We narrowed to 4,154 results for: pCMV

1. pMR-A-3-Sasg

   Plasmid

   #214349

   Purpose

   Expresses T7-RNAP, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   T7-RNAP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Galt1-HT7-mGold

   Plasmid

   #230027

   Purpose

   Expressing fluorescent HaloTag7 fusion protein in mammalian cells with localization in the Golgi apparatus.

   Depositor

   Pablo Rivera-Fuentes

   Insert

   Galt1-HaloTag7-mGold (B4GALT1 Human, Synthetic)
   

   Use

   Tags

   HaloTag,mGold

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKJ458

   Plasmid

   #228709

   Purpose

   Mammalian expression of Antibody C143 HC R39K (heavy chain); to be used with pKJ460 (Plasmid 228710) to make the antibody

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Jiang et al Science 0, eadr6006

   Insert

   C143 HC R39K
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKJ460

   Plasmid

   #228710

   Purpose

   Mammalian expression of Antibody C143 LC N28R (light chain); to be used with pKJ458 (Plasmid 228709) to make the antibody

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Jiang et al Science 0, eadr6006

   Insert

   C143 LC N28R
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKJ423

   Plasmid

   #228708

   Purpose

   Mammalian expression of Anti-CD71 LC (light chain); to be used with pKJ455 (Plasmid 228707) to make the antibody

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Jiang et al Science 0, eadr6006

   Insert

   Anti-CD71 LC
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEJS2701-CMV-Nme2Smu-ABE8e-nt-E932R

   Plasmid

   #228722

   Purpose

   Expresses N-terminal ABE8e-Nme2Smu nCas9 (D16A) base editor with E932R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   N-terminal ABE8e with Nme2Smu nCas9 (D16A) E932R
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9, Smu PID, D16A E932R

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEJS2702-CMV-iNme2-ABE8e-nt

   Plasmid

   #228723

   Purpose

   Expresses N-terminal ABE8e-iNme2 nCas9 (D16A) base editor in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   N-terminal ABE8e with iNme2 nCas9 (D16A)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9 D16A D844G E868K K870R D873A D911G K929…

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEJS2703-CMV-iNme2Smu-ABE8e-nt

   Plasmid

   #228724

   Purpose

   Expresses N-terminal ABE8e-iNme2Smu nCas9 (D16A) base editor in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   N-terminal ABE8e with iNme2Smu nCas9 (D16A)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEJS2711-CMV-Nme2-ABE8e-i1-L10-D56R

   Plasmid

   #228732

   Purpose

   Expresses domain inlaid Nme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 and D56R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid Nme2 nCas9 (D16A)-i1-L10-D56R
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9 D16A D56R

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEJS2710-CMV-Nme2-ABE8e-i1-L10-E932R

    Plasmid

    #228731

    Purpose

    Expresses domain inlaid Nme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Domain inlaid Nme2 nCas9 (D16A)-i1-L10-E932R
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9 D16A E932R

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TL1 DdCBE A1 (DddA6-C)

    Plasmid

    #221992

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-T-TL1 TALE A1 repeats-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    DddA: T1413I

    Promoter

    CMV

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. TL1 DdCBE T2 (DddA6-N)

    Plasmid

    #221993

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-T-TL1 TALE T2 repeats-TALE CTD-DddA6 1397N-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    DddA: Q1310R, S1330I, T1380I

    Promoter

    CMV

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TL1 alphaDdCBE T2 (DddA6-N)

    Plasmid

    #221995

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE T2 repeats-TALE CTD-DddA6 1397N-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    TALE: Q231R, W232G, S233A | DddA: Q1310R, S1330I,…

    Promoter

    CMV

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ATP6 alphaDdCBE G2

    Plasmid

    #221959

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE G2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    TALE: Q231R, W232G, S233A, See depositor comments

    Promoter

    CMV

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TDP-43 mCerulean

    Plasmid

    #216227

    Purpose

    TDP-43 with a C-terminal mCerulean tag for expression in mammalian cells.

    Depositor

    Jennifer Lee

    Article

    Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. RGG-BcLOV-mCh-SOS

    Plasmid

    #208278

    Purpose

    Expresses RGG-BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.

    Depositor

    Lukasz Bugaj

    Article

    Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

    Insert

    RGG-BcLOV-mCh-SOS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    pCMV

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. RGG-BcLOV-mCh-EGFR

    Plasmid

    #208280

    Purpose

    Expresses RGG-BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.

    Depositor

    Lukasz Bugaj

    Article

    Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

    Insert

    RGG-BcLOV-mCh-EGFR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    H225N in mCherry- please see depositor comment

    Promoter

    pCMV

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Fus-BcLOV-mCh-EGFR

    Plasmid

    #208281

    Purpose

    Expresses Fus-BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.

    Depositor

    Lukasz Bugaj

    Article

    Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

    Insert

    Fus-BcLOV-mCh-EGFR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    pCMV

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Talin-T58-Cterminal-mEmerald

    Plasmid

    #202353

    Purpose

    Talin(DeltaR4-10)-mEmerald

    Depositor

    Pakorn Kanchanawong

    Article

    Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

    Insert

    Talin(DeltaR4-10)-mEmerald
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Talin-T36-Cterminal-mEmerald

    Plasmid

    #202357

    Purpose

    Talin(DeltaR2-12)-mEmerald

    Depositor

    Pakorn Kanchanawong

    Article

    Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

    Insert

    Talin(DeltaR2-12)-mEmerald
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge