We narrowed to 22,701 results for: Sele

1. pEBTet-SNAP-Cep97

   Plasmid

   #136800

   Purpose

   Mammalian expression of the centrosomal protein Cep97 N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-Cep97 (CEP97 Human, Synthetic)
   

   Use

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEBTetBl-CLIP-Cep128

   Plasmid

   #136872

   Purpose

   Mammalian expression of the centrosomal protein Cep128 N-terminally fused to CLIP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   CLIP-Cep128 (CEP128 Human, Synthetic)
   

   Use

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. GPR1-DuET

   Plasmid

   #213257

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPR1 (GPR1 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. plko-shmfn2-mfn2-Rescue

   Plasmid

   #160655

   Purpose

   "Rescue mfn2 expression by adding the guide-resistant-mutant mfn2 sequence"

   Depositor

   Qing Deng

   Article

   Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

   Insert

   mfn2 (MFN2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Agarabi CHO Heavy Chain in pJ201

   Plasmid

   #111567

   Purpose

   Cloning vector containing CDS for the modified human heavy chain of the model chimeric mouse-human IgG1 expressed by the Agarabi CHO cell line

   Depositor

   Cyrus Agarabi

   Article

   Velugula-Yellela et al Biotechnol Prog. 2018 Jan;34(1):262-270.

   Insert

   Human heavy chain
   

   Use

   Synthetic Biology; Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. HTR2C-DuET

   Plasmid

   #213323

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   HTR2C (HTR2C Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GPRC5D-DuET

   Plasmid

   #213298

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPRC5D (GPRC5D Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAC95-pmax-dCas9VP160-2A-neo

   Plasmid

   #48227

   Purpose

   dCas9VP160-2A-neo (neo/G418-selectable) on pmax expression vector. Note: This is being tested.

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-neo
   

   Use

   CRISPR

   Tags

   2A-neo, HA Tag, and VP160

   Expression

   Mammalian

   Mutation

   D10A;H840A (catalytically inactive)

   Promoter

   CAGGS

   Available since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GPR55-DuET

   Plasmid

   #213285

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPR55 (GPR55 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pIRES:mTREK-1(G137I)

    Plasmid

    #133274

    Purpose

    Mammalian expression vector. It will generate the mouse TREK-1 channel full lenght G137I mutant fused to a N-terminal HA tag

    Depositor

    Dan Minor

    Article

    Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

    Insert

    Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    G137I

    Promoter

    CMVd2

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HCTR1-DuET

    Plasmid

    #213310

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    HCTR1 (HCRTR1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HTR2A-DuET

    Plasmid

    #213321

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    HTR2A (HTR2A Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJM186A

    Plasmid

    #218127

    Purpose

    Hybrid circuit displaying hsCRBN bait and driving expression of gIII

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Inserts

    CRBN (CRBN Human)
    M13 pIII
    

    Use

    Tags

    434cI_RR69

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GPR171-DuET

    Plasmid

    #213269

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPR171 (GPR171 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HTR4-DuET

    Plasmid

    #213324

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    HTR4 (HTR4 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-hSyn-DIO-GRAB_UCN1.0

    Plasmid

    #208668

    Purpose

    Expresses the genetically-encoded fluorescent urocortin (UCN) sensor GRAB_UCN1.0 in neurons

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based urocortin (UCN) sensor GRAB_UCN1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDisplay-GRAB_NPYmut

    Plasmid

    #208678

    Purpose

    Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) control sensor GRAB_NPYmut in mammalian cells

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based neuropeptide Y (NPY) control sensor GRAB_NPYmut
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MCHR1-DuET

    Plasmid

    #213337

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    MCHR1 (MCHR1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMYs-MFN2-IRES-mitoRFP

    Plasmid

    #121997

    Purpose

    Expression of MFN2 and mitoRFP in mammalial cells

    Depositor

    Qing Deng

    Article

    Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

    Insert

    MFN2-IRES-mitoRFP (MFN2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    silent Q386Q Q387Q V388V Y389Y C390C E391E E392E

    Promoter

    Available since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEBTet-SNAP-Cep78

    Plasmid

    #136827

    Purpose

    Mammalian expression of the centrosomal protein Cep78 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Cep78 (CEP78 Human, Synthetic)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge