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Plasmid#183644PurposeTo (ectopically) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; mammalian expressionDepositorInsertATP6AP1 (ATP6AP1 Human)
UseTagsmycExpressionMammalianMutationtruncated ATP6AP1 mutant; contains only amino aci…PromoterCMVAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hAP1 Δ420-440
Plasmid#183642PurposeTo (stably) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; lentiviral expressionDepositorInsertATP6AP1 (ATP6AP1 Human)
UseLentiviralTagsHAExpressionMutationtruncated ATP6AP1 mutant lacking the transmembran…PromoterCMVAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
CXCR4-SZ158a QTY
Plasmid#162442PurposeExpression in E coli and perform ligand binding in solutionDepositorInsertC-X-C chemokine receptor type 4 (CXCR4 Human)
UseTagshis-tag, Rho-tagExpressionBacterialMutationAll L, I, V, F in the transmembrane region mutate…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
CXCR4-SZ146a QTY
Plasmid#162443PurposeExpression in E coli and perform ligand binding in solutionDepositorInsertC-X-C chemokine receptor type 4 (CXCR4 Human)
UseTagshis-tag, Rho-tagExpressionBacterialMutationAll L, I, V, F in the transmembrane region mutate…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
CXCR4-SZ158a-sfGFP
Plasmid#162446PurposeExpression in HEK293T cell and compete ligand singaling against full-length receptorsDepositorInsertC-X-C chemokine receptor type 4 (CXCR4 Human)
UseTagssfGFPExpressionMammalianMutationtruncation from aa52 to aa257PromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MAPKAPK3
Plasmid#23790DepositorInsertMAPKAPK3 (MAPKAPK3 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pOPINB-M1-di-Ub(G76V)-HOIL-1
Plasmid#229539PurposeConstitutively active M1-di-ubiquitin HOIL-1 fusion protein for expression in E. coliDepositorUseTags6xHis-3CExpressionBacterialMutationChanged Gly 76 to Val and changed Gly 76 to ValPromoterT7Available sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_CXCR4_WT
Plasmid#81957PurposeGateway Donor vector containing CXCR4, part of the Target Accelerator Plasmid Collection.DepositorInsertCXCR4 (CXCR4 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag ILKAP
Plasmid#20499DepositorInsertILKAP (ILKAP Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-hAP1 420-440
Plasmid#183643PurposeTo (ectopically) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; mammalian expressionDepositorInsertATP6AP1 (ATP6AP1 Human)
UseTagsmycExpressionMammalianMutationtruncated ATP6AP1 mutant; contains only the trans…PromoterCMVAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZAK
Plasmid#23709DepositorInsertZAK (MAP3K20 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hAP1-C-LAMP2 TM
Plasmid#183645PurposeLentiviral expression of a mutant of ATP6AP1 with its PEN2-interacting domain replaced with the transmembrane (TM) domain of LAMP2.DepositorUseLentiviralTagsHAExpressionMutationATP6AP1 transmembrane domain replaced by the tran…PromoterCMVAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL_THOC6:1-341
Plasmid#210927PurposeInsect expression of full length human THOC6. N-terminal 6X His tag with TEV cleavage.DepositorInsertTHOC6:M1-F341 (THOC6 Human)
UseTagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNLF1-C_PLCG1:1-1291
Plasmid#211089PurposeMammalian expression of full-length human PLCG1 isoform 2. C-terminal NanoLuc luciferase tag.DepositorInsertPLCG1:M1-L1291 (PLCG1 Human)
UseTagsNanoLuc luciferaseExpressionMammalianMutationisoform 2. I813T natural variant (VAR_011908)PromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
CCSB-Broad Human Kinase ORF Collection
Plasmid Kit#1000000014Purpose556 distinct human kinases and kinase-related protein ORFs in a pDONR-223 Entry Gateway vector. All clones are clonal isolates.DepositorAvailable sinceJuly 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
Target Accelerator Genetic Pathway Reference Set
Plasmid Kit#1000000105PurposeCollection of genes and mutations for reference pathway signatures and phenotypes to compare with those derived from cancer alleles; represent over 25 cellular signaling pathways and processesDepositorAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Myristoylated Kinase Library
Plasmid Kit#1000000012Purpose190 human kinases and kinase-related open reading frames (ORFs) cloned into a retroviral vector which adds a myristoylation sequence and flag-epitope tag to each ORF.DepositorAvailable sinceJuly 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
RAS Pathway 2.0 Clone Collection
Plasmid Kit#1000000070PurposeCollection contains 180 genes in the âRAS pathway version 2.0â, a crowdsourced pathway map of the RAS signaling pathway; For use with Gateway cloning or FNLCR Combinatorial Cloning PlatformDepositorAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Target Accelerator Lung Cancer Mutant Collection
Plasmid Kit#1000000104PurposePanel of lung adenocarcinoma alleles that have undergone basic, functional screening; full-length human clones (180 mutant and 45 corresponding wild-type) and 82 wild-type experssion controlsDepositorAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only