-
Plasmid#158491PurposeEntry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 without STOP codonDepositorInsertZAR1
UseGolden gate entry vectorTagsExpressionMutationno STOP codonPromoterAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA7
Plasmid#158485PurposeEntry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E with STOP codonDepositorInsertZAR1
UseGolden gate entry vectorTagsExpressionMutationL17E, STOP codonPromoterAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA15
Plasmid#158493PurposeEntry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E without STOP codonDepositorInsertZAR1
UseGolden gate entry vectorTagsExpressionMutationL17E, no STOP codonPromoterAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA6
Plasmid#158484PurposeEntry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V with STOP codonDepositorInsertZAR1
UseGolden gate entry vectorTagsExpressionMutationD481V, STOP codonPromoterAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA5
Plasmid#158483PurposeEntry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 with STOP codonDepositorInsertZAR1
UseGolden gate entry vectorTagsExpressionMutationSTOP codonPromoterAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA14
Plasmid#158492PurposeEntry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V without STOP codonDepositorInsertZAR1
UseGolden gate entry vectorTagsExpressionMutationD481V, no STOP codonPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV10.F6.loxP
Plasmid#63201PurposepCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV10.F3.loxP
Plasmid#63200PurposepCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAV10.K6.loxP
Plasmid#63203PurposepCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAV10.HO5.loxP
Plasmid#63206PurposepCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pLPE1
Plasmid#209981PurposeContains Level 0 Part: E. coli shuttle part (pMB1_ampR_B0017) for the construction of Level 1 plasmidsDepositorInsertE. coli shuttle part (pMB1_ampR_B0017)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPE2
Plasmid#209982PurposeContains Level 0 Part: E. coli shuttle part (ampR_B0017) for the construction of Level 1 plasmidsDepositorInsertE. coli shuttle part (ampR_B0017)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPE3
Plasmid#209985PurposeContains Level 0* Part: E. coli shuttle part (pMB1_cat) for the construction of Level 2 plasmidsDepositorInsertE. coli shuttle part (pMB1_cat)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPP5
Plasmid#209967PurposeContains Level 0 Part: inducible Promoter (PsppQ) with two regulatory genes sppK and sppR for the construction of Level 1 plasmidsDepositorInsertPromoter (P_sppQ)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPP6
Plasmid#209968PurposeContains Level 0 Part: Promoter Dropout (sfGFP) for the construction of Level 1 plasmids and subsequent promoter insertionDepositorInsertPromoter Dropout (sfGFP)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLP-mScarlet-I
Plasmid#209993PurposeContains Level 0 Part: Coding sequences (mScarlet-I) for the construction of Level 1 plasmidsDepositorInsertmScarlet-I
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPC53
Plasmid#209962PurposeContains Level 0 Part: 5' Connector (5C1CSN with double terminator Tcat+sai) for the construction of Level 1 plasmidsDepositorInsert5' Connector (5C1CSN_Tcat+sai)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGA-blue
Plasmid#196336PurposeEasy-MISE toolkit Level 0 destination plasmid for parts cloning with Esp3I-based Golden Gate Assembly; allowing fast postitive clones test with AmilCP chromoprotein-based blue/white screeningDepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assembly backboneTagsExpressionMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGGDestSC+ATG
Plasmid#49323PurposeDestination vector for Golden Gate assembly; Few restriction sites for subcloning.DepositorTypeEmpty backboneUseDestination vectorTagsExpressionMutationPromoterAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
p5E_GGWDest+
Plasmid#49319PurposeDestination vector for Golden Gate assembly; 5' entry vector for Gateway cloningDepositorTypeEmpty backboneUseDestination vectorTagsExpressionMutationPromoterAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only