization: Nucleus/Histones, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

H2A (H2AC13 Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pDEST-V2-ORF

Plasmid

#73636

Purpose

Gateway™ destination vector encoding N-terminal fusion with Venus fluorescent protein fragment 2 (V2).

Depositor

Darren Saunders

Article

Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

Type

Empty backbone

Tags

Venus fragment 2 (V2)

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn RSET-T-GECO1.WPRE

Plasmid

#211902

Purpose

Expresses T-GECO1 in mammalian cells

Depositor

Robert Campbell

Article

Aggarwal et al Neurophotonics. 2024 Apr;11(2):024207. d

Insert

T-GECO1

Use

AAV

Tags

RSET peptide

Expression

Mammalian

Promoter

hSyn

Available Since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pGL2- Empty control -SV40-Luc

Plasmid

#26280

Purpose

Addgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.

Depositor

Rudolf Jaenisch

Article

Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Available Since

Oct. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pET28a-FKBP-TRIF(Nterm)

Plasmid

#221334

Purpose

Overexpression of TRIF Nterm domain in bacteria

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF Nterm domain (Ticam1 Mouse)

Tags

6xHis-4xFKBP

Expression

Bacterial

Promoter

T7

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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Diffusive 67kDa

Plasmid

#201338

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

EGFP-6PrA

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCI-syn-fRGECO1

Plasmid

#125243

Purpose

Expresses red fluorescent calcium sensor in hippocampal slices

Depositor

Katalin Torok

Article

Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

Insert

fRGECO1

Expression

Mammalian

Mutation

D323A

Promoter

synapsin

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pMJA167

Plasmid

#128536

Purpose

Expresses DsRed

Depositor

Marcos Alcocer

Article

Wang et al Mol Biotechnol. 2014 Feb;56(2):136-46. d

Insert

NFAT-DsRed reporter

Expression

Mammalian

Promoter

SV40-NFAT

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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LZF1827 pAAV_hSyn-Flp-off-Cre-on-stGtACR2-CFP

Plasmid

#135413

Purpose

expresses GtACR2 linked to CFP

Depositor

Adam Cohen

Article

Fan et al Cell. 2020 Jan 23. pii: S0092-8674(20)30

Insert

stGtACR2-CFP

Use

AAV

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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9X NFAT-apha-MHC-Luc

Plasmid

#51941

Purpose

9 copies of NFAT sites in minimal alphaMHC-pGL-3

Depositor

Jeffery Molkentin

Article

Wilkins et al Circ Res. 2004 Jan 9;94(1):110-8. Epub 2

Inserts

9x NFAT binding site
alpha-MHC

Use

Luciferase

Tags

luciferase

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-NES-fRCaMP2

Plasmid

#125250

Purpose

Expresses red fluorescent calcium sensor in mammalian cells

Depositor

Katalin Torok

Article

Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

Insert

fRCaMP2

Expression

Mammalian

Mutation

D396A

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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